Long Noncoding RNA WDFY3-AS2 Represses the Progression of Esophageal Cancer Through MiR-18a/PTEN Axis

Overview

Journal J Oncol

Specialty Oncology

Date 2021 Jul 1

PMID 34194502

Citations 6

Authors

Qingling Kong

Guangcai Li

Gang Yin

Kun Li

Dongqing Zhang

Weihao Xu

Affiliations

Soon will be listed here.

Abstract

Background: Understanding the role of lncRNAs in the development of human malignancies is necessary for the targeted therapy of malignant tumors, including esophageal cancer (EC). Nevertheless, the specific role and regulatory mechanism of lncRNA WDFY3-AS2 in EC are still unclear. Here, we examined the functional role and regulatory mechanism of WDFY3-AS2 in EC.

Materials And Methods: RT-qPCR assay was applied to measure the expression of WDFY3-AS2 and miR-18a in EC samples and cells. The luciferase reporter and RIP assays were used to check the relationship between WDFY3-AS2, miR-18a, and PTEN. Counting Clock Kit-8 (CCK-8) assay was carried out to detect cell viability, and transwell assays were used for measuring cell migration and invasion.

Results: Underexpression of WDFY3-AS2 was found in EC specimens and cells, which predicted a poor prognosis of EC patients. Reexpression of WDFY3-AS2 repressed the progression of EC via inhibiting cell proliferation, migration, and invasion. Additionally, WDFY3-AS2 was negatively correlated with miR-18a and positively with PTEN. Furthermore, we discovered that the expression of PTEN decreased by miR-18a mimic was rescued by WDFY3-AS2 overexpression.

Conclusions: WDFY3-AS2 modulates the expressional level of PTEN as a competitive endogenous RNA via sponging miR-18a in EC, which suggests that the WDFY3-AS2/miR-18a/PTEN pathway might be involved in the progression of EC.

Citing Articles

Identification and Verification of an Alternative Polyadenylation-Related lncRNA Prognostic Signature for Glioma.

Wang H, Jiang Z Comput Math Methods Med. 2024; 2022:2164229.

PMID: 39279987 PMC: 11401696. DOI: 10.1155/2022/2164229.

Knockdown of LINC01138 protects human chondrocytes against IL-1β-induced damage by regulating the hsa-miR-1207-5p/KIAA0101 axis.

Zhang J, Lv G Immun Inflamm Dis. 2023; 11(1):e744.

PMID: 36705420 PMC: 9753829. DOI: 10.1002/iid3.744.

Tumor cell-derived exosomal lncRNA LOC441178 inhibits the tumorigenesis of esophageal carcinoma through suppressing macrophage M2 polarization.

Chen J, Chen Z, Hu W, Cai D Eur J Histochem. 2022; 66(4).

PMID: 36250676 PMC: 9627538. DOI: 10.4081/ejh.2022.3510.

Evaluate the immune-related eRNA models and signature score to predict the response to immunotherapy in thyroid carcinoma.

Wu P, Shi J, Wang Z, Sun W, Zhang H Cancer Cell Int. 2022; 22(1):307.

PMID: 36217201 PMC: 9549686. DOI: 10.1186/s12935-022-02722-8.

The prognostic value and immune landscape of a cuproptosis-related lncRNA signature in head and neck squamous cell carcinoma.

Li Y, Li H, Zhang Q, Wei S Front Genet. 2022; 13:942785.

PMID: 35942287 PMC: 9356288. DOI: 10.3389/fgene.2022.942785.

References

Nair M, Prabhu J, Korlimarla A, Rajarajan S, P S H, Kaul R . miR-18a activates Wnt pathway in ER-positive breast cancer and is associated with poor prognosis. Cancer Med. 2020; 9(15):5587-5597. PMC: 7402845. DOI: 10.1002/cam4.3183. View

Jamali L, Tofigh R, Tutunchi S, Panahi G, Borhani F, Akhavan S . Circulating microRNAs as diagnostic and therapeutic biomarkers in gastric and esophageal cancers. J Cell Physiol. 2018; 233(11):8538-8550. DOI: 10.1002/jcp.26850. View

Hirajima S, Komatsu S, Ichikawa D, Takeshita H, Konishi H, Shiozaki A . Clinical impact of circulating miR-18a in plasma of patients with oesophageal squamous cell carcinoma. Br J Cancer. 2013; 108(9):1822-9. PMC: 3658511. DOI: 10.1038/bjc.2013.148. View

Li W, Ma S, Bai X, Pan W, Ai L, Tan W . Long noncoding RNA WDFY3-AS2 suppresses tumor progression by acting as a competing endogenous RNA of microRNA-18a in ovarian cancer. J Cell Physiol. 2019; 235(2):1141-1154. DOI: 10.1002/jcp.29028. View

Mai S, Xiao R, Shi L, Zhou X, Yang T, Zhang M . MicroRNA-18a promotes cancer progression through SMG1 suppression and mTOR pathway activation in nasopharyngeal carcinoma. Cell Death Dis. 2019; 10(11):819. PMC: 6817863. DOI: 10.1038/s41419-019-2060-9. View

Liu H, Zhang Q, Lou Q, Zhang X, Cui Y, Wang P . Differential Analysis of lncRNA, miRNA and mRNA Expression Profiles and the Prognostic Value of lncRNA in Esophageal Cancer. Pathol Oncol Res. 2019; 26(2):1029-1039. DOI: 10.1007/s12253-019-00655-8. View

Chen L, Dzakah E, Shan G . Targetable long non-coding RNAs in cancer treatments. Cancer Lett. 2018; 418:119-124. DOI: 10.1016/j.canlet.2018.01.042. View

Shen S, Li K, Liu Y, Liu X, Liu B, Ba Y . Silencing lncRNA AGAP2-AS1 Upregulates miR-195-5p to Repress Migration and Invasion of EC Cells via the Decrease of FOSL1 Expression. Mol Ther Nucleic Acids. 2020; 20:331-344. PMC: 7082499. DOI: 10.1016/j.omtn.2019.12.036. View

Hemming M, Heinrich M, Bauer S, George S . Translational insights into gastrointestinal stromal tumor and current clinical advances. Ann Oncol. 2018; 29(10):2037-2045. DOI: 10.1093/annonc/mdy309. View

10.

Zhang Y . Epidemiology of esophageal cancer. World J Gastroenterol. 2013; 19(34):5598-606. PMC: 3769895. DOI: 10.3748/wjg.v19.i34.5598. View

11.

Zhang Z, Li J, Wei Z, Li F, Li H, Xiao Y . Correlation between expression levels of lncRNA UCA1 and miR-18a with prognosis of hepatocellular cancer. Eur Rev Med Pharmacol Sci. 2020; 24(7):3586-3591. DOI: 10.26355/eurrev_202004_20820. View

12.

Xiang C, Zhang Y, Zhang Y, Liu C, Hou Y, Zhang Y . lncRNA LEF1-AS1 Promotes Proliferation and Induces Apoptosis of Non-Small-Cell Lung Cancer Cells by Regulating miR-221/PTEN Signaling. Cancer Manag Res. 2020; 12:3845-3850. PMC: 7260488. DOI: 10.2147/CMAR.S246422. View

13.

Liang H, Fan J, Qiao Y . Epidemiology, etiology, and prevention of esophageal squamous cell carcinoma in China. Cancer Biol Med. 2017; 14(1):33-41. PMC: 5365188. DOI: 10.20892/j.issn.2095-3941.2016.0093. View

14.

Zhang Z, Wang S, Liu W . EMT-related long non-coding RNA in hepatocellular carcinoma: A study with TCGA database. Biochem Biophys Res Commun. 2018; 503(3):1530-1536. DOI: 10.1016/j.bbrc.2018.07.075. View

15.

Liang X, Wu Z, Shen S, Niu Y, Guo Y, Liang J . LINC01980 facilitates esophageal squamous cell carcinoma progression via regulation of miR-190a-5p/MYO5A pathway. Arch Biochem Biophys. 2020; 686:108371. DOI: 10.1016/j.abb.2020.108371. View

16.

Xie Y, Cheng Y . LINC01198 facilitates gliomagenesis through activating PI3K/AKT pathway. RNA Biol. 2020; 17(7):1040-1052. PMC: 7549703. DOI: 10.1080/15476286.2020.1755112. View

17.

Jiang C, Li S, Li Y, Bai Y . Anticancer Effects of Dihydroartemisinin on Human Esophageal Cancer Cells In Vivo. Anal Cell Pathol (Amst). 2018; 2018:8759745. PMC: 5985077. DOI: 10.1155/2018/8759745. View

18.

Codipilly D, Qin Y, Dawsey S, Kisiel J, Topazian M, Ahlquist D . Screening for esophageal squamous cell carcinoma: recent advances. Gastrointest Endosc. 2018; 88(3):413-426. PMC: 7493990. DOI: 10.1016/j.gie.2018.04.2352. View

19.

Bach D, Lee S . Long noncoding RNAs in cancer cells. Cancer Lett. 2018; 419:152-166. DOI: 10.1016/j.canlet.2018.01.053. View

20.

Li Q, Dai Z, Xia C, Jin L, Chen X . Suppression of long non-coding RNA MALAT1 inhibits survival and metastasis of esophagus cancer cells by sponging miR-1-3p/CORO1C/TPM3 axis. Mol Cell Biochem. 2020; 470(1-2):165-174. DOI: 10.1007/s11010-020-03759-x. View