3-Phenyllactic Acid, a Root-promoting Substance Isolated from Bokashi Fertilizer, Exhibits Synergistic Effects with Tryptophan

Overview

Journal Plant Biotechnol (Tokyo)

Date 2021 Jun 28

PMID 34177319

Citations 8

Authors

Yuko Maki

Hiroshi Soejima

Toru Kitamura

Tamizi Sugiyama

Takeo Sato

Masaaki K Watahiki

Junji Yamaguchi

Affiliations

Soon will be listed here.

Abstract

Bokashi fertilizer, an organic fertilizer made of plant residue, has been used in Japan not only to fertilize plants but to regulate their growth. Lactic acid bacteria have been found to play an important role in the fermentation process of Bokashi, but the relationship between these bacteria and plant growth activity has not been clarified. Using the adzuki rooting assay, this study identified 3-phenyllactic acid (PLA) produced by lactic acid bacteria as a root promoting compound in Bokashi. PLA showed synergistic effect with tryptophan, but no stem elongation activity. Lactic acid bacteria produced equal quantities of the L- and D-forms of PLA, which have similar root promoting activity. PLA did not significantly affect the amount of endogenous indole-3-acetic acid (IAA), although the chemical structure of PLA is highly similar to that of L-2-aminooxy-3-phenypropionic acid (L-AOPP), which inhibits IAA biosynthesis. These results indicate that the root promoting activity of PLA is not simply due to its increase in the amount of active auxin.

Citing Articles

Unlocking the potential of ecofriendly guardians for biological control of plant diseases, crop protection and production in sustainable agriculture.

Malik D, Kumar S, Sindhu S 3 Biotech. 2025; 15(4):82.

PMID: 40071128 PMC: 11891127. DOI: 10.1007/s13205-025-04243-3.

A microbiota-derived metabolite, 3-phenyllactic acid, prolongs healthspan by enhancing mitochondrial function and stress resilience via SKN-1/ATFS-1 in C. elegans.

Kim J, Jo Y, Lim G, Ji Y, Roh J, Kim W Nat Commun. 2024; 15(1):10773.

PMID: 39737960 PMC: 11686233. DOI: 10.1038/s41467-024-55015-1.

AcidAGE: a biological age determination neural network based on urine organic acids.

Kobelyatskaya A, Isaev F, Kudryavtseva A, Guvatova Z, Moskalev A Biogerontology. 2024; 26(1):20.

PMID: 39643761 DOI: 10.1007/s10522-024-10161-3.

Lactic acid bacteria: A sustainable solution against phytopathogenic agents.

Saragoca A, Canha H, Varanda C, Materatski P, Cordeiro A, Gama J Environ Microbiol Rep. 2024; 16(6):e70021.

PMID: 39623703 PMC: 11611765. DOI: 10.1111/1758-2229.70021.

The potential of lactic acid bacteria in mediating the control of plant diseases and plant growth stimulation in crop production - A mini review.

Jaffar N, Jawan R, Chong K Front Plant Sci. 2023; 13:1047945.

PMID: 36714743 PMC: 9880282. DOI: 10.3389/fpls.2022.1047945.

References

Drew M, Jackson M, Giffard S . Ethylene-promoted adventitious rooting and development of cortical air spaces (aerenchyma) in roots may be adaptive responses to flooding in Zea mays L. Planta. 2013; 147(1):83-8. DOI: 10.1007/BF00384595. View

Staswick P, Tiryaki I . The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis. Plant Cell. 2004; 16(8):2117-27. PMC: 519202. DOI: 10.1105/tpc.104.023549. View

Hilbert M, Voll L, Ding Y, Hofmann J, Sharma M, Zuccaro A . Indole derivative production by the root endophyte Piriformospora indica is not required for growth promotion but for biotrophic colonization of barley roots. New Phytol. 2012; 196(2):520-534. DOI: 10.1111/j.1469-8137.2012.04275.x. View

Peret B, De Rybel B, Casimiro I, Benkova E, Swarup R, Laplaze L . Arabidopsis lateral root development: an emerging story. Trends Plant Sci. 2009; 14(7):399-408. DOI: 10.1016/j.tplants.2009.05.002. View

Xu L, Geelen D . Developing Biostimulants From Agro-Food and Industrial By-Products. Front Plant Sci. 2018; 9:1567. PMC: 6218572. DOI: 10.3389/fpls.2018.01567. View

Hirata H, Ohnishi T, Watanabe N . Biosynthesis of floral scent 2-phenylethanol in rose flowers. Biosci Biotechnol Biochem. 2016; 80(10):1865-73. DOI: 10.1080/09168451.2016.1191333. View

Cortes-Zavaleta O, Lopez-Malo A, Hernandez-Mendoza A, Garcia H . Antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production. Int J Food Microbiol. 2014; 173:30-5. DOI: 10.1016/j.ijfoodmicro.2013.12.016. View

Soeno K, Goda H, Ishii T, Ogura T, Tachikawa T, Sasaki E . Auxin biosynthesis inhibitors, identified by a genomics-based approach, provide insights into auxin biosynthesis. Plant Cell Physiol. 2010; 51(4):524-36. DOI: 10.1093/pcp/pcq032. View

Prasuna M, Mujahid M, Sasikala C, Ramana C . L-Phenylalanine catabolism and L-phenyllactic acid production by a phototrophic bacterium, Rubrivivax benzoatilyticus JA2. Microbiol Res. 2012; 167(9):526-31. DOI: 10.1016/j.micres.2012.03.001. View

10.

Nikitin A, Cheshyk I, Gutseva G, Tankevich E, Shintani M, Okumoto S . Impact of effective microorganisms on the transfer of radioactive cesium into lettuce and barley biomass. J Environ Radioact. 2018; 192:491-497. DOI: 10.1016/j.jenvrad.2018.08.005. View

11.

Yakhin O, Lubyanov A, Yakhin I, Brown P . Biostimulants in Plant Science: A Global Perspective. Front Plant Sci. 2017; 7:2049. PMC: 5266735. DOI: 10.3389/fpls.2016.02049. View

12.

Vermeulen N, Ganzle M, Vogel R . Influence of peptide supply and cosubstrates on phenylalanine metabolism of Lactobacillus sanfranciscensis DSM20451(T) and Lactobacillus plantarum TMW1.468. J Agric Food Chem. 2006; 54(11):3832-9. DOI: 10.1021/jf052733e. View

13.

Xu D, Miao J, Yumoto E, Yokota T, Asahina M, Watahiki M . YUCCA9-Mediated Auxin Biosynthesis and Polar Auxin Transport Synergistically Regulate Regeneration of Root Systems Following Root Cutting. Plant Cell Physiol. 2017; 58(10):1710-1723. PMC: 5921505. DOI: 10.1093/pcp/pcx107. View

14.

Stepanova A, Robertson-Hoyt J, Yun J, Benavente L, Xie D, Dolezal K . TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development. Cell. 2008; 133(1):177-91. DOI: 10.1016/j.cell.2008.01.047. View

15.

Jaramillo-Lopez P, Ramirez M, Perez-Salicrup D . Impacts of Bokashi on survival and growth rates of Pinus pseudostrobus in community reforestation projects. J Environ Manage. 2014; 150:48-56. DOI: 10.1016/j.jenvman.2014.11.003. View

16.

Tao Y, Ferrer J, Ljung K, Pojer F, Hong F, Long J . Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants. Cell. 2008; 133(1):164-76. PMC: 2442466. DOI: 10.1016/j.cell.2008.01.049. View

17.

Tian C, Muto H, Higuchi K, Matamura T, Tatematsu K, Koshiba T . Disruption and overexpression of auxin response factor 8 gene of Arabidopsis affect hypocotyl elongation and root growth habit, indicating its possible involvement in auxin homeostasis in light condition. Plant J. 2004; 40(3):333-43. DOI: 10.1111/j.1365-313X.2004.02220.x. View

18.

Fonseca S, Chini A, Hamberg M, Adie B, Porzel A, Kramell R . (+)-7-iso-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate. Nat Chem Biol. 2009; 5(5):344-50. DOI: 10.1038/nchembio.161. View

19.

Lieberman M, Kunishi A . Ethylene production from methionine. Biochem J. 1965; 97(2):449-59. PMC: 1264661. DOI: 10.1042/bj0970449. View

20.

Mu W, Yu S, Zhu L, Zhang T, Jiang B . Recent research on 3-phenyllactic acid, a broad-spectrum antimicrobial compound. Appl Microbiol Biotechnol. 2012; 95(5):1155-63. DOI: 10.1007/s00253-012-4269-8. View