Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2021 Jul 2

PMID 34201002

Citations 7

Authors

Valentina Cocce

Silvia La Monica

Mara Bonelli

Giulio Alessandri

Roberta Alfieri

Costanza Annamaria Lagrasta

Denise Madeddu

Caterina Frati

Lisa Flammini

Daniela Lisini

Angela Marcianti

Eugenio Parati

Francesca Paino

Aldo Gianni

Giampietro Farronato

Angela Falco

Lorenzo Spaggiari

Francesco Petrella

Augusto Pessina

Affiliations

Soon will be listed here.

Abstract

Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM.

Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice.

Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment.

Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy.

Citing Articles

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Cordani N, Lisini D, Cocce V, Paglia G, Meanti R, Cerrito M Int J Mol Sci. 2023; 24(6).

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