A Pan-coronavirus RT-PCR Assay for Rapid Viral Screening of Animal, Human, and Environmental Specimens

Overview

Journal One Health

Specialty Infectious Diseases

Date 2021 Jun 14

PMID 34124332

Citations 1

Authors

Xinye Wang

Leshan Xiu

Raquel A Binder

Teck-Hock Toh

Jeffrey Soon-Yit Lee

Jakie Ting

Son T Than

Wenhao Qi

Kristen K Coleman

David Perera

Maijuan Ma

Gregory C Gray

Affiliations

Soon will be listed here.

Abstract

We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.

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References

Graham B, Corbett K . Prototype pathogen approach for pandemic preparedness: world on fire. J Clin Invest. 2020; 130(7):3348-3349. PMC: 7324162. DOI: 10.1172/JCI139601. View

Toh T, Hii K, Fieldhouse J, Ting J, Berita A, Nguyen T . High Prevalence of Viral Infections Among Hospitalized Pneumonia Patients in Equatorial Sarawak, Malaysia. Open Forum Infect Dis. 2019; 6(3):ofz074. PMC: 6440682. DOI: 10.1093/ofid/ofz074. View

Ma M, Wang G, Anderson B, Bi Z, Lu B, Wang X . Evidence for Cross-species Influenza A Virus Transmission Within Swine Farms, China: A One Health, Prospective Cohort Study. Clin Infect Dis. 2018; 66(4):533-540. PMC: 5848305. DOI: 10.1093/cid/cix823. View

Geoghegan J, Holmes E . Predicting virus emergence amid evolutionary noise. Open Biol. 2017; 7(10). PMC: 5666085. DOI: 10.1098/rsob.170189. View

Le Y, Nguyen K, Coleman K, Nguyen T, Than S, Phan H . Virus detections among patients with severe acute respiratory illness, Northern Vietnam. PLoS One. 2020; 15(5):e0233117. PMC: 7217455. DOI: 10.1371/journal.pone.0233117. View

Pepin K, Miller R, Wilber M . A framework for surveillance of emerging pathogens at the human-animal interface: Pigs and coronaviruses as a case study. Prev Vet Med. 2021; 188:105281. PMC: 7839430. DOI: 10.1016/j.prevetmed.2021.105281. View

Borkenhagen L, Mallinson K, Tsao R, Ha S, Lim W, Toh T . Surveillance for respiratory and diarrheal pathogens at the human-pig interface in Sarawak, Malaysia. PLoS One. 2018; 13(7):e0201295. PMC: 6063427. DOI: 10.1371/journal.pone.0201295. View

Vlasova A, Diaz A, Damtie D, Xiu L, Toh T, Lee J . Novel Canine Coronavirus Isolated from a Hospitalized Patient With Pneumonia in East Malaysia. Clin Infect Dis. 2021; 74(3):446-454. PMC: 8194511. DOI: 10.1093/cid/ciab456. View

Xiu L, Binder R, Alarja N, Kochek K, Coleman K, Than S . A RT-PCR assay for the detection of coronaviruses from four genera. J Clin Virol. 2020; 128:104391. PMC: 7192118. DOI: 10.1016/j.jcv.2020.104391. View

10.

Crawford E, Kamm J, Miller S, Li L, Caldera S, Lyden A . Investigating Transfusion-related Sepsis Using Culture-Independent Metagenomic Sequencing. Clin Infect Dis. 2019; 71(5):1179-1185. PMC: 7442849. DOI: 10.1093/cid/ciz960. View

11.

Fieldhouse J, Bailey E, Toh T, Hii K, Mallinson K, Ting J . Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia. Trop Dis Travel Med Vaccines. 2020; 6:13. PMC: 7422451. DOI: 10.1186/s40794-020-00114-2. View

12.

Carlson C . From PREDICT to prevention, one pandemic later. Lancet Microbe. 2020; 1(1):e6-e7. PMC: 7148715. DOI: 10.1016/S2666-5247(20)30002-1. View

13.

Gray G, Robie E, Studstill C, Nunn C . Mitigating Future Respiratory Virus Pandemics: New Threats and Approaches to Consider. Viruses. 2021; 13(4). PMC: 8068197. DOI: 10.3390/v13040637. View

14.

Pickering B, Smith G, Pinette M, Embury-Hyatt C, Moffat E, Marszal P . Susceptibility of Domestic Swine to Experimental Infection with Severe Acute Respiratory Syndrome Coronavirus 2. Emerg Infect Dis. 2020; 27(1):104-112. PMC: 7774549. DOI: 10.3201/eid2701.203399. View

15.

Holmes E, Rambaut A, Andersen K . Pandemics: spend on surveillance, not prediction. Nature. 2018; 558(7709):180-182. DOI: 10.1038/d41586-018-05373-w. View

16.

Edwards C, Yount B, Graham R, Leist S, Hou Y, Dinnon 3rd K . Swine acute diarrhea syndrome coronavirus replication in primary human cells reveals potential susceptibility to infection. Proc Natl Acad Sci U S A. 2020; 117(43):26915-26925. PMC: 7604506. DOI: 10.1073/pnas.2001046117. View

17.

Li W, Hulswit R, Kenney S, Widjaja I, Jung K, Alhamo M . Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibility. Proc Natl Acad Sci U S A. 2018; 115(22):E5135-E5143. PMC: 5984533. DOI: 10.1073/pnas.1802879115. View

18.

Gray G, Abdelgadir A . While We Endure This Pandemic, What New Respiratory Virus Threats Are We Missing?. Open Forum Infect Dis. 2021; 8(3):ofab078. PMC: 7928563. DOI: 10.1093/ofid/ofab078. View

19.

Wang X, Bailey E, Qi X, Yu H, Bao C, Gray G . Bioaerosol Sampling at a Live Animal Market in Kunshan, China: A Noninvasive Approach for Detecting Emergent Viruses. Open Forum Infect Dis. 2020; 7(5):ofaa134. PMC: 7240344. DOI: 10.1093/ofid/ofaa134. View