High Resolution Mapping and Candidate Gene Identification of Downy Mildew Race 16 Resistance in Spinach

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2021 Jun 27

PMID 34174825

Citations 9

Authors

Gehendra Bhattarai

Wei Yang

Ainong Shi

Chunda Feng

Braham Dhillon

James C Correll

Beiquan Mou

Affiliations

Soon will be listed here.

Abstract

Background: Downy mildew, the most devastating disease of spinach (Spinacia oleracea L.), is caused by the oomycete Peronospora effusa [=P. farinosa f. sp. spinaciae]. The P. effusa shows race specificities to the resistant host and comprises 19 reported races and many novel isolates. Sixteen new P. effusa races were identified during the past three decades, and the new pathogen races are continually overcoming the genetic resistances used in commercial cultivars. A spinach breeding population derived from the cross between cultivars Whale and Lazio was inoculated with P. effusa race 16 in an environment-controlled facility; disease response was recorded and genotyped using genotyping by sequencing (GBS). The main objective of this study was to identify resistance-associated single nucleotide polymorphism (SNP) markers from the cultivar Whale against the P. effusa race 16.

Results: Association analysis conducted using GBS markers identified six significant SNPs (S3_658,306, S3_692697, S3_1050601, S3_1227787, S3_1227802, S3_1231197). The downy mildew resistance locus from cultivar Whale was mapped to a 0.57 Mb region on chromosome 3, including four disease resistance candidate genes (Spo12736, Spo12784, Spo12908, and Spo12821) within 2.69-11.28 Kb of the peak SNP.

Conclusions: Genomewide association analysis approach was used to map the P. effusa race 16 resistance loci and identify associated SNP markers and the candidate genes. The results from this study could be valuable in understanding the genetic basis of downy mildew resistance, and the SNP marker will be useful in spinach breeding to select resistant lines.

Citing Articles

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Bhattarai K, Ogden A, Pandey S, Sandoya G, Shi A, Nankar A Front Plant Sci. 2025; 15:1524601.

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Dual transcriptional characterization of spinach and Peronospora effusa during resistant and susceptible race-cultivar interactions.

Clark K, Feng C, Anchieta A, Van Deynze A, Correll J, Klosterman S BMC Genomics. 2024; 25(1):937.

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A Genome-Wide Association Study Reveals the Genetic Mechanisms of Nutrient Accumulation in Spinach.

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Skim resequencing finely maps the downy mildew resistance loci and in spinach cultivars whale and Lazio.

Bhattarai G, Shi A, Mou B, Correll J Hortic Res. 2023; 10(6):uhad076.

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Two decades of association mapping: Insights on disease resistance in major crops.

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