An Activity-based Fluorescent Sensor for the Detection of the Phenol Sulfotransferase SULT1A1 in Living Cells

Overview

Journal RSC Chem Biol

Publisher Royal Society of Chemistry

Specialty Biology

Date 2021 Jul 2

PMID 34212150

Citations 3

Authors

Regina A Baglia

Kira R Mills

Koushambi Mitra

Jasmine N Tutol

Darby Ball

Kierstin M Page

Jyothi Kallu

Sriharika Gottipolu

Sheena DArcy

Steven O Nielsen

Sheel C Dodani

Affiliations

Soon will be listed here.

Abstract

Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.

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