Effects of Dietary YFI-SC2 on the Growth Performance, Intestinal Morphology, Immune Parameters, Intestinal Microbiota, and Disease Resistance of Crayfish

Overview

Journal Animals (Basel)

Date 2021 Jul 2

PMID 34209070

Citations 7

Authors

Yan Xu

Yiqun Li

Mingyang Xue

Tao Yang

Xiaowen Luo

Yuding Fan

Yan Meng

Wenzhi Liu

Ge Lin

Bo Li

Lingbing Zeng

Yong Zhou

Affiliations

Soon will be listed here.

Abstract

The present study aimed to evaluate the effect of the dietary supplementation of YFI-SC2 on the growth performance, intestinal morphology, immune parameters, intestinal microbiota, and disease resistance of crayfish (). Crayfish were randomly assigned to six different boxes and two different groups in triplicate. The control group received a basal diet and the treatment group received a diet containing at 10 CFU/g. After feeding for 28 days, crayfish of the treatment group exhibited a significantly better weight gain ratio (WGR) and a specific growth rate (SGR) ( < 0.05) than crayfish of the control group. Compared to the treatment group, the control group intestines showed an oedema connective tissue layer and a weak muscle layer. For immune-related genes, Crustin2 expression was similar between the groups, whereas Lysozyme and prophenoloxidase from treatment group expression levels were upregulated significantly ( < 0.05) after 14 and 28 days of feeding. Prophenoloxidase showed the highest expression, with 10.5- and 8.2-fold higher expression than in the control group at 14 and 28 days, respectively. The intestinal microbiota community structure was markedly different between the two groups. After 14 and 28 days of feeding, the relative abundance of and increased, whereas and decreased in the treatment group compared with that of the control group. The challenge test showed that crayfish of the treatment group had a significantly enhanced resistance against ( < 0.05). Our results suggest that a -containing diet positively influenced the health status, immune parameters, intestinal microbiota composition, and disease resistance of crayfish.

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