A Method to Quantify Behavioral Activities Induced by GABA Agonist Muscimol

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Jun 14

PMID 34124286

Citations 1

Authors

M Rezaul Karim

Affiliations

Soon will be listed here.

Abstract

Muscimol is a psychoactive isoxazole derived from the mushroom . As a potent GABA receptor agonist, muscimol suppresses the activity of the central nervous system, reduces anxiety and induces sleep. We investigated the effects of muscimol on behavior. behavioral assays are powerful tools that are used to assess neural functions by focusing on specific changes in selected behavior, with the hypothesis that this behavioral change is due to alteration of the underlying neural function of interest. In this study, we developed a comparatively simple and cost-effective method for feeding adult flies muscimol, a pharmacologically active compound, and for quantifying the phenotypes of "resting" and "grooming+walking". This protocol may provide researchers with a convenient method to characterize small molecule-induced behavioral output in flies.

Citing Articles

Intricate mechanism of anxiety disorder, recognizing the potential role of gut microbiota and therapeutic interventions.

Lakhawat S, Mech P, Kumar A, Malik N, Kumar V, Sharma V Metab Brain Dis. 2024; 40(1):64.

PMID: 39671133 DOI: 10.1007/s11011-024-01453-1.

References

Pitmon E, Stephens G, Parkhurst S, Wolf F, Kehne G, Taylor M . The D1 family dopamine receptor, DopR, potentiates hind leg grooming behavior in Drosophila. Genes Brain Behav. 2016; 15(3):327-34. PMC: 5021212. DOI: 10.1111/gbb.12264. View

Hampel S, Franconville R, Simpson J, Seeds A . A neural command circuit for grooming movement control. Elife. 2015; 4:e08758. PMC: 4599031. DOI: 10.7554/eLife.08758. View

Mackay T, Anholt R . Of flies and man: Drosophila as a model for human complex traits. Annu Rev Genomics Hum Genet. 2006; 7:339-67. DOI: 10.1146/annurev.genom.7.080505.115758. View

Kaur K, Simon A, Chauhan V, Chauhan A . Effect of bisphenol A on Drosophila melanogaster behavior--a new model for the studies on neurodevelopmental disorders. Behav Brain Res. 2015; 284:77-84. DOI: 10.1016/j.bbr.2015.02.001. View

Zhukovskaya M, Yanagawa A, Forschler B . Grooming Behavior as a Mechanism of Insect Disease Defense. Insects. 2015; 4(4):609-30. PMC: 4553506. DOI: 10.3390/insects4040609. View

Yoshimoto M, Higuchi H, Kamata M, Yoshida K, Shimizu T, Hishikawa Y . The effects of benzodiazepine (triazolam), cyclopyrrolone (zopiclone) and imidazopyridine (zolpidem) hypnotics on the frequency of hippocampal theta activity and sleep structure in rats. Eur Neuropsychopharmacol. 1999; 9(1-2):29-35. DOI: 10.1016/s0924-977x(97)00102-8. View

Chen A, Wilburn P, Hao X, Tully T . Walking deficits and centrophobism in an α-synuclein fly model of Parkinson's disease. Genes Brain Behav. 2014; 13(8):812-20. PMC: 4262005. DOI: 10.1111/gbb.12172. View

Hidalgo S, Molina-Mateo D, Escobedo P, Zarate R, Fritz E, Fierro A . Characterization of a Novel Drosophila SERT Mutant: Insights on the Contribution of the Serotonin Neural System to Behaviors. ACS Chem Neurosci. 2017; 8(10):2168-2179. DOI: 10.1021/acschemneuro.7b00089. View

Zhang X, Yao N, Chergui K . The GABAA receptor agonist muscimol induces an age- and region-dependent form of long-term depression in the mouse striatum. Learn Mem. 2016; 23(9):479-85. PMC: 4986858. DOI: 10.1101/lm.043190.116. View

10.

Karim M, Yanagawa A, Ohinata K . Soy undecapeptide induces Drosophila hind leg grooming via dopamine receptor. Biochem Biophys Res Commun. 2018; 499(3):454-458. DOI: 10.1016/j.bbrc.2018.03.162. View

11.

Lancel M . Role of GABAA receptors in the regulation of sleep: initial sleep responses to peripherally administered modulators and agonists. Sleep. 1999; 22(1):33-42. DOI: 10.1093/sleep/22.1.33. View

12.

Biggio G, Casu M, Corda M, Vernaleone F, Gessa G . Effect of muscimol, a GABA-mimetic agent, on dopamine metabolism in the mouse brain. Life Sci. 1977; 21(4):525-31. DOI: 10.1016/0024-3205(77)90092-3. View

13.

Kumar A, Kalonia H . Effect of Withania somnifera on Sleep-Wake Cycle in Sleep-Disturbed Rats: Possible GABAergic Mechanism. Indian J Pharm Sci. 2011; 70(6):806-10. PMC: 3040882. DOI: 10.4103/0250-474X.49130. View

14.

Rissman R, Mobley W . Implications for treatment: GABAA receptors in aging, Down syndrome and Alzheimer's disease. J Neurochem. 2011; 117(4):613-22. PMC: 3127285. DOI: 10.1111/j.1471-4159.2011.07237.x. View