Stimulation of Glycogenolysis in Isolated Hepatocytes by Adenosine and One of Its Analogues is Inhibited by Caffeine

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Nov 1

PMID 3426562

Citations 7

Authors

J C Stanley

J Markovic

A M Gutknecht

F J Lozeman

Affiliations

Soon will be listed here.

Abstract

The adenosine analogues 5'-(N-ethyl)carboxamidoadenosine (NECA) and N6-(phenylisopropyl)adenosine (PIA) activate glycogen phosphorylase 5-fold and 4.2-fold respectively in rat hepatocytes incubated in the absence of endogenous adenosine. Half-maximally effective concentrations are 0.5 microM for NECA and 20 microM for PIA, demonstrating the presence of A2-adenosine receptors. Exogenous adenosine activates phosphorylase 4.6-fold, but high rates of adenosine disappearance from the medium render estimates of its half-maximally effective concentration unreliable. These effects of NECA and adenosine are inhibited by 0.1 mM-caffeine. Activation of phosphorylase by a physiological concentration of adenosine (3.3 microM) was 50% inhibited by a physiological concentration of caffeine (35 microM).

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References

Bellet S, KERSHBAUM A, Finck E . Response of free fatty acids to coffee and caffeine. Metabolism. 1968; 17(8):702-7. DOI: 10.1016/0026-0495(68)90054-1. View

Butcher R, SUTHERLAND E . Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine. J Biol Chem. 1962; 237:1244-50. View

GILBOE D, Larson K, Nuttall F . Radioactive method for the assay of glycogen phosphorylases. Anal Biochem. 1972; 47(1):20-7. DOI: 10.1016/0003-2697(72)90274-6. View

Schwabe U, Ebert R, Erbler H . Adenosine release from fat cells: effect on cyclic AMP levels and hormone actions. Adv Cyclic Nucleotide Res. 1975; 5:569-84. View

Lund P, Cornell N, KREBS H . Effect of adenosine on the adenine nucleotide content and metabolism of hepatocytes. Biochem J. 1975; 152(3):593-9. PMC: 1172513. DOI: 10.1042/bj1520593. View

Lederer B, Stalmans W . Human liver glycogen phosphorylase. Kinetic properties and assay in biopsy specimens. Biochem J. 1976; 159(3):689-95. PMC: 1164170. DOI: 10.1042/bj1590689. View

van de Werve G, Hue L, HERS H . Hormonal and ionic control of the glycogenolytic cascade in rat liver. Biochem J. 1977; 162(1):135-42. PMC: 1164576. DOI: 10.1042/bj1620135. View

Wurzner H, Lindstrom E, Vuataz L . A 2-year feeding study of instant coffees in rats. I. Body weight, food consumption, haematological parameters and plasms chemistry. Food Cosmet Toxicol. 1977; 15(1):7-16. DOI: 10.1016/s0015-6264(77)80256-3. View

Kasvinsky P, MADSEN N, Sygusch J, Fletterick R . The regulation of glycogen phosphorylase alpha by nucleotide derivatives. Kinetic and x-ray crystallographic studies. J Biol Chem. 1978; 253(9):3343-51. View

10.

Acheson K, Zahorska-Markiewicz B, Pittet P, Anantharaman K, Jequier E . Caffeine and coffee: their influence on metabolic rate and substrate utilization in normal weight and obese individuals. Am J Clin Nutr. 1980; 33(5):989-97. DOI: 10.1093/ajcn/33.5.989. View

11.

Cornell N . Rapid fractionation of cell suspensions with the use of brominated hydrocarbons. Anal Biochem. 1980; 102(2):326-31. DOI: 10.1016/0003-2697(80)90162-1. View

12.

BERNE R, Belloni L, Rubio R . Adenosine associated with dispersed hepatocytes. Basic Res Cardiol. 1981; 76(4):377-9. DOI: 10.1007/BF01908326. View

13.

Kasvinsky P, Fletterick R, MADSEN N . Regulation of the dephosphorylation of glycogen phosphorylase a and synthase b by glucose and caffeine in isolated hepatocytes. Can J Biochem. 1981; 59(6):387-95. DOI: 10.1139/o81-054. View

14.

von Borstel R, Wurtman R, Conlay L . Chronic caffeine consumption potentiates the hypotensive action of circulating adenosine. Life Sci. 1983; 32(10):1151-8. DOI: 10.1016/0024-3205(83)90121-2. View

15.

Bartrons R, Van Schaftingen E, HERS H . The ability of adenosine to decrease the concentration of fructose 2,6-bisphosphate in isolated hepatocytes. A cyclic AMP-mediated effect. Biochem J. 1984; 218(1):157-63. PMC: 1153319. DOI: 10.1042/bj2180157. View

16.

Leach R, Allan E, Titheradge M . The stimulation of glycogenolysis in isolated hepatocytes by opioid peptides. Biochem J. 1985; 227(1):191-7. PMC: 1144825. DOI: 10.1042/bj2270191. View

17.

Daly J . Adenosine receptors. Adv Cyclic Nucleotide Protein Phosphorylation Res. 1985; 19:29-46. View

18.

Keppens S, DE WULF H . P2-purinergic control of liver glycogenolysis. Biochem J. 1985; 231(3):797-99. PMC: 1152822. DOI: 10.1042/bj2310797. View

19.

Hoffer L, Lowenstein J . Effects of adenosine and adenosine analogues on glycogen metabolism in isolated rat hepatocytes. Biochem Pharmacol. 1986; 35(24):4529-36. DOI: 10.1016/0006-2952(86)90775-6. View

20.

Buxton D, Robertson S, Olson M . Stimulation of glycogenolysis by adenine nucleotides in the perfused rat liver. Biochem J. 1986; 237(3):773-80. PMC: 1147056. DOI: 10.1042/bj2370773. View