The Stimulating Effect of Rosmarinic Acid and Extracts from Rosemary and Lemon Balm on Collagen Type I Biosynthesis in Osteogenesis Imperfecta Type I Skin Fibroblasts

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2021 Jul 2

PMID 34201872

Citations 5

Authors

Joanna Sutkowska

Natalia Hupert

Katarzyna Gawron

Jakub W Strawa

Michal Tomczyk

Antonella Forlino

Anna Galicka

Affiliations

Soon will be listed here.

Abstract

Rosemary extract (RE) and lemon balm extract (LBE) attract particular attention of pharmacists due to their high therapeutic potential. Osteogenesis imperfecta (OI) type I is a heritable disease caused by mutations in type I collagen and characterized by its reduced amount. The aim of the study was to evaluate the effect of the extracts and rosmarinic acid (RA) on collagen type I level in OI skin fibroblasts. Phytochemical analysis of RE and LBE was carried out by liquid chromatography-photodiode array detection-mass spectrometry. The expression of collagen type I at transcript and protein levels was analyzed by qPCR, ELISA, SDS-urea PAGE, and Western blot. In OI patient's fibroblasts the exposure to the extracts (0.1-100 µg/mL) and RA (0.1-100 µM) significantly increased collagen type I and the best results were obtained with 0.1-10 µM RA and 0.1-10 µg/mL of the extracts. LBE showed a greater stimulating effect than RE, likely due to a higher RA content. Moreover, collagen type III expression and matrix metalloproteinase (MMP-1, -2, -9) activity remained unchanged or decreased. The obtained data support the clinical potential of RA-rich extracts and RA itself in modulating the quantitative defect of type I collagen in type I OI.

Citing Articles

Reversing Glycation with a Dietary Supplement Containing Rosemary Extract.

Draelos Z, Bucay V, Watchmaker J, Valacchi G J Clin Aesthet Dermatol. 2025; 18(2):44-49.

PMID: 40078862 PMC: 11896625.

Optimization of the extraction process for Shenshou Taiyi powder based on Box-Behnken experimental design, standard relation, and FAHP-CRITIC methods.

Jiang M, Qiu Z, Diao Y, Shi Y, Liu W, Li N BMC Complement Med Ther. 2024; 24(1):251.

PMID: 38956573 PMC: 11221082. DOI: 10.1186/s12906-024-04554-7.

Modulatory Effect of Rosmarinic Acid on HO-Induced Adaptive Glycolytic Response in Dermal Fibroblasts.

Charoensin S, Dansakda S Molecules. 2023; 28(14).

PMID: 37513476 PMC: 10384106. DOI: 10.3390/molecules28145599.

Rosemary Extract-Induced Autophagy and Decrease in Accumulation of Collagen Type I in Osteogenesis Imperfecta Skin Fibroblasts.

Sutkowska-Skolimowska J, Branska-Januszewska J, Strawa J, Ostrowska H, Botor M, Gawron K Int J Mol Sci. 2022; 23(18).

PMID: 36142253 PMC: 9499644. DOI: 10.3390/ijms231810341.

Biomedical features and therapeutic potential of rosmarinic acid.

Noor S, Mohammad T, Rub M, Raza A, Azum N, Yadav D Arch Pharm Res. 2022; 45(4):205-228.

PMID: 35391712 PMC: 8989115. DOI: 10.1007/s12272-022-01378-2.

References

Hitl M, Kladar N, Gavaric N, Bozin B . Rosmarinic Acid-Human Pharmacokinetics and Health Benefits. Planta Med. 2020; 87(4):273-282. DOI: 10.1055/a-1301-8648. View

Nazaruk J, Jakoniuk P . Flavonoid composition and antimicrobial activity of Cirsium rivulare (Jacq.) All. flowers. J Ethnopharmacol. 2005; 102(2):208-12. DOI: 10.1016/j.jep.2005.06.012. View

Zhou M, Jiang R, Kim K, Lee J, Kim C, Yin W . Rosmarinic acid inhibits poly(I:C)-induced inflammatory reaction of epidermal keratinocytes. Life Sci. 2016; 155:189-94. DOI: 10.1016/j.lfs.2016.05.023. View

Borras Linares I, Arraez-Roman D, Herrero M, Ibanez E, Segura-Carretero A, Fernandez-Gutierrez A . Comparison of different extraction procedures for the comprehensive characterization of bioactive phenolic compounds in Rosmarinus officinalis by reversed-phase high-performance liquid chromatography with diode array detection coupled to.... J Chromatogr A. 2011; 1218(42):7682-90. DOI: 10.1016/j.chroma.2011.07.021. View

Noguchi-Shinohara M, Ono K, Hamaguchi T, Iwasa K, Nagai T, Kobayashi S . Pharmacokinetics, Safety and Tolerability of Melissa officinalis Extract which Contained Rosmarinic Acid in Healthy Individuals: A Randomized Controlled Trial. PLoS One. 2015; 10(5):e0126422. PMC: 4433273. DOI: 10.1371/journal.pone.0126422. View

Schleit J, Bailey S, Tran T, Chen D, Stowers S, Schwarze U . Molecular Outcome, Prediction, and Clinical Consequences of Splice Variants in COL1A1, Which Encodes the proα1(I) Chains of Type I Procollagen. Hum Mutat. 2015; 36(7):728-39. DOI: 10.1002/humu.22812. View

Bardai G, Lemyre E, Moffatt P, Palomo T, Glorieux F, Tung J . Osteogenesis Imperfecta Type I Caused by COL1A1 Deletions. Calcif Tissue Int. 2015; 98(1):76-84. DOI: 10.1007/s00223-015-0066-6. View

Luo C, Zou L, Sun H, Peng J, Gao C, Bao L . A Review of the Anti-Inflammatory Effects of Rosmarinic Acid on Inflammatory Diseases. Front Pharmacol. 2020; 11:153. PMC: 7059186. DOI: 10.3389/fphar.2020.00153. View

Galicka A, Nazaruk J . Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action. Int J Mol Med. 2007; 20(6):889-95. View

10.

Etich J, Lessmeier L, Rehberg M, Sill H, Zaucke F, Netzer C . Osteogenesis imperfecta-pathophysiology and therapeutic options. Mol Cell Pediatr. 2020; 7(1):9. PMC: 7427672. DOI: 10.1186/s40348-020-00101-9. View

11.

Yui S, Fujiwara S, Harada K, Motoike-Hamura M, Sakai M, Matsubara S . Beneficial Effects of Lemon Balm Leaf Extract on In Vitro Glycation of Proteins, Arterial Stiffness, and Skin Elasticity in Healthy Adults. J Nutr Sci Vitaminol (Tokyo). 2017; 63(1):59-68. DOI: 10.3177/jnsv.63.59. View

12.

Isidorov V, Szoka L, Nazaruk J . Cytotoxicity of white birch bud extracts: Perspectives for therapy of tumours. PLoS One. 2018; 13(8):e0201949. PMC: 6091957. DOI: 10.1371/journal.pone.0201949. View

13.

Willing M, Deschenes S, Scott D, Byers P, Slayton R, Pitts S . Osteogenesis imperfecta type I: molecular heterogeneity for COL1A1 null alleles of type I collagen. Am J Hum Genet. 1994; 55(4):638-47. PMC: 1918287. View

14.

Matwiejczuk N, Galicka A, Brzoska M . Review of the safety of application of cosmetic products containing parabens. J Appl Toxicol. 2020; 40(1):176-210. DOI: 10.1002/jat.3917. View

15.

Sodagam L, Lewinska A, Kwasniewicz E, Kokhanovska S, Wnuk M, Siems K . Phytochemicals Rosmarinic Acid, Ampelopsin, and Amorfrutin-A Can Modulate Age-Related Phenotype of Serially Passaged Human Skin Fibroblasts . Front Genet. 2019; 10:81. PMC: 6394134. DOI: 10.3389/fgene.2019.00081. View

16.

Allegra A, Tonacci A, Pioggia G, Musolino C, Gangemi S . Anticancer Activity of L.: Mechanisms of Action and Therapeutic Potentials. Nutrients. 2020; 12(6). PMC: 7352773. DOI: 10.3390/nu12061739. View

17.

Shakeri A, Sahebkar A, Javadi B . Melissa officinalis L. - A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2016; 188:204-28. DOI: 10.1016/j.jep.2016.05.010. View

18.

Hossain M, Rai D, Brunton N, Martin-Diana A, Barry-Ryan C . Characterization of phenolic composition in Lamiaceae spices by LC-ESI-MS/MS. J Agric Food Chem. 2010; 58(19):10576-81. DOI: 10.1021/jf102042g. View

19.

DOrazio J, Jarrett S, Amaro-Ortiz A, Scott T . UV radiation and the skin. Int J Mol Sci. 2013; 14(6):12222-48. PMC: 3709783. DOI: 10.3390/ijms140612222. View

20.

de Macedo L, Dos Santos E, Militao L, Tundisi L, Ataide J, Souto E . Rosemary ( L., syn Spenn.) and Its Topical Applications: A Review. Plants (Basel). 2020; 9(5). PMC: 7284349. DOI: 10.3390/plants9050651. View