The Recombinant Plant Bauhinia Bauhinioides Elastase Inhibitor Reduces Rat Thrombus Without Alterations in Hemostatic Parameters

Overview

Journal Sci Rep

Specialty Science

Date 2021 Jun 30

PMID 34188079

Citations 2

Authors

Cleide Oliveira

Mayara Vioto Valois

Tatiana Fontes Ottaiano

Antonio Miranda

Daiane Hansen

Misako Uemura Sampaio

Maria Luiza Vilela Oliva

Francisco Humberto de Abreu Maffei

Affiliations

Soon will be listed here.

Abstract

The anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His, heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His (1.40 mg/kg), with the rBbCI-His mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His alone had an antithrombotic effect, not altering blood clotting or bleeding time.

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