Bioelectrical Methane Production with an Ammonium Oxidative Reaction Under the No Organic Substance Condition

Overview

Journal Microbes Environ

Specialty Environmental Health

Date 2021 Jun 17

PMID 34135211

Citations 2

Authors

Ha T T Dinh

Hiromi Kambara

Yoshiki Harada

Shuji Matsushita

Yoshiteru Aoi

Tomonori Kindaichi

Noriatsu Ozaki

Akiyoshi Ohashi

Affiliations

Soon will be listed here.

Abstract

The present study investigated bioelectrical methane production from CO without organic substances. Even though microbial methane production has been reported at relatively high electric voltages, the amount of voltage required and the organisms contributing to the process currently remain unknown. Methane production using a biocathode was investigated in a microbial electrolysis cell coupled with an NH oxidative reaction at an anode coated with platinum powder under a wide range of applied voltages and anaerobic conditions. A microbial community analysis revealed that methane production simultaneously occurred with biological denitrification at the biocathode. During denitrification, NO was produced by chemical NH oxidation at the anode and was provided to the biocathode chamber. H was produced at the biocathode by the hydrogen-producing bacteria Petrimonas through the acceptance of electrons and protons. The H produced was biologically consumed by hydrogenotrophic methanogens of Methanobacterium and Methanobrevibacter with CO uptake and by hydrogenotrophic denitrifiers of Azonexus. This microbial community suggests that methane is indirectly produced without the use of electrons by methanogens. Furthermore, bioelectrical methane production occurred under experimental conditions even at a very low voltage of 0.05‍ ‍V coupled with NH oxidation, which was thermodynamically feasible.

Citing Articles

Metabolic Potential of the Superphylum Patescibacteria Reconstructed from Activated Sludge Samples from a Municipal Wastewater Treatment Plant.

Fujii N, Kuroda K, Narihiro T, Aoi Y, Ozaki N, Ohashi A Microbes Environ. 2022; 37(3).

PMID: 35768268 PMC: 9530719. DOI: 10.1264/jsme2.ME22012.

Environmental Factors Affecting the Community of Methane-oxidizing Bacteria.

Kambara H, Shinno T, Matsuura N, Matsushita S, Aoi Y, Kindaichi T Microbes Environ. 2022; 37(1).

PMID: 35342121 PMC: 8958294. DOI: 10.1264/jsme2.ME21074.

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