1,25-Dihydroxyvitamin D3 Transcriptionally Regulates Tumour Necrosis Factor MRNA During HL-60 Cell Differentiation

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1988 Jan 1

PMID 3422222

Citations 4

Authors

M Steffen

Y Cayre

K R Manogue

M A Moore

Affiliations

Soon will be listed here.

Abstract

We investigated TNF-alpha gene expression in HL-60 cells under the influence of 1,25(OH)2D3. Our results show that TNF-alpha gene expression is transcriptionally activated by 1,25(OH)2D3. Despite the increase of TNF-alpha mRNA, very little TNF protein is found in either supernatants or cell lysates of HL-60 cells cultured in the presence of the vitamin. Therefore 1,25(OH)2D3 is regulating the transcription of the TNF-alpha gene in HL-60 cells without affecting translation. Our data suggest that an additional signal is necessary under physiological conditions to control post-transcriptionally TNF-alpha protein synthesis and secretion.

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