The Unusual Role of Pro in Cu(II) Binding by His-Cyclopentapeptide

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Jul 2

PMID 34205682

Authors

Aleksandra Pieniezna

Aleksandra Kotynia

Justyna Brasun

Affiliations

Soon will be listed here.

Abstract

In this paper, we present findings from studying the interaction of copper(II) ions with the His-cyclopentapeptide and the role of proline used for the purpose of potentiometric titration and UV-Vis, CD and EPR spectroscopic measurements. Experiments of two homodetic peptides differing by one amino acid residue were conducted for a ligand to metal ratio of 1:1 in the pH range 2.5-11.0. The presented studies reveal that peptides form only mononuclear complexes, and the CuHL complex appears in the system first (for both L1 and L2). Study results show that the presence of Pro influences the structure of formed complexes and their stabilities and has a strong impact on the efficiency of copper(II) coordination.

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