Viability and Infectivity of Gametocytes in Short-Term Culture

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2021 Jun 18

PMID 34141630

Citations 4

Authors

Glenda Quaresma Ramos

Djane Clarys Baia-da-Silva

Marcus Vinicius Guimaraes Lacerda

Wuelton Marcelo Monteiro

Stefanie Costa Pinto Lopes

Affiliations

Soon will be listed here.

Abstract

The control and elimination of malaria caused by both represent a great challenge due to the biological aspects of the species. Gametocytes are the forms responsible for the transmission of the parasite to the vector and the search for new strategies for blocking transmission are essential in a scenario of control and elimination The challenges in this search in regard to mainly stem from the lack of a long-term culture and the limitation of studies of gametocytes. This study evaluated the viability and infectivity of gametocytes in short-term culture. The samples enriched in gametocytes using Percoll (i), using magnetic-activated cell sorting (MACS) (ii), and using non-enriched samples (iii) were evaluated. After the procedures, gametocytes were cultured in IMDM medium for up to 48 h. Cultured gametocytes were viable and infectious for up to 48 h, however differences in viability and infectivity were observed in the samples after 12 h of culture in relation to 0 h. Percoll-enriched samples were shown to be viable in culture for longer intervals than those purified using MACS. Gametocyte viability after enrichment procedures and short-term culture may provide new avenues in the development of methods for evaluating TB.

Citing Articles

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The activity of methylene blue against asexual and sexual stages of .

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