A Comparative In Vitro Evaluation of the Anti-Inflammatory Effects of a Extract and Fucoxanthin

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2021 Jul 2

PMID 34207952

Citations 16

Authors

Elisabetta Bigagli

Mario DAmbrosio

Lorenzo Cinci

Alberto Niccolai

Natascia Biondi

Liliana Rodolfi

Luana Beatriz Dos Santos Nascimiento

Mario R Tredici

Cristina Luceri

Affiliations

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Abstract

In this study, we compared the effects of a   F&M-M36 methanolic extract with those of fucoxanthin (FX) at equivalent concentration, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The F&M-M36 methanolic extract contained 4.7 mg of FX and 6.22 mg of gallic acid equivalents of phenols per gram. HPLC analysis revealed the presence of simple phenolic acid derivatives. The F&M-M36 extract exhibited a potent and concentration-dependent inhibitory activity against COX-2 dependent PGE2 production compared to FX alone. Compared to LPS, F&M-M36 extract and FX reduced the expression of IL-6 and of Arg1 and enhanced that of IL-10 and of HO-1; F&M-M36 extract also significantly abated the expression of NLRP3, enhanced mir-223 expression and reduced that of mir-146b, compared to LPS ( < 0.05). These findings indicate that F&M-M36 methanolic extract has a peculiar anti-inflammatory activity against COX-2/PGE2 and NLRP3/mir-223 that might be attributable to the known anti-inflammatory effects of simple phenolic compounds found in the extract that may synergize with FX. Our data suggest that F&M-M36 may serve as a source of anti-inflammatory compounds to be further evaluated in in vivo models of inflammation.

Citing Articles

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References

Galvan-Pena S, ONeill L . Metabolic reprograming in macrophage polarization. Front Immunol. 2014; 5:420. PMC: 4151090. DOI: 10.3389/fimmu.2014.00420. View

Neudecker V, Haneklaus M, Jensen O, Khailova L, Masterson J, Tye H . Myeloid-derived miR-223 regulates intestinal inflammation via repression of the NLRP3 inflammasome. J Exp Med. 2017; 214(6):1737-1752. PMC: 5460990. DOI: 10.1084/jem.20160462. View

Luceri C, Bigagli E, Pitozzi V, Giovannelli L . A nutrigenomics approach for the study of anti-aging interventions: olive oil phenols and the modulation of gene and microRNA expression profiles in mouse brain. Eur J Nutr. 2015; 56(2):865-877. DOI: 10.1007/s00394-015-1134-4. View

Chen P, Li Y, Li L, Yu Q, Chao K, Zhou G . Circulating microRNA146b-5p is superior to C-reactive protein as a novel biomarker for monitoring inflammatory bowel disease. Aliment Pharmacol Ther. 2019; 49(6):733-743. DOI: 10.1111/apt.15159. View

Gallego R, Tardif C, Parreira C, Guerra T, Alves M, Ibanez E . Simultaneous extraction and purification of fucoxanthin from Tisochrysis lutea microalgae using compressed fluids. J Sep Sci. 2020; 43(9-10):1967-1977. DOI: 10.1002/jssc.202000021. View

Zhang N, Fu L, Bu Y, Yao Y, Wang Y . Downregulated expression of miR-223 promotes Toll-like receptor-activated inflammatory responses in macrophages by targeting RhoB. Mol Immunol. 2017; 91:42-48. DOI: 10.1016/j.molimm.2017.08.026. View

Bauernfeind F, Rieger A, Schildberg F, Knolle P, Schmid-Burgk J, Hornung V . NLRP3 inflammasome activity is negatively controlled by miR-223. J Immunol. 2012; 189(8):4175-81. DOI: 10.4049/jimmunol.1201516. View

Dunkhunthod B, Talabnin C, Murphy M, Thumanu K, Sittisart P, Hengpratom T . Intracellular ROS Scavenging and Anti-Inflammatory Activities of Kurz (L.) Extract in LPS plus IFN--Activated RAW264.7 Macrophages. Evid Based Complement Alternat Med. 2020; 2020:7436920. PMC: 7273479. DOI: 10.1155/2020/7436920. View

Yuan X, Berg N, Lee J, Le T, Neudecker V, Jing N . MicroRNA miR-223 as regulator of innate immunity. J Leukoc Biol. 2018; 104(3):515-524. PMC: 6638550. DOI: 10.1002/JLB.3MR0218-079R. View

10.

Batra S, Heier C, Diaz-Calderon L, Tully C, Fiorillo A, van den Anker J . Serum miRNAs Are Pharmacodynamic Biomarkers Associated With Therapeutic Response in Pediatric Inflammatory Bowel Disease. Inflamm Bowel Dis. 2020; 26(10):1597-1606. PMC: 7500519. DOI: 10.1093/ibd/izaa209. View

11.

Su J, Guo K, Huang M, Liu Y, Zhang J, Sun L . Fucoxanthin, a Marine Xanthophyll Isolated From ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis. Front Pharmacol. 2019; 10:906. PMC: 6722224. DOI: 10.3389/fphar.2019.00906. View

12.

Matos J, Cardoso C, Gomes A, Campos A, Fale P, Afonso C . Bioprospection of Isochrysis galbana and its potential as a nutraceutical. Food Funct. 2019; 10(11):7333-7342. DOI: 10.1039/c9fo01364d. View

13.

Bigagli E, Luceri C, Scartabelli T, Dolara P, Casamenti F, Pellegrini-Giampietro D . Long-term Neuroglial Cocultures as a Brain Aging Model: Hallmarks of Senescence, MicroRNA Expression Profiles, and Comparison With In Vivo Models. J Gerontol A Biol Sci Med Sci. 2015; 71(1):50-60. DOI: 10.1093/gerona/glu231. View

14.

Tan C, Hou Y . First evidence for the anti-inflammatory activity of fucoxanthin in high-fat-diet-induced obesity in mice and the antioxidant functions in PC12 cells. Inflammation. 2013; 37(2):443-50. DOI: 10.1007/s10753-013-9757-1. View

15.

Bigagli E, Cinci L, DAmbrosio M, Luceri C . Pharmacological activities of an eye drop containing Matricaria chamomilla and Euphrasia officinalis extracts in UVB-induced oxidative stress and inflammation of human corneal cells. J Photochem Photobiol B. 2017; 173:618-625. DOI: 10.1016/j.jphotobiol.2017.06.031. View

16.

Hwang P, Phan N, Lu W, Ngoc Hieu B, Lin Y . Low-molecular-weight fucoidan and high-stability fucoxanthin from brown seaweed exert prebiotics and anti-inflammatory activities in Caco-2 cells. Food Nutr Res. 2016; 60:32033. PMC: 4973444. DOI: 10.3402/fnr.v60.32033. View

17.

Kim M, Kang H, Li Y, Park Y, Lee J . Fucoxanthin inhibits lipopolysaccharide-induced inflammation and oxidative stress by activating nuclear factor E2-related factor 2 via the phosphatidylinositol 3-kinase/AKT pathway in macrophages. Eur J Nutr. 2021; 60(6):3315-3324. DOI: 10.1007/s00394-021-02509-z. View

18.

Castagnini C, Luceri C, Toti S, Bigagli E, Caderni G, Femia A . Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols. Br J Nutr. 2009; 102(11):1620-8. DOI: 10.1017/S0007114509990936. View

19.

Montana G, Lampiasi N . Substance P Induces HO-1 Expression in RAW 264.7 Cells Promoting Switch towards M2-Like Macrophages. PLoS One. 2016; 11(12):e0167420. PMC: 5132328. DOI: 10.1371/journal.pone.0167420. View

20.

Li R, Hong P, Zheng X . β-carotene attenuates lipopolysaccharide-induced inflammation via inhibition of the NF-κB, JAK2/STAT3 and JNK/p38 MAPK signaling pathways in macrophages. Anim Sci J. 2018; 90(1):140-148. DOI: 10.1111/asj.13108. View