Biofabrication of Gold Nanoparticles Using Extract and Its Antiquorum Sensing and Antibiofilm Activity Against Bacterial Pathogens

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2021 Jul 8

PMID 34235339

Citations 15

Authors

Faizan Abul Qais

Iqbal Ahmad

Mohammad Altaf

Saad H Alotaibi

Affiliations

Soon will be listed here.

Abstract

The intensive use of antimicrobial agents has led to the emergence of multidrug resistance (MDR) among microbial pathogens. Such microbial (MDR) infections become more problematic in chronic diseases in which the efficacy of chemotherapeutic agents is highly reduced. To combat the problem of drug resistance, inhibition of bacterial quorum sensing (QS) and biofilms are considered as promising strategies in the development of anti-infective agents. In this study, gold nanoparticles (AuNPs-CA) were biofabricated using aqueous extract and characterized. The AuNPs-CA were tested against the QS-controlled virulence factors and biofilms of PAO1 and MTCC 97. AuNPs-CA were found to be crystalline in nature with average particle size 19.97 nm. QS-mediated virulent traits of PAO1 such as pyocyanin, pyoverdin, exoprotease activity, elastase activity, rhamnolipids production, and swimming motility were reduced by 91.94, 72.16, 81.82, 65.72, 46.66, and 46.09%, respectively. Similarly, dose-dependent inhibition of virulence factors of MTCC 97 was recorded by the treatment of AuNPs-CA. The biofilm development and exopolysaccharide (EPS) production also decreased significantly. Microscopic analysis revealed that the adherence and colonization of the bacteria on solid support were reduced to a remarkable extent. The findings indicate the possibility of application of green synthesized gold nanoparticles in the management of bacterial infection after careful in vivo investigation.

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References

Shakoor S, Platts-Mills J, Hasan R . Antibiotic-Resistant Enteric Infections. Infect Dis Clin North Am. 2019; 33(4):1105-1123. DOI: 10.1016/j.idc.2019.05.007. View

Kessler E, Israel M, Landshman N, Chechick A, Blumberg S . In vitro inhibition of Pseudomonas aeruginosa elastase by metal-chelating peptide derivatives. Infect Immun. 1982; 38(2):716-23. PMC: 347797. DOI: 10.1128/iai.38.2.716-723.1982. View

Fothergill J, Panagea S, A Hart C, Walshaw M, Pitt T, Winstanley C . Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain. BMC Microbiol. 2007; 7:45. PMC: 1890549. DOI: 10.1186/1471-2180-7-45. View

Bhardwaj V, Kaushik A . Biomedical Applications of Nanotechnology and Nanomaterials. Micromachines (Basel). 2018; 8(10). PMC: 6190473. DOI: 10.3390/mi8100298. View

Essar D, Eberly L, Hadero A, Crawford I . Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol. 1990; 172(2):884-900. PMC: 208517. DOI: 10.1128/jb.172.2.884-900.1990. View

Rutherford S, Bassler B . Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med. 2012; 2(11). PMC: 3543102. DOI: 10.1101/cshperspect.a012427. View

Courtney H, Ofek I, Penfound T, Nizet V, Pence M, Kreikemeyer B . Relationship between expression of the family of M proteins and lipoteichoic acid to hydrophobicity and biofilm formation in Streptococcus pyogenes. PLoS One. 2009; 4(1):e4166. PMC: 2613554. DOI: 10.1371/journal.pone.0004166. View

Lopez D, Vlamakis H, Kolter R . Biofilms. Cold Spring Harb Perspect Biol. 2010; 2(7):a000398. PMC: 2890205. DOI: 10.1101/cshperspect.a000398. View

Rau J, De Santis R, Ciofani G . Exploring challenges ahead of nanotechnology for biomedicine. Bioact Mater. 2018; 2(3):119-120. PMC: 5935177. DOI: 10.1016/j.bioactmat.2017.09.004. View

10.

OToole G, Kolter R . Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol. 1998; 30(2):295-304. DOI: 10.1046/j.1365-2958.1998.01062.x. View

11.

Samanta S, Singh B, Adholeya A . Intracellular Synthesis of Gold Nanoparticles Using an Ectomycorrhizal Strain EM-1083 of and Its Nanoanti-quorum Sensing Potential Against . Indian J Microbiol. 2017; 57(4):448-460. PMC: 5671422. DOI: 10.1007/s12088-017-0662-4. View

12.

Goluszko P, Nowicki B, Goluszko E, Nowicki S, Kaul A, Pham T . Association of colony variation in Serratia marcescens with the differential expression of protease and type 1 fimbriae. FEMS Microbiol Lett. 1995; 133(1-2):41-5. DOI: 10.1111/j.1574-6968.1995.tb07858.x. View

13.

Das T, Kutty S, Tavallaie R, Ibugo A, Panchompoo J, Sehar S . Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation. Sci Rep. 2015; 5:8398. PMC: 4323658. DOI: 10.1038/srep08398. View

14.

Kalia V . Quorum sensing inhibitors: an overview. Biotechnol Adv. 2012; 31(2):224-45. DOI: 10.1016/j.biotechadv.2012.10.004. View

15.

Percival S, Hill K, Williams D, Hooper S, Thomas D, Costerton J . A review of the scientific evidence for biofilms in wounds. Wound Repair Regen. 2012; 20(5):647-57. DOI: 10.1111/j.1524-475X.2012.00836.x. View

16.

Slater H, Crow M, Everson L, Salmond G . Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways. Mol Microbiol. 2003; 47(2):303-20. DOI: 10.1046/j.1365-2958.2003.03295.x. View

17.

Shkodenko L, Kassirov I, Koshel E . Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations. Microorganisms. 2020; 8(10). PMC: 7601517. DOI: 10.3390/microorganisms8101545. View

18.

Saeki E, Kobayashi R, Nakazato G . Quorum sensing system: Target to control the spread of bacterial infections. Microb Pathog. 2020; 142:104068. DOI: 10.1016/j.micpath.2020.104068. View

19.

Rajkumari J, Busi S, Vasu A, Reddy P . Facile green synthesis of baicalein fabricated gold nanoparticles and their antibiofilm activity against Pseudomonas aeruginosa PAO1. Microb Pathog. 2017; 107:261-269. DOI: 10.1016/j.micpath.2017.03.044. View

20.

Cox C, Adams P . Siderophore activity of pyoverdin for Pseudomonas aeruginosa. Infect Immun. 1985; 48(1):130-8. PMC: 261925. DOI: 10.1128/iai.48.1.130-138.1985. View