CypB Promotes Cell Proliferation and Metastasis in Endometrial Carcinoma

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2021 Jun 30

PMID 34187415

Citations 3

Authors

Jing Liu

Ying Zuo

Gui-Mei Qu

Xiao Song

Zhong-Hui Liu

Ting-Guo Zhang

Zhu-Hua Zheng

Hong-Kun Wang

Affiliations

Soon will be listed here.

Abstract

Background: The molecular pathogenesis of endometrial cancer is not completely understood. CypB upregulated in many cancers, however, its role in endometrial carcinoma has not been studied. Here, we determine the effect of CypB on the growth of endometrial cancer.

Methods: In this study, we examined the expression of CypB in endometrial cancer tissues using immunohistochemistry. CypB silenced in HEC-1-B cell line by shRNA. CCK-8, colony formation assays, wound healing assays, and transwell analysis were performed to assess its effect on tumor cell proliferation and metastasis. Furthermore, microarray analysis was carried out to compare the global mRNA expression profile between the HEC-1-B and CypB-silenced HEC-1-B cells. Gene ontology and KEGG pathway enrichment analysis were performed to determine the potential function of differentially expressed genes related to CypB.

Results: We found that CypB was upregulated in endometrial cancer, inhibit CypB expression could significantly suppress cell proliferation, metastasis, and migration. We identified 1536 differentially expressed genes related to CypB (onefold change, p < 0.05), among which 652 genes were upregulated and 884 genes were downregulated. The genes with significant difference in top were mainly enriched in the cell cycle, glycosphingolipid biosynthesis, adherens junctions, and metabolism pathways.

Conclusion: The results of our study suggest that CypB may serve as a novel regulator of endometrial cell proliferation and metastasis, thus representing a novel target for gene-targeted endometrial therapy.

Trial Registration: YLYLLS [2018] 008. Registered 27 November 2017.

Citing Articles

The PPIase Activity of CypB Is Essential for the Activation of Both AKT/mTOR and XBP1s Signaling Pathways during the Differentiation of 3T3-L1 Preadipocytes.

Kim G, Yoon K, Ha J, Kang I, Choe W Nutrients. 2024; 16(15).

PMID: 39125345 PMC: 11313753. DOI: 10.3390/nu16152465.

PPIB/Cyclophilin B expression associates with tumor progression and unfavorable survival in patients with pulmonary adenocarcinoma.

Hwang I, Song J, Cho E, Song K, Ra S, Choi C Am J Cancer Res. 2024; 14(2):917-930.

PMID: 38455410 PMC: 10915315.

PPIB-regulated alternative splicing of cell cycle genes contributes to the regulation of cell proliferation.

Zhang Y, Liu L, Zhou M, Zhang Y, Su H, Dong D Am J Transl Res. 2022; 14(9):6163-6174.

PMID: 36247241 PMC: 9556483.

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