Prevalence and Characterization of MRSA in Bovine Bulk Tank Milk in Great Britain, 2017-18

Overview

Journal JAC Antimicrob Resist

Publisher Oxford University Press

Specialty Infectious Diseases

Date 2021 Jul 5

PMID 34223074

Citations 6

Authors

Cheng Cui

Xiaoliang Ba

Mark A Holmes

Affiliations

Soon will be listed here.

Abstract

Objectives: To evaluate the current prevalence status of MRSA among dairy farms in England and Wales 5 years after a previous survey conducted in 2011-12.

Methods: A convenience sample of 697 dairy farms in England and Wales was used for the study, conducted in 2017-18, testing bulk tank milk samples for the presence of MRSA using high salt broth enrichment and chromogenic MRSA agar selection. All putative MRSA isolates were screened by PCR for the presence of and genes and subjected to antimicrobial susceptibility testing using both the disc diffusion method and VITEK 2. MRSA isolates were also sequenced for genomic characterization.

Results: MRSA were detected on 4 out of 697 dairy farms in England and Wales (prevalence 0.57%, 95% CI 0.16%-1.46%). Three of the isolates were ST425 and one was ST4652 (in the CC130 lineage). Two MRSA were also isolated: one ST5 and one ST398.

Conclusions: These results indicate that there has been a substantial reduction in the prevalence of MRSA in England and Wales with a 72% reduction (2.15% to 0.57%) compared with a previous study. While the levels of MRSA remain very low the continued presence of ST398, a livestock-associated MRSA, suggests that this lineage is established in the UK.

Citing Articles

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Kinship analysis of gene of methicillin-resistant isolated from milk and risk factors from the farmers in Blitar, Indonesia.

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Kos A, Papic B, Golob M, Avbersek J, Kusar D, Ledina T Antibiotics (Basel). 2023; 12(10).

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Molecular Surveillance of MRSA in Raw Milk Provides Insight into MRSA Cross Species Evolution.

Zhang Z, Wang J, Wang H, Zhang L, Shang W, Li Z Microbiol Spectr. 2023; 11(4):e0031123.

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EFSA J. 2023; 21(3):e07867.

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