Effect of Antibacterial Root Canal Sealer on Persistent Apical Periodontitis

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2021 Jul 2

PMID 34207470

Citations 7

Authors

Zheng Wang

Ge Yang

Biao Ren

Yuan Gao

Xian Peng

Mingyun Li

Hockin H K Xu

Qi Han

Jiyao Li

Xuedong Zhou

Lei Cheng

Affiliations

Soon will be listed here.

Abstract

The infection of and its interacting microorganisms in the root canal could cause persistent apical periodontitis (AP). Antibacterial root canal sealer has favorable prospects to inhibit biofilms. The purpose of this study was to investigated the antibacterial effect of root canal sealer containing dimethylaminododecyl methacrylate (DMADDM) on persistent AP in beagle dogs for the first time. Persistent AP was established by a two-step infection with and multi-bacteria (, , , ). Root canal sealer containing DMADDM (0%, 1.25%, 2.5%) was used to complete root canal filling. The volume of lesions and inflammatory grade in the apical area were evaluated by cone beam computer tomography (CBCT) and hematoxylin-eosin staining. Both and multi-bacteria-induced persistent AP caused severe apical destruction, and there were no significant differences in pathogenicity between them. DMADDM-modified sealer significantly reduced the volume of periapical lesion and inflammatory grade compared with the control group, among them, the therapeutic effect of the 2.5% group was better than the 1.25% group. In addition, -induced reinfection was more sensitive to the 2.5% group than multi-bacteria reinfection. This study shows that root canal sealer containing DMADDM had a remarkable therapeutic effect on persistent AP, especially on -induced reinfection.

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References

Lin S, Yen-Ping Kuo M, Wang T, Wang J, Yeh F, Hsiao M . Sequential expressions of MMP-1, TIMP-1, IL-6, and COX-2 genes in induced periapical lesions in rats. Eur J Oral Sci. 2002; 110(3):246-53. DOI: 10.1034/j.1600-0447.2002.11227.x. View

Cheng L, Zhang K, Melo M, Weir M, Zhou X, Xu H . Anti-biofilm dentin primer with quaternary ammonium and silver nanoparticles. J Dent Res. 2012; 91(6):598-604. PMC: 3348066. DOI: 10.1177/0022034512444128. View

Liu D, Peng X, Wang S, Han Q, Li B, Zhou X . A novel antibacterial resin-based root canal sealer modified by Dimethylaminododecyl Methacrylate. Sci Rep. 2019; 9(1):10632. PMC: 6650501. DOI: 10.1038/s41598-019-47032-8. View

Del Carpio-Perochena A, Kishen A, Shrestha A, Bramante C . Antibacterial Properties Associated with Chitosan Nanoparticle Treatment on Root Dentin and 2 Types of Endodontic Sealers. J Endod. 2015; 41(8):1353-8. DOI: 10.1016/j.joen.2015.03.020. View

Athanassiadis B, Abbott P, Walsh L . The use of calcium hydroxide, antibiotics and biocides as antimicrobial medicaments in endodontics. Aust Dent J. 2007; 52(1 Suppl):S64-82. DOI: 10.1111/j.1834-7819.2007.tb00527.x. View

Chavez de Paz L . Development of a multispecies biofilm community by four root canal bacteria. J Endod. 2012; 38(3):318-23. DOI: 10.1016/j.joen.2011.11.008. View

Wang S, Ge Y, Zhou X, Xu H, Weir M, Zhang K . Effect of anti-biofilm glass-ionomer cement on Streptococcus mutans biofilms. Int J Oral Sci. 2016; 8(2):76-83. PMC: 4932770. DOI: 10.1038/ijos.2015.55. View

Vengerfeldt V, Spilka K, Saag M, Preem J, Oopkaup K, Truu J . Highly diverse microbiota in dental root canals in cases of apical periodontitis (data of illumina sequencing). J Endod. 2014; 40(11):1778-83. DOI: 10.1016/j.joen.2014.06.017. View

Gao Y, Jiang X, Lin D, Chen Y, Tong Z . The Starvation Resistance and Biofilm Formation of Enterococcus faecalis in Coexistence with Candida albicans, Streptococcus gordonii, Actinomyces viscosus, or Lactobacillus acidophilus. J Endod. 2016; 42(8):1233-8. DOI: 10.1016/j.joen.2016.05.002. View

10.

Kitagawa R, Kitagawa H, Izutani N, Hirose N, Hayashi M, Imazato S . Development of an antibacterial root canal filling system containing MDPB. J Dent Res. 2014; 93(12):1277-82. PMC: 4462803. DOI: 10.1177/0022034514549808. View

11.

Cheng L, Weir M, Zhang K, Arola D, Zhou X, Xu H . Dental primer and adhesive containing a new antibacterial quaternary ammonium monomer dimethylaminododecyl methacrylate. J Dent. 2013; 41(4):345-55. PMC: 3631010. DOI: 10.1016/j.jdent.2013.01.004. View

12.

Cheng L, Weir M, Xu H, Antonucci J, Kraigsley A, Lin N . Antibacterial amorphous calcium phosphate nanocomposites with a quaternary ammonium dimethacrylate and silver nanoparticles. Dent Mater. 2012; 28(5):561-72. PMC: 3322309. DOI: 10.1016/j.dental.2012.01.005. View

13.

Nair P . On the causes of persistent apical periodontitis: a review. Int Endod J. 2006; 39(4):249-81. DOI: 10.1111/j.1365-2591.2006.01099.x. View

14.

Kayaoglu G, Erten H, Bodrumlu E, Orstavik D . The resistance of collagen-associated, planktonic cells of Enterococcus faecalis to calcium hydroxide. J Endod. 2008; 35(1):46-9. DOI: 10.1016/j.joen.2008.09.014. View

15.

Lu B, Zhang J, Huang X, Xiao S, Zhang M, Cai Z . Expression of Interleukin-1β and Matrix Metalloproteinase-8 in Cytolytic and Noncytolytic Enterococcus faecalis-induced Persistent Apical Periodontitis: A Comparative Study in the Rat. J Endod. 2015; 41(8):1288-93. DOI: 10.1016/j.joen.2015.04.019. View

16.

Corotti M, Zambuzzi W, Paiva K, Menezes R, Pinto L, Lara V . Immunolocalization of matrix metalloproteinases-2 and -9 during apical periodontitis development. Arch Oral Biol. 2009; 54(8):764-71. DOI: 10.1016/j.archoralbio.2009.04.013. View

17.

Liang J, Li M, Ren B, Wu T, Xu H, Liu Y . The anti-caries effects of dental adhesive resin influenced by the position of functional groups in quaternary ammonium monomers. Dent Mater. 2017; 34(3):400-411. DOI: 10.1016/j.dental.2017.11.021. View

18.

Liang J, Liu F, Zou J, Xu H, Han Q, Wang Z . pH-Responsive Antibacterial Resin Adhesives for Secondary Caries Inhibition. J Dent Res. 2020; 99(12):1368-1376. DOI: 10.1177/0022034520936639. View

19.

Cheung G, Ho M . Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol. 2001; 16(6):332-7. DOI: 10.1034/j.1399-302x.2001.160603.x. View

20.

Cheng R, Feng Y, Zhang R, Liu W, Lei L, Hu T . The extent of pyroptosis varies in different stages of apical periodontitis. Biochim Biophys Acta Mol Basis Dis. 2017; 1864(1):226-237. DOI: 10.1016/j.bbadis.2017.10.025. View