In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2021 Jul 2

PMID 34206674

Citations 12

Authors

Ionut-Cristian Radu

Catalin Zaharia

Ariana Hudita

Eugenia Tanasa

Octav Ginghina

Minodora Marin

Bianca Galateanu

Marieta Costache

Affiliations

Soon will be listed here.

Abstract

In this paper, silk sericin nanocarriers with a very low size range were obtained by nanoprecipitation. Sericin nanoparticles were loaded with doxorubicin, and they were considered a promising tool for breast cancer therapy. The chemistry, structure, morphology, and size distribution of nanocarriers were investigated by Fourier transformed infrared spectroscopy (FTIR-ATR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and dynamic light scattering (DLS). Morphological investigation and DLS showed the formation of sericin nanoparticles in the 25-40 nm range. FTIR chemical characterization showed specific interactions of protein-doxorubicin-enzymes with a high influence on the drug delivery process and release behavior. The biological investigation via breast cancer cell line revealed a high activity of nanocarriers in cancer cells by inducing significant DNA damage.

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