Androgenic Sensitivities and Ovarian Gene Expression Profiles Prior to Treatment in Japanese Eel (Anguilla Japonica)

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2021 Jun 30

PMID 34191211

Citations 1

Authors

Yung-Sen Huang

Wen-Chih Cheng

Chung-Yen Lin

Affiliations

Soon will be listed here.

Abstract

Androgens stimulate ovarian development in eels. Our previous report indicated a correlation between the initial (debut) ovarian status (determined by kernel density estimation (KDE), presented as a probability density of oocyte size) and the consequence of 17MT treatment (change in ovary). The initial ovarian status appeared to be an important factor influencing ovarian androgenic sensitivity. We postulated that the sensitivities of initial ovaries are correlated with their gene expression profiles. Japanese eels underwent operation to sample the initial ovarian tissues, and the samples were stored in liquid nitrogen. Using high-throughput next-generation sequencing (NGS) technology, ovarian transcriptomic data were mined and analyzed based on functional gene classification with cutoff-based differentially expressed genes (DEGs); the ovarian status was transformed into gene expression profiles globally or was represented by a set of gene list. Our results also implied that the initial ovary might be an important factor influencing the outcomes of 17MT treatments, and the genes related with neuronal activities or neurogenesis seemed to play an essential role in the positive effect.

Citing Articles

Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Hsu H, Chuang C, Lu I, Lin C, Han Y Zool Stud. 2023; 62:e2.

PMID: 37124871 PMC: 10131072. DOI: 10.6620/ZS.2023.62-02.

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