Three-dimensional Human Placenta-like Bud Synthesized from Induced Pluripotent Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2021 Jul 9

PMID 34239021

Citations 3

Authors

Mai Sato

Asako Inohaya

Eriko Yasuda

Haruta Mogami

Yoshitsugu Chigusa

Kaoru Kawasaki

Yosuke Kawamura

Yusuke Ueda

Hiroshi Takai

Masaki Mandai

Eiji Kondoh

Affiliations

Soon will be listed here.

Abstract

Placental dysfunction is related to the pathogenesis of preeclampsia and fetal growth restriction, but there is no effective treatment for it. Recently, various functional three-dimensional organs have been generated from human induced-pluripotent cells (iPSCs), and the transplantation of these iPSCs-derived organs has alleviated liver failure or diabetes mellitus in mouse models. Here we successfully generated a three-dimensional placental organ bud from human iPSCs. The iPSCs differentiated into various lineages of trophoblasts such as cytotrophoblast-like, syncytiotrophoblast-like, and extravillous trophoblast-like cells, forming organized layers in the bud. Placental buds were transplanted to the murine uterus, where 22% of the buds were successfully engrafted. These iPSC-derived placental organ buds could serve as a new model for the study of placental function and pathology.

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