β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

Overview

Journal Front Bioeng Biotechnol

Date 2021 Jun 28

PMID 34178968

Citations 1

Authors

Anastasia Zerva

Koar Chorozian

Anastasia S Kritikou

Nikolaos S Thomaidis

Evangelos Topakas

Affiliations

Soon will be listed here.

Abstract

Bertoni is a plant cultivated worldwide due to its use as a sweetener. The sweet taste of stevia is attributed to its numerous steviol glycosides, however, their use is still limited, due to their bitter aftertaste. The transglycosylation of steviol glycosides, aiming at the improvement of their taste, has been reported for many enzymes, however, glycosyl hydrolases are not extensively studied in this respect. In the present study, a β-glucosidase, Bgl3a, and a β-galactosidase, bGal1, have been applied in the transglycosylation of two steviol glycosides, stevioside and rebaudioside A. The maximum conversion yields were 34.6 and 33.1% for stevioside, while 25.6 and 37.6% were obtained for rebaudioside A conversion by Bgl3a and bGal1, respectively. Low-cost industrial byproducts were employed as sugar donors, such as cellulose hydrolyzate and acid whey for bGal1- and Bgl3a- mediated bioconversion, respectively. LC-HRMS analysis identified the formation of mono- and di- glycosylated products from stevioside and rebaudioside A. Overall, the results of the present work indicate that both biocatalysts can be exploited for the design of a cost-effective process for the modification of steviol glycosides.

Citing Articles

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors.

Zerva A, Mohammadi M, Dimopoulos G, Taoukis P, Topakas E Waste Biomass Valorization. 2023; :1-11.

PMID: 36713934 PMC: 9872074. DOI: 10.1007/s12649-023-02052-4.

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