MiR-335 Targets CRIM1 to Promote the Proliferation and Inhibit the Apoptosis of Placental Trophoblast Cells in Preeclamptic Rats

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2021 Jun 21

PMID 34150047

Citations 2

Authors

Liuyan Jiang

Meiyan Li

Haisi Gan

Li Yang

Fengjuan Lin

Min Hu

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the role of miRNA-335 on the proliferation and apoptosis of placental trophoblast cells in preeclamptic rats and its potential mechanism.

Methods: Placental trophoblast cells were isolated from preeclamptic model rats and normal ones. Trophoblast cells from the model group were divided into six groups for transfection: the blank (empty vector) group, the NC (negative control) group, the miRNA-335 mimic group, the miRNA-335 inhibitor group, the CRIM1 (overexpressed recombinant plasmid) group, and the miRNA-335 inhibitor + CRIM1 group. The miRNA-335 expressions after the transfection were determined using qRT-PCR. The mRNA and protein expressions of CRIM1, the transforming growth factor (TGF-β1), and the apoptosis-related factors (Bax and Bcl-2) in each group were determined using qRT-PCR and Western blotting. The cell proliferation and apoptosis were determined using MTT assays and flow cytometry, respectively.

Results: Compared with normal rats, the systolic blood pressure, diastolic blood pressure, and urinary protein levels were increased in the model rats, which had increased miRNA-335 expressions, but a decreased CRIM1 expressions (all P<0.05). The inhibition of miRNA-335 promoted the expressions of CRIM1, TGF-β1, and Bcl-2 and inhibited the expression of Bax in trophoblast cells (all P<0.05). miRNA-335 inhibition or CRIM1 over-expression promoted the proliferation and reduced the apoptosis of trophoblast cells. The combined effect of miRNA-335 inhibition or CRIM1 over-expression had an even more significant effect on the changes in the above indicators (all P<0.05).

Conclusion: miRNA-335 inhibition can enhance the expression of CRIM1 to promote the proliferation and reduce the apoptosis of trophoblast cells in preeclamptic rats.

Citing Articles

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References

Herzog E, Eggink A, Reijnierse A, Kerkhof M, de Krijger R, Roks A . Impact of early- and late-onset preeclampsia on features of placental and newborn vascular health. Placenta. 2016; 49:72-79. DOI: 10.1016/j.placenta.2016.11.014. View

Luo R, Shao X, Xu P, Liu Y, Wang Y, Zhao Y . MicroRNA-210 contributes to preeclampsia by downregulating potassium channel modulatory factor 1. Hypertension. 2014; 64(4):839-45. DOI: 10.1161/HYPERTENSIONAHA.114.03530. View

Jiang F, Li J, Wu G, Miao Z, Lu L, Ren G . Upregulation of microRNA‑335 and microRNA‑584 contributes to the pathogenesis of severe preeclampsia through downregulation of endothelial nitric oxide synthase. Mol Med Rep. 2015; 12(4):5383-90. DOI: 10.3892/mmr.2015.4018. View

Ospina-Prieto S, Chaiwangyen W, Herrmann J, Groten T, Schleussner E, Markert U . MicroRNA-141 is upregulated in preeclamptic placentae and regulates trophoblast invasion and intercellular communication. Transl Res. 2016; 172:61-72. DOI: 10.1016/j.trsl.2016.02.012. View

Cushen S, Goulopoulou S . New Models of Pregnancy-Associated Hypertension. Am J Hypertens. 2017; 30(11):1053-1062. DOI: 10.1093/ajh/hpx063. View

Cheng J, Chang H, Fang L, Sun Y, Leung P . TGF-β1 up-regulates connexin43 expression: a potential mechanism for human trophoblast cell differentiation. J Cell Physiol. 2015; 230(7):1558-66. DOI: 10.1002/jcp.24902. View

Li B, Lin L, Yang H, Zhu Y, Wei Y, Li X . The value of the 24-h proteinuria in evaluating the severity of preeclampsia and predicting its adverse maternal outcomes. Hypertens Pregnancy. 2018; 37(3):118-125. DOI: 10.1080/10641955.2018.1487564. View

Jiang J, Zhao Z . LncRNA HOXD-AS1 promotes preeclampsia progression via MAPK pathway. Eur Rev Med Pharmacol Sci. 2018; 22(24):8561-8568. DOI: 10.26355/eurrev_201812_16618. View

Lu T, Lu W, Zhao L . MicroRNA-137 Affects Proliferation and Migration of Placenta Trophoblast Cells in Preeclampsia by Targeting ERRα. Reprod Sci. 2016; 24(1):85-96. DOI: 10.1177/1933719116650754. View

10.

Horvat D, Severson J, Uddin M, Mitchell B, Puschett J . Resibufogenin prevents the manifestations of preeclampsia in an animal model of the syndrome. Hypertens Pregnancy. 2009; 29(1):1-9. DOI: 10.3109/10641950802629709. View

11.

Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H . Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Development. 2013; 141(2):448-59. PMC: 3879820. DOI: 10.1242/dev.097949. View

12.

Song X, Li C, Li J, Liu L, Meng L, Ding H . The long noncoding RNA uc.294 is upregulated in early-onset pre-eclampsia and inhibits proliferation, invasion of trophoblast cells (HTR-8/SVneo). J Cell Physiol. 2018; 234(7):11001-11008. DOI: 10.1002/jcp.27916. View

13.

Bai X, Ma Y, Ding R, Fu B, Shi S, Chen X . miR-335 and miR-34a Promote renal senescence by suppressing mitochondrial antioxidative enzymes. J Am Soc Nephrol. 2011; 22(7):1252-61. PMC: 3137573. DOI: 10.1681/ASN.2010040367. View

14.

Chi Z, Zhang M . Exploration of the regulation and control mechanisms of miR-145 in trophoblast cell proliferation and invasion. Exp Ther Med. 2018; 16(6):5298-5304. PMC: 6257039. DOI: 10.3892/etm.2018.6890. View

15.

Martinez-Fierro M, Castruita-De la Rosa C, Garza-Veloz I, Cardiel-Hernandez R, Espinoza-Juarez M, Delgado-Enciso I . Early pregnancy protein multiplex screening reflects circulating and urinary divergences associated with the development of preeclampsia. Hypertens Pregnancy. 2018; 37(1):37-50. DOI: 10.1080/10641955.2017.1411946. View

16.

Cheng J, Yi Y, Chang H, Leung P . TGF-β1 up-regulates cadherin-11 expression through Snail: A potential mechanism for human trophoblast cell differentiation. Cell Signal. 2017; 43:55-61. DOI: 10.1016/j.cellsig.2017.12.004. View

17.

Yang X, Zhang J, Ding Y . Association of microRNA-155, interleukin 17A, and proteinuria in preeclampsia. Medicine (Baltimore). 2017; 96(18):e6509. PMC: 5419899. DOI: 10.1097/MD.0000000000006509. View

18.

Mason T, Matthews M . Aquatic environment, housing, and management in the eighth edition of the Guide for the Care and Use of Laboratory Animals: additional considerations and recommendations. J Am Assoc Lab Anim Sci. 2012; 51(3):329-32. PMC: 3358981. View

19.

Iyer S, Chhabra Y, Harvey T, Wang R, Chiu H, Smith A . CRIM1 is necessary for coronary vascular endothelial cell development and homeostasis. J Mol Histol. 2016; 48(1):53-61. DOI: 10.1007/s10735-016-9702-3. View

20.

Ding G, Chen M, Wang Y, Rui C, Xu W, Ding H . MicroRNA-128a-induced apoptosis in HTR-8/SVneo trophoblast cells contributes to pre-eclampsia. Biomed Pharmacother. 2016; 81:63-70. DOI: 10.1016/j.biopha.2016.03.040. View