Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

Overview

Journal J Clin Med

Specialty General Medicine

Date 2021 Jul 2

PMID 34207121

Citations 10

Authors

Kaan Aydos

Oya Sena Aydos

Affiliations

Soon will be listed here.

Abstract

Retrieving spermatozoa from the testicles has been a great hope for patients with non-obstructive azoospermia (NOA), but relevant methods have not yet been developed to the level necessary to provide resolutions for all cases of NOA. Although performing testicular sperm extraction under microscopic magnification has increased sperm retrieval rates, in vitro selection and processing of quality sperm plays an essential role in the success of in vitro fertilization. Moreover, sperm cryopreservation is widely used in assisted reproductive technologies, whether for therapeutic purposes or for future fertility preservation. In recent years, there have been new developments using advanced technologies to freeze and preserve even very small numbers of sperm for which conventional techniques are inadequate. The present review provides an up-to-date summary of current strategies for maximizing sperm recovery from surgically obtained testicular samples and, as an extension, optimization of in vitro sperm processing techniques in the management of NOA.

Citing Articles

Sperm oxidative damage acquired during seminal plasma removal for assisted reproductive technology is reduced by BGP-15.

Gonzalez M, Rose R, Connaughton H, Mackintosh G, Bugeja C, Barry M J Assist Reprod Genet. 2025; .

PMID: 39934465 DOI: 10.1007/s10815-025-03418-4.

Interpretable machine learning models for predicting clinical pregnancies associated with surgical sperm retrieval from testes of different etiologies: a retrospective study.

Cao S, Liu X, Song B, Hu Y BMC Urol. 2024; 24(1):156.

PMID: 39075422 PMC: 11285258. DOI: 10.1186/s12894-024-01537-1.

Application of Intracytoplasmic Morphologically Selected Sperm Injection (IMSI) on a Teratozoospermic Patient and Its Effect on the In-Vitro Fertilization (IVF) Outcome.

Gajabe G, More A, Shrivastava J, Choudhary N, Dutta S, Kadu K Cureus. 2024; 16(1):e53268.

PMID: 38435865 PMC: 10905051. DOI: 10.7759/cureus.53268.

Comparing the pregnancy outcomes of cleavage and blastocyst stage in frozen embryo transfer cycles: A cross-sectional study.

Parsafar Z, Dehghani-Firouzabadi R Int J Reprod Biomed. 2024; 21(11):929-936.

PMID: 38292510 PMC: 10823116. DOI: 10.18502/ijrm.v21i11.14656.

Reviving Spermatogenesis: A Case Report on an Approach to Treat Non-obstructive Azoospermia Using Theophylline, Pentoxifylline, and Hyaluronic Acid.

Pareek C, Badge A, Bawaskar P, More A, Nair N Cureus. 2024; 15(12):e50623.

PMID: 38226124 PMC: 10788697. DOI: 10.7759/cureus.50623.

References

Le M, Nguyen T, Nguyen T, Nguyen V, Nguyen T, Nguyen V . Cryopreservation of human spermatozoa by vitrification versus conventional rapid freezing: Effects on motility, viability, morphology and cellular defects. Eur J Obstet Gynecol Reprod Biol. 2019; 234:14-20. DOI: 10.1016/j.ejogrb.2019.01.001. View

Verma R, Huang Z, Deutschman C, Levy R . Caffeine restores myocardial cytochrome oxidase activity and improves cardiac function during sepsis. Crit Care Med. 2009; 37(4):1397-402. PMC: 3970220. DOI: 10.1097/CCM.0b013e31819cecd6. View

Hsieh Y, Tsai H, Chang C, Lo H . Sperm cryopreservation with empty human or mouse zona pellucidae. A comparison. J Reprod Med. 2000; 45(5):383-6. View

Hosseini A, Khalili M, Talebi A, Agha-Rahimi A, Ghasemi-Esmailabad S, Woodward B . Cryopreservation of Low Number of Human Spermatozoa; Which is Better: Vapor Phase or Direct Submerging in Liquid Nitrogen?. Hum Fertil (Camb). 2018; 22(2):126-132. DOI: 10.1080/14647273.2018.1456681. View

Kim H, Yoon H, Jang J, Oh H, Lee Y, Lee W . Comparison between intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection in oligo-astheno-teratozoospermia patients. Clin Exp Reprod Med. 2014; 41(1):9-14. PMC: 3968258. DOI: 10.5653/cerm.2014.41.1.9. View

De Croo I, Van der Elst J, Everaert K, De Sutter P, Dhont M . Fertilization, pregnancy and embryo implantation rates after ICSI with fresh or frozen-thawed testicular spermatozoa. Hum Reprod. 1998; 13(7):1893-7. DOI: 10.1093/humrep/13.7.1893. View

Du J, Shen J, Wang Y, Pan C, Pang W, Diao H . Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane. Oncotarget. 2016; 7(37):58832-58847. PMC: 5312279. DOI: 10.18632/oncotarget.11315. View

Marh J, Tres L, Yamazaki Y, Yanagimachi R, Kierszenbaum A . Mouse round spermatids developed in vitro from preexisting spermatocytes can produce normal offspring by nuclear injection into in vivo-developed mature oocytes. Biol Reprod. 2003; 69(1):169-76. DOI: 10.1095/biolreprod.102.015099. View

Karacan M, Alwaeely F, Erkan S, Cebi Z, Berberoglugil M, Batukan M . Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia. Fertil Steril. 2013; 100(4):975-80. DOI: 10.1016/j.fertnstert.2013.06.031. View

10.

Stanger J, Vo L, Yovich J, Almahbobi G . Hypo-osmotic swelling test identifies individual spermatozoa with minimal DNA fragmentation. Reprod Biomed Online. 2010; 21(4):474-84. DOI: 10.1016/j.rbmo.2010.06.026. View

11.

Galdon G, Atala A, Sadri-Ardekani H . In Vitro Spermatogenesis: How Far from Clinical Application?. Curr Urol Rep. 2016; 17(7):49. DOI: 10.1007/s11934-016-0605-3. View

12.

Marquez B, Suarez S . Different signaling pathways in bovine sperm regulate capacitation and hyperactivation. Biol Reprod. 2004; 70(6):1626-33. DOI: 10.1095/biolreprod.103.026476. View

13.

Liu Y, Di L, Osterberg E, He L, Li P, Li Z . Use of Raman spectroscopy to identify active spermatogenesis and Sertoli-cell-only tubules in mice. Andrologia. 2016; 48(10):1086-1091. DOI: 10.1111/and.12542. View

14.

Mallidis C, Sanchez V, Wistuba J, Wuebbeling F, Burger M, Fallnich C . Raman microspectroscopy: shining a new light on reproductive medicine. Hum Reprod Update. 2013; 20(3):403-14. DOI: 10.1093/humupd/dmt055. View

15.

Esfandiari N, Javed M, Gotlieb L, Casper R . Complete failed fertilization after intracytoplasmic sperm injection--analysis of 10 years' data. Int J Fertil Womens Med. 2006; 50(4):187-92. View

16.

Del Vento F, Vermeulen M, Ucakar B, Poels J, des Rieux A, Wyns C . Significant Benefits of Nanoparticles Containing a Necrosis Inhibitor on Mice Testicular Tissue Autografts Outcomes. Int J Mol Sci. 2019; 20(23). PMC: 6929043. DOI: 10.3390/ijms20235833. View

17.

Tash J, Means A . Cyclic adenosine 3',5' monophosphate, calcium and protein phosphorylation in flagellar motility. Biol Reprod. 1983; 28(1):75-104. DOI: 10.1095/biolreprod28.1.75. View

18.

Khan I, Cao Z, Liu H, Khan A, Rahman S, Khan M . Impact of Cryopreservation on Spermatozoa Freeze-Thawed Traits and Relevance OMICS to Assess Sperm Cryo-Tolerance in Farm Animals. Front Vet Sci. 2021; 8:609180. PMC: 7947673. DOI: 10.3389/fvets.2021.609180. View

19.

Stanic P, Tandara M, Sonicki Z, Simunic V, Radakovic B, Suchanek E . Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gynecol Reprod Biol. 2000; 91(1):65-70. DOI: 10.1016/s0301-2115(99)00255-9. View

20.

Sousa M, Cremades N, Alves C, Silva J, Barros A . Developmental potential of human spermatogenic cells co-cultured with Sertoli cells. Hum Reprod. 2002; 17(1):161-72. DOI: 10.1093/humrep/17.1.161. View