The Effects of Exercise Training Versus Intensive Insulin Treatment on Skeletal Muscle Fibre Content in Type 1 Diabetes Mellitus Rodents

Overview

Journal Lipids Health Dis

Publisher Biomed Central

Specialties Biochemistry
Endocrinology

Date 2021 Jul 7

PMID 34229671

Citations 3

Authors

David P McBey

Michelle Dotzert

C W J Melling

Affiliations

Soon will be listed here.

Abstract

Background: Intensive-insulin treatment (IIT) strategy for patients with type 1 diabetes mellitus (T1DM) has been associated with sedentary behaviour and the development of insulin resistance. Exercising patients with T1DM often utilize a conventional insulin treatment (CIT) strategy leading to increased insulin sensitivity through improved intramyocellular lipid (IMCL) content. It is unclear how these exercise-related metabolic adaptations in response to exercise training relate to individual fibre-type transitions, and whether these alterations are evident between different insulin strategies (CIT vs. IIT).

Purpose: This study examined glycogen and fat content in skeletal muscle fibres of diabetic rats following exercise-training.

Methods: Male Sprague-Dawley rats were divided into four groups: Control-Sedentary, CIT- and IIT-treated diabetic sedentary, and CIT-exercised trained (aerobic/resistance; DARE). After 12 weeks, muscle-fibre lipids and glycogen were compared through immunohistochemical analysis.

Results: The primary findings were that both IIT and DARE led to significant increases in type I fibres when compared to CIT, while DARE led to significantly increased lipid content in type I fibres compared to IIT.

Conclusions: These findings indicate that alterations in lipid content with insulin treatment and DARE are primarily evident in type I fibres, suggesting that muscle lipotoxicity in type 1 diabetes is muscle fibre-type dependant.

Citing Articles

Mechanisms of insulin resistance in type 1 diabetes mellitus: A case of glucolipotoxicity in skeletal muscle.

Sammut M, Dotzert M, Melling C J Cell Physiol. 2024; 239(12):e31419.

PMID: 39192756 PMC: 11649966. DOI: 10.1002/jcp.31419.

The Effects of Resistance Exercise Training on Skeletal Muscle Metabolism and Insulin Resistance Development in Female Rodents with Type 1 Diabetes.

Sammut M, McBey D, Sayal A, Melling C J Diabetes Res. 2024; 2024:5549762.

PMID: 38435452 PMC: 10904684. DOI: 10.1155/2024/5549762.

A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes.

Mohsen R, Hassan R Saudi Dent J. 2023; 35(6):668-677.

PMID: 37817792 PMC: 10562111. DOI: 10.1016/j.sdentj.2023.05.020.

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