Longitudinal High-resolution Imaging Through a Flexible Intravital Imaging Window

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2021 Jun 17

PMID 34134982

Citations 17

Authors

Guillaume Jacquemin

Maria Benavente-Diaz

Samir Djaber

Aurelien Bore

Virginie Dangles-Marie

Didier Surdez

Shahragim Tajbakhsh

Silvia Fre

Bethan Lloyd-Lewis

Affiliations

Soon will be listed here.

Abstract

Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.

Citing Articles

Methods for Imaging Intracellular Calcium Signals in the Mouse Mammary Epithelium in Two and Three Dimensions.

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Longitudinal intravital microscopy of the mouse kidney: inflammatory responses to abdominal imaging windows.

Martinez M, Walsh J, Kamocka M, Lee H, Dunn K Am J Physiol Renal Physiol. 2024; 327(5):F845-F868.

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In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window.

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Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin.

Takahashi T, Zhang H, Agetsuma M, Nabekura J, Otomo K, Okamura Y Commun Biol. 2024; 7(1):232.

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