Thermal Treatment Improves a Process of Crude Glycerol Valorization for the Production of a Heterologous Enzyme by

Overview

Journal Biotechnol Rep (Amst)

Specialty Biotechnology

Date 2021 Jul 5

PMID 34221911

Citations 6

Authors

Monika Kubiak

Wojciech Bialas

Ewelina Celinska

Affiliations

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Abstract

Valorization of crude glycerol requires a potent bifunctional biocatalyst, such as , capable of high-density growth on this substrate, and having high propensity for heterologous protein synthesis. Increasing evidence suggests that controlled administration of stress, thermal treatment, has a positive impact on bioprocess performance. In this study, we systematically adjusted thermal treatment conditions (20 to 42 °C) in order to maximize heterologous protein production by growing in crude glycerol-based medium. Our results showed nearly 30% enhancement in the enzyme production triggered by temporary exposure to decreased temperature. Here developed mathematical model indicated optimal treatment conditions (20 °C, 153') that were later applied to a process with biodiesel-derived glycerol and technical substrates. Techno-economic analysis of a pilot-scale-waste-free process was conducted. Quantitative description of the associated costs and economic gain due to exploitation of industrial substrates, as well as indication of current bottlenecks of the process, are also provided.

Citing Articles

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Transcription factors enhancing synthesis of recombinant proteins and resistance to stress in Yarrowia lipolytica.

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Hyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica-molecular background revealed by quantitative proteomics.

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