Acceleration of Age-induced Proteolysis in the Guinea Pig Lens Nucleus by in Vivo Exposure to Hyperbaric Oxygen: A Mass Spectrometry Analysis

Overview

Journal Exp Eye Res

Specialty Ophthalmology

Date 2021 Jul 7

PMID 34233175

Citations 3

Authors

Frank J Giblin

David M G Anderson

Jun Han

Kristie L Rose

Zhen Wang

Kevin L Schey

Affiliations

Soon will be listed here.

Abstract

Hyperbaric oxygen (HBO) treatment of animals or ocular lenses in culture recapitulates many molecular changes observed in human age-related nuclear cataract. The guinea pig HBO model has been one of the best examples of such treatment leading to dose-dependent development of lens nuclear opacities. In this study, complimentary mass spectrometry methods were employed to examine protein truncation after HBO treatment of aged guinea pigs. Quantitative liquid chromatography-mass spectrometry (LC-MS) analysis of the membrane fraction of guinea pig lenses showed statistically significant increases in aquaporin-0 (AQP0) C-terminal truncation, consistent with previous reports of accelerated loss of membrane and cytoskeletal proteins. In addition, imaging mass spectrometry (IMS) analysis spatially mapped the acceleration of age-related αA-crystallin truncation in the lens nucleus. The truncation sites in αA-crystallin closely match those observed in human lenses with age. Taken together, our results suggest that HBO accelerates the normal lens aging process and leads to nuclear cataract.

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