» Articles » PMID: 34235203

Use of Field Based Loop Mediated Isothermal Amplification (LAMP) Technology for a Prevalence Survey and Proof of Freedom Survey for African Swine Fever in Timor-Leste in 2019

Overview
Journal Front Vet Sci
Date 2021 Jul 8
PMID 34235203
Citations 4
Authors
Affiliations
Soon will be listed here.
Abstract

African Swine Fever (ASF) has been spreading in numerous southeast Asian countries since a major incursion in mainland China in 2018. Timor-Leste confirmed an outbreak of ASF in September 2019 which resulted in high mortalities in affected pigs. Pigs in Timor-Leste are the second most common type of livestock kept by villagers and represent a traditionally important source of income and prestige for householders. In order to understand the extent of ASF infected villages in Timor-Leste a prevalence survey was designed and conducted in November-December 2019. Timor-Leste has limited laboratory facilities and access to qPCR diagnostic tests. Therefore, a loop mediated isothermal amplification (LAMP) assay was used to detect ASF positive blood samples collected during the prevalence survey. The LAMP assay was proven to be a robust, highly specific and sensitive laboratory test for ASF suitable for use in the field and where there are limited laboratory facilities. The results of the prevalence survey allowed the extent of the ASF incursion to be delineated and the introduction of a disease response strategy to limit the spread of ASF and assist in the recovery of the pig population in Timor-Leste.

Citing Articles

Transboundary viral diseases of pigs, poultry and ruminants in Southeast Asia: a systematic review.

Mai T, Nguyen T, Dang-Xuan S, Nguyen-Viet H, Unger F, Lee H Vet Q. 2024; 44(1):13-25.

PMID: 39210836 PMC: 11370669. DOI: 10.1080/01652176.2024.2397796.


Vaccines for African swine fever: an update.

Zhang H, Zhao S, Zhang H, Qin Z, Shan H, Cai X Front Microbiol. 2023; 14:1139494.

PMID: 37180260 PMC: 10173882. DOI: 10.3389/fmicb.2023.1139494.


Community engagement strengthens pig disease knowledge and passive surveillance in Timor-Leste.

Millar J, Morais O, Da Silva H, Hick P, Foster A, Jong J Front Vet Sci. 2023; 9:1024094.

PMID: 36713866 PMC: 9878314. DOI: 10.3389/fvets.2022.1024094.


Rapid and sensitive detection of pathogenic in black spotted frog by RPA-LFD and fluorescent probe-based RPA.

Qiao M, Zhang L, Chang J, Li H, Li J, Wang W Fish Shellfish Immunol Rep. 2022; 3:100059.

PMID: 36419595 PMC: 9680066. DOI: 10.1016/j.fsirep.2022.100059.

References
1.
Sawford K, Karmo A, Conceicao F, Geong M, Tenaya I, Hartawan D . An investigation of classical swine fever virus seroprevalence and risk factors in pigs in Timor-Leste. Prev Vet Med. 2015; 122(1-2):99-106. DOI: 10.1016/j.prevetmed.2015.09.012. View

2.
Zhou X, Li N, Luo Y, Liu Y, Miao F, Chen T . Emergence of African Swine Fever in China, 2018. Transbound Emerg Dis. 2018; 65(6):1482-1484. DOI: 10.1111/tbed.12989. View

3.
James H, Ebert K, McGonigle R, Reid S, Boonham N, Tomlinson J . Detection of African swine fever virus by loop-mediated isothermal amplification. J Virol Methods. 2009; 164(1-2):68-74. DOI: 10.1016/j.jviromet.2009.11.034. View

4.
Mazur-Panasiuk N, Zmudzki J, Wozniakowski G . African Swine Fever Virus - Persistence in Different Environmental Conditions and the Possibility of its Indirect Transmission. J Vet Res. 2019; 63(3):303-310. PMC: 6749736. DOI: 10.2478/jvetres-2019-0058. View

5.
Barnes T, Morais O, Cargill C, Parke C, Urlings A . First steps in managing the challenge of African Swine Fever in Timor-Leste. One Health. 2020; 10:100151. PMC: 7582221. DOI: 10.1016/j.onehlt.2020.100151. View