The Effects and Potential Applications of Concentrated Growth Factor in Dentin-pulp Complex Regeneration

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2021 Jun 20

PMID 34147130

Citations 20

Authors

Zixia Li

Liu Liu

Liu Wang

Dongzhe Song

Affiliations

Soon will be listed here.

Abstract

The dentin-pulp complex is essential for the long-term integrity and viability of teeth but it is susceptible to damage caused by external factors. Because traditional approaches for preserving the dentin-pulp complex have various limitations, there is a need for novel methods for dentin-pulp complex reconstruction. The development of stem cell-based tissue engineering has given rise to the possibility of combining dental stem cells with a tissue-reparative microenvironment to promote dentin-pulp complex regeneration. Concentrated growth factor, a platelet concentrate, is a promising scaffold for the treatment of dentin-pulp complex disorders. Given its characteristics of autogenesis, convenience, usability, and biodegradability, concentrated growth factor has gained popularity in medical and dental fields for repairing bone defects and promoting soft-tissue healing. Numerous in vitro studies have demonstrated that concentrated growth factor can promote the proliferation and migration of dental stem cells. Here, we review the current state of knowledge on the effects of concentrated growth factor on stem cells and its potential applications in dentin-pulp complex regeneration.

Citing Articles

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References

Stich S, Loch A, Leinhase I, Neumann K, Kaps C, Sittinger M . Human periosteum-derived progenitor cells express distinct chemokine receptors and migrate upon stimulation with CCL2, CCL25, CXCL8, CXCL12, and CXCL13. Eur J Cell Biol. 2008; 87(6):365-76. DOI: 10.1016/j.ejcb.2008.03.009. View

Son Y, Kang Y, Lee H, Jang S, Bharti D, Lee S . Evaluation of odonto/osteogenic differentiation potential from different regions derived dental tissue stem cells and effect of 17β-estradiol on efficiency. BMC Oral Health. 2021; 21(1):15. PMC: 7792121. DOI: 10.1186/s12903-020-01366-2. View

Takahashi A, Tsujino T, Yamaguchi S, Isobe K, Watanabe T, Kitamura Y . Distribution of platelets, transforming growth factor-β1, platelet-derived growth factor-BB, vascular endothelial growth factor and matrix metalloprotease-9 in advanced platelet-rich fibrin and concentrated growth factor matrices. J Investig Clin Dent. 2019; 10(4):e12458. DOI: 10.1111/jicd.12458. View

Clipet F, Tricot S, Alno N, Massot M, Solhi H, Cathelineau G . In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology. Implant Dent. 2011; 21(1):51-6. DOI: 10.1097/ID.0b013e31822b9cb4. View

Yang J, Yuan G, Chen Z . Pulp Regeneration: Current Approaches and Future Challenges. Front Physiol. 2016; 7:58. PMC: 4779938. DOI: 10.3389/fphys.2016.00058. View

Feng X, Huang D, Lu X, Feng G, Xing J, Lu J . Insulin-like growth factor 1 can promote proliferation and osteogenic differentiation of human dental pulp stem cells via mTOR pathway. Dev Growth Differ. 2014; 56(9):615-24. DOI: 10.1111/dgd.12179. View

Goldberg M, Njeh A, Uzunoglu E . Is Pulp Inflammation a Prerequisite for Pulp Healing and Regeneration?. Mediators Inflamm. 2015; 2015:347649. PMC: 4619968. DOI: 10.1155/2015/347649. View

Takeuchi N, Hayashi Y, Murakami M, Alvarez F, Horibe H, Iohara K . Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte-colony stimulating factor. Oral Dis. 2014; 21(1):113-22. DOI: 10.1111/odi.12227. View

Yu S, Li J, Zhao Y, Li X, Ge L . Comparative Secretome Analysis of Mesenchymal Stem Cells From Dental Apical Papilla and Bone Marrow During Early Odonto/Osteogenic Differentiation: Potential Role of Transforming Growth Factor-β2. Front Physiol. 2020; 11:41. PMC: 7073820. DOI: 10.3389/fphys.2020.00041. View

10.

Tian S, Wang J, Dong F, Du N, Li W, Song P . Concentrated Growth Factor Promotes Dental Pulp Cells Proliferation and Mineralization and Facilitates Recovery of Dental Pulp Tissue. Med Sci Monit. 2019; 25:10016-10028. PMC: 6944166. DOI: 10.12659/MSM.919316. View

11.

Lei G, Yu Y, Jiang Y, Wang S, Yan M, Smith A . Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix. Arch Oral Biol. 2013; 58(7):862-70. DOI: 10.1016/j.archoralbio.2013.01.002. View

12.

Sismanoglu S, Ercal P . Dentin-Pulp Tissue Regeneration Approaches in Dentistry: An Overview and Current Trends. Adv Exp Med Biol. 2020; 1298:79-103. DOI: 10.1007/5584_2020_578. View

13.

Moussa D, Aparicio C . Present and future of tissue engineering scaffolds for dentin-pulp complex regeneration. J Tissue Eng Regen Med. 2018; 13(1):58-75. PMC: 6338516. DOI: 10.1002/term.2769. View

14.

Sun X, Meng L, Qiao W, Yang R, Gao Q, Peng Y . Vascular endothelial growth factor A/Vascular endothelial growth factor receptor 2 axis promotes human dental pulp stem cell migration via the FAK/PI3K/Akt and p38 MAPK signalling pathways. Int Endod J. 2019; 52(12):1691-1703. DOI: 10.1111/iej.13179. View

15.

Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y . Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α. Mol Med Rep. 2018; 19(2):943-950. PMC: 6323209. DOI: 10.3892/mmr.2018.9714. View

16.

Wu M, Chen G, Li Y . TGF-β and BMP signaling in osteoblast, skeletal development, and bone formation, homeostasis and disease. Bone Res. 2016; 4:16009. PMC: 4985055. DOI: 10.1038/boneres.2016.9. View

17.

Rochira A, Siculella L, Damiano F, Palermo A, Ferrante F, Carluccio M . Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells. Biology (Basel). 2020; 9(11). PMC: 7693660. DOI: 10.3390/biology9110370. View

18.

Katagiri T, Watabe T . Bone Morphogenetic Proteins. Cold Spring Harb Perspect Biol. 2016; 8(6). PMC: 4888821. DOI: 10.1101/cshperspect.a021899. View

19.

van Gastel N, Torrekens S, Roberts S, Moermans K, Schrooten J, Carmeliet P . Engineering vascularized bone: osteogenic and proangiogenic potential of murine periosteal cells. Stem Cells. 2012; 30(11):2460-71. DOI: 10.1002/stem.1210. View

20.

Sakai V, Cordeiro M, Dong Z, Zhang Z, Zeitlin B, Nor J . Tooth slice/scaffold model of dental pulp tissue engineering. Adv Dent Res. 2011; 23(3):325-32. PMC: 6699106. DOI: 10.1177/0022034511405325. View