Development of a Novel Cell Surface Attachment System to Display Multi-Protein Complex Using the Cohesin-Dockerin Binding Pair

Overview

Journal J Microbiol Biotechnol

Specialties Biotechnology
Microbiology

Date 2021 Jul 6

PMID 34226404

Authors

Hyeok-Jin Ko

Heesang Song

In-Geol Choi

Affiliations

Soon will be listed here.

Abstract

Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display. While overcoming these drawbacks, we ultimately extended the applicability of the autodisplay platform to the display of a protein complex. We designed and constructed a cell surface attachment (CSA) system that uses a noncovalent protein-protein interaction. We employed the high-affinity interaction mediated by an orthogonal cohesin-dockerin (Coh-Doc) pair from to build the CSA system. Then, we validated the orthogonal Coh-Doc binding by attaching a monomeric red fluorescent protein to the cell surface. In addition, we evaluated the functional anchoring of proteins fused with the Doc module to the autodisplayed Coh module on the surface of . The designed CSA system was applied to create a functional attachment of dimeric α-neoagarobiose hydrolase to the surface of cells.

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