ABEL-FRET: Tether-free Single-molecule FRET with Hydrodynamic Profiling

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2021 Jun 15

PMID 34127856

Citations 15

Authors

Hugh Wilson

Quan Wang

Affiliations

Soon will be listed here.

Abstract

Single-molecule Förster resonance energy transfer (smFRET) has become a versatile and widespread method to probe nanoscale conformation and dynamics. However, current experimental modalities often resort to molecule immobilization for long observation times and do not always approach the resolution limit of FRET-based nanoscale metrology. Here we present ABEL-FRET, an immobilization-free platform for smFRET measurements with ultrahigh resolving power in FRET efficiency. Importantly, single-molecule diffusivity is used to provide additional size and shape information for hydrodynamic profiling of individual molecules, which, together with the concurrently measured intramolecular conformation through FRET, enables a holistic and dynamic view of biomolecules and their complexes.

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