Fluorescent Nanodiamond - Hyaluronate Conjugates for Target-specific Molecular Imaging

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2021 Jul 15

PMID 34262698

Citations 2

Authors

Hye Hyeon Han

Homan Kang

Seong-Jong Kim

Rahul Pal

Anand T N Kumar

Hak Soo Choi

Sei Kwang Hahn

Affiliations

Soon will be listed here.

Abstract

Despite wide investigation on molecular imaging contrast agents, there are still strong unmet medical needs to enhance their signal-to background ratio, brightness, photostability, and biocompatibility with multimodal imaging capability. Here, we assessed the feasibility of fluorescent nanodiamonds (FNDs) as carbon based photostable and biocompatible materials for molecular imaging applications. Because FNDs have negatively charged nitrogen vacancy (NV) centers, they can emit bright red light. FNDs were conjugated to hyaluronate (HA) for target-specific molecular imaging. HA is a biocompatible, biodegradable, and linear polysaccharide with abundant HA receptors in the liver, enabling liver targeted molecular imaging. cell viability tests revealed the biocompatibility of HA-FND conjugates and the competitive cellular uptake test confirmed their target-specific intracellular delivery to HepG2 cells with HA receptors. In addition, fluorescence lifetime (FLT) assessment revealed the imaging capability of FNDs and HA-FND conjugates. After that, we could confirm the statistically significant liver-targeted delivery of HA-FND conjugates by imaging system (IVIS) analysis and biodistribution tests in various organs. The renal clearance test and histological analysis corroborated the biocompatibility and safety of HA-FND conjugates. All these results demonstrated the feasibility of HA-FND conjugates for further molecular imaging applications.

Citing Articles

Production, surface modification, physicochemical properties, biocompatibility, and bioimaging applications of nanodiamonds.

Tegafaw T, Liu S, Ahmad M, Saidi A, Zhao D, Liu Y RSC Adv. 2023; 13(46):32381-32397.

PMID: 37928839 PMC: 10623544. DOI: 10.1039/d3ra06837d.

Comparison of fluorescence lifetime and multispectral imaging for quantitative multiplexing in biological tissue.

Pal R, Kumar A Biomed Opt Express. 2022; 13(7):3854-3868.

PMID: 35991924 PMC: 9352286. DOI: 10.1364/BOE.459935.

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