Fluorescence Lifetime Changes Induced by Laser Irradiation: A Preclinical Study Towards the Evaluation of Retinal Metabolic States

Overview

Journal Life (Basel)

Specialty Biology

Date 2021 Jul 2

PMID 34199212

Citations 4

Authors

Svenja Rebecca Sonntag

Eric Seifert

Maximilian Hamann

Britta Lewke

Dirk Theisen-Kunde

Salvatore Grisanti

Ralf Brinkmann

Yoko Miura

Affiliations

Soon will be listed here.

Abstract

Fluorescence Lifetime (FLT) of intrinsic fluorophores may alter under the change in metabolic state. In this study, the FLT of rabbit retina was investigated in vivo after laser irradiation using fluorescence lifetime imaging ophthalmoscopy (FLIO). The retina of the Chinchilla bastard rabbits was irradiated with a 514 nm diode laser. FLIO, fundus photography, and optical coherence tomography (OCT) were conducted 30 min and 1 to 3 weeks after treatment. After strong coagulation, the FLT at laser spots was significantly elongated immediately after irradiation, conversely shortened after more than a week. Histological examination showed eosinophilic substance and melanin clumping in subretinal space at the coagulation spots older than one week. The FLT was also elongated right around the coagulation spots, which corresponded to the discontinuous ellipsoid zone (EZ) on OCT. This EZ change was recovered after one week, and the FLT became the same level as the surroundings. In addition, there was a region around the laser spot where the FLT was temporarily shorter than the surrounding area. When weak pulse energy was applied to selectively destroy only the RPE, a shortening of the FLT was observed immediately around the laser spot within one week after irradiation. FLIO could serve as a tool to evaluate the structural and metabolic response of the retina to laser treatments.

Citing Articles

Detection sensitivity of fluorescence lifetime imaging ophthalmoscopy for laser-induced selective damage of retinal pigment epithelium.

Sonntag S, Hamann M, Seifert E, Grisanti S, Brinkmann R, Miura Y Graefes Arch Clin Exp Ophthalmol. 2024; 262(9):2885-2895.

PMID: 38587656 PMC: 11377681. DOI: 10.1007/s00417-024-06449-2.

Artificial Intelligence in Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Data Analysis-Toward Retinal Metabolic Diagnostics.

Thiemann N, Sonntag S, Kreikenbohm M, Bohmerle G, Stagge J, Grisanti S Diagnostics (Basel). 2024; 14(4).

PMID: 38396470 PMC: 10888399. DOI: 10.3390/diagnostics14040431.

Impact of cigarette smoking on fluorescence lifetime of ocular fundus.

Sonntag S, Kreikenbohm M, Bohmerle G, Stagge J, Grisanti S, Miura Y Sci Rep. 2023; 13(1):11484.

PMID: 37460627 PMC: 10352358. DOI: 10.1038/s41598-023-37484-4.

Retinal Disease and Metabolism.

Fu Z, Usui-Ouchi A, Allen W, Tomita Y Life (Basel). 2022; 12(2).

PMID: 35207471 PMC: 8879522. DOI: 10.3390/life12020183.

References

Sauer L, Vitale A, Andersen K, Hart B, Bernstein P . FLUORESCENCE LIFETIME IMAGING OPHTHALMOSCOPY (FLIO) PATTERNS IN CLINICALLY UNAFFECTED CHILDREN OF MACULAR TELANGIECTASIA TYPE 2 (MACTEL) PATIENTS. Retina. 2019; 40(4):695-704. PMC: 7062574. DOI: 10.1097/IAE.0000000000002646. View

Brinkmann R, Roider J, Birngruber R . Selective retina therapy (SRT): a review on methods, techniques, preclinical and first clinical results. Bull Soc Belge Ophtalmol. 2007; (302):51-69. View

Dysli C, Wolf S, Hatz K, Zinkernagel M . Fluorescence Lifetime Imaging in Stargardt Disease: Potential Marker for Disease Progression. Invest Ophthalmol Vis Sci. 2016; 57(3):832-41. DOI: 10.1167/iovs.15-18033. View

Sparrow J, Wu Y, Nagasaki T, Yoon K, Yamamoto K, Zhou J . Fundus autofluorescence and the bisretinoids of retina. Photochem Photobiol Sci. 2010; 9(11):1480-9. PMC: 4071605. DOI: 10.1039/c0pp00207k. View

Schuele G, Rumohr M, Huettmann G, Brinkmann R . RPE damage thresholds and mechanisms for laser exposure in the microsecond-to-millisecond time regimen. Invest Ophthalmol Vis Sci. 2005; 46(2):714-9. DOI: 10.1167/iovs.04-0136. View

Framme C, Schule G, Roider J, Birngruber R, Brinkmann R . Online autofluorescence measurements during selective RPE laser treatment. Graefes Arch Clin Exp Ophthalmol. 2004; 242(10):863-9. DOI: 10.1007/s00417-004-0938-3. View

Sauer L, Gensure R, Andersen K, Kreilkamp L, Hageman G, Hammer M . Patterns of Fundus Autofluorescence Lifetimes In Eyes of Individuals With Nonexudative Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci. 2018; 59(4):AMD65-AMD77. PMC: 6009207. DOI: 10.1167/iovs.17-23764. View

Sauer L, Andersen K, Dysli C, Zinkernagel M, Bernstein P, Hammer M . Review of clinical approaches in fluorescence lifetime imaging ophthalmoscopy. J Biomed Opt. 2018; 23(9):1-20. PMC: 8357196. DOI: 10.1117/1.JBO.23.9.091415. View

Seifert E, Sonntag S, Kleingarn P, Theisen-Kunde D, Grisanti S, Birngruber R . Investigations on Retinal Pigment Epithelial Damage at Laser Irradiation in the Lower Microsecond Time Regime. Invest Ophthalmol Vis Sci. 2021; 62(3):32. PMC: 7991964. DOI: 10.1167/iovs.62.3.32. View

10.

Alam S, Wallrabe H, Svindrych Z, Chaudhary A, Christopher K, Chandra D . Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay. Sci Rep. 2017; 7(1):10451. PMC: 5585313. DOI: 10.1038/s41598-017-10856-3. View

11.

Gawecki M, Jaszczuk-Maciejewska A, Jurska-Jasko A, Kneba M, Grzybowski A . Transfoveal Micropulse Laser Treatment of Central Serous Chorioretinopathy within Six Months of Disease Onset. J Clin Med. 2019; 8(9). PMC: 6780961. DOI: 10.3390/jcm8091398. View

12.

Sauer L, Gensure R, Hammer M, Bernstein P . Fluorescence Lifetime Imaging Ophthalmoscopy: A Novel Way to Assess Macular Telangiectasia Type 2. Ophthalmol Retina. 2018; 2(6):587-598. PMC: 6089530. DOI: 10.1016/j.oret.2017.10.008. View

13.

Schweitzer D, Deutsch L, Klemm M, Jentsch S, Hammer M, Peters S . Fluorescence lifetime imaging ophthalmoscopy in type 2 diabetic patients who have no signs of diabetic retinopathy. J Biomed Opt. 2015; 20(6):61106. DOI: 10.1117/1.JBO.20.6.061106. View

14.

Stefansson E, Hatchell D, Fisher B, Sutherland F, MACHEMER R . Panretinal photocoagulation and retinal oxygenation in normal and diabetic cats. Am J Ophthalmol. 1986; 101(6):657-64. DOI: 10.1016/0002-9394(86)90765-8. View

15.

Koinzer S, Saeger M, Hesse C, Portz L, Kleemann S, Schlott K . Correlation with OCT and histology of photocoagulation lesions in patients and rabbits. Acta Ophthalmol. 2013; 91(8):e603-11. DOI: 10.1111/aos.12188. View

16.

Inagaki K, Hamada M, Ohkoshi K . Minimally invasive laser treatment combined with intravitreal injection of anti-vascular endothelial growth factor for diabetic macular oedema. Sci Rep. 2019; 9(1):7585. PMC: 6527558. DOI: 10.1038/s41598-019-44130-5. View

17.

Luttrull J, Musch D, Mainster M . Subthreshold diode micropulse photocoagulation for the treatment of clinically significant diabetic macular oedema. Br J Ophthalmol. 2004; 89(1):74-80. PMC: 1772486. DOI: 10.1136/bjo.2004.051540. View

18.

Elsner H, Porksen E, Klatt C, Bunse A, Theisen-Kunde D, Brinkmann R . Selective retina therapy in patients with central serous chorioretinopathy. Graefes Arch Clin Exp Ophthalmol. 2006; 244(12):1638-45. DOI: 10.1007/s00417-006-0368-5. View

19.

Dysli C, Wolf S, Zinkernagel M . Fluorescence lifetime imaging in retinal artery occlusion. Invest Ophthalmol Vis Sci. 2015; 56(5):3329-36. DOI: 10.1167/iovs.14-16203. View

20.

Walsh A, Cook R, Manning H, Hicks D, Lafontant A, Arteaga C . Optical metabolic imaging identifies glycolytic levels, subtypes, and early-treatment response in breast cancer. Cancer Res. 2013; 73(20):6164-74. PMC: 3801432. DOI: 10.1158/0008-5472.CAN-13-0527. View