Assessment of Significant Pathway Signaling and Prognostic Value of GNG11 in Ovarian Serous Cystadenocarcinoma

Overview

Journal Int J Gen Med

Publisher Dove Medical Press

Specialty General Medicine

Date 2021 Jun 11

PMID 34113163

Citations 7

Authors

Ming-Min Jiang

Fan Zhao

Tao-Tao Lou

Affiliations

Soon will be listed here.

Abstract

Background: GNG11 (G protein subunit gamma 11) is a member of guanine nucleotide-binding protein (G protein) gamma family. Few studies elucidated the role of GNG11 in human disease, especially in tumors. The present study initially analyzed the function of GNG11 in ovarian serous cystadenocarcinoma.

Methods: The differential expression of GNG11 mRNA in ovarian cancer and normal tissues was evaluated through Oncomine, CCLE, Gepia, UCSC Xena and UALCAN databases. The protein expression of GNG11 was assessed via HPA database. Prognosis analysis was performed by Kaplan-Meier Plotter. Restrict survival analysis to subtypes including tumor grade, cancer stage and TP53 mutation status was then carried out. GSEA enrichment analysis was performed to explore the significant pathways associated with GNG11 in ovarian cancer. Finally, the upstream miRNAs of GNG11 were predicted by DIANA, Target Scan, miRDB and miRWalk databases, and the potential key KEGG pathways were subsequently determined by DIANA.

Results: The mRNA expression of GNG11 was down-regulated in ovarian cancer patients (<0.05). The cancer stage of patients correlated with the expression of GNG11 (<0.05). Survival analysis indicated that GNG11 high expression statistically shortened the overall survival time of patients (HR=1.26, =0.0043) compared with low expression group, especially for the patients with earlier stage (HR=2.48, =0.035) and lower grade (HR=1.72, =0.0016). Subsequently, the consistent upstream miRNA of GNG11, hsa-miR-22-5p, was predicted from 4 databases. The differential expression profile of hsa-miR-22-5p in blood was observed in ovarian cancer patients. According to the GSEA analysis on GNG11 and KEGG analysis on hsa-miR-22-5p, the consistent pathway of ECM-receptor interaction was observed (all <0.01). ECM-receptor interaction pathway and differential expression of hsa-miR-22-5p in blood suggested the migration risk of ovarian cancer.

Conclusion: High expression of GNG11 indicated the poor prognosis of ovarian cancer patients. GNG11 might play a crucial role in the biological process of ovarian serous cystadenocarcinoma by ECM-receptor interaction pathway, thus affecting the prognosis of patients.

Citing Articles

Identification of key regulators in pancreatic ductal adenocarcinoma using network theoretical approach.

Bhattacharjee K, Ghosh A PLoS One. 2025; 20(1):e0313738.

PMID: 39869563 PMC: 11771905. DOI: 10.1371/journal.pone.0313738.

Partial Inhibition of Epithelial-to-Mesenchymal Transition (EMT) Phenotypes by Placenta-Derived DBMSCs in Human Breast Cancer Cell Lines, In Vitro.

Basmaeil Y, Subayyil A, Kulayb H, Kondkar A, Alrodayyan M, Khatlani T Cells. 2025; 13(24.

PMID: 39768220 PMC: 11674051. DOI: 10.3390/cells13242131.

A novel platelets-related gene signature for predicting prognosis, immune features and drug sensitivity in gastric cancer.

Li Q, Zhang C, Ren Y, Qiao L, Xu S, Li K Front Immunol. 2024; 15:1477427.

PMID: 39606245 PMC: 11599260. DOI: 10.3389/fimmu.2024.1477427.

Overall survival prediction of gastric cancer using the gene signature of CT-detected extramural venous invasion combined with M2 macrophages infiltration.

Yang H, Gou X, Feng C, Zhang Y, Sun B, Peng P J Transl Med. 2024; 22(1):829.

PMID: 39252063 PMC: 11382430. DOI: 10.1186/s12967-024-05628-3.

Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells.

Meng G, Duan H, Jia J, Liu B, Ma Y, Cai X Anim Biosci. 2024; 37(3):509-521.

PMID: 38271979 PMC: 10915198. DOI: 10.5713/ab.23.0370.

References

Shi K, Li N, Yang M, Li W . Identification of Key Genes and Pathways in Female Lung Cancer Patients Who Never Smoked by a Bioinformatics Analysis. J Cancer. 2019; 10(1):51-60. PMC: 6329865. DOI: 10.7150/jca.26908. View

Peng W, Li J, Zeng J, Zou X, Tang D, Tang W . Clinical value and potential mechanisms of COL8A1 upregulation in breast cancer: a comprehensive analysis. Cancer Cell Int. 2020; 20:392. PMC: 7427770. DOI: 10.1186/s12935-020-01465-8. View

Kaern J, Aghmesheh M, Nesland J, Danielsen H, Sandstad B, Friedlander M . Prognostic factors in ovarian carcinoma stage III patients. Can biomarkers improve the prediction of short- and long-term survivors?. Int J Gynecol Cancer. 2005; 15(6):1014-22. DOI: 10.1111/j.1525-1438.2005.00185.x. View

Vang R, Levine D, Soslow R, Zaloudek C, Shih I, Kurman R . Molecular Alterations of TP53 are a Defining Feature of Ovarian High-Grade Serous Carcinoma: A Rereview of Cases Lacking TP53 Mutations in The Cancer Genome Atlas Ovarian Study. Int J Gynecol Pathol. 2015; 35(1):48-55. PMC: 4696053. DOI: 10.1097/PGP.0000000000000207. View

Hua P, Zhang Y, Jin C, Zhang G, Wang B . Integration of gene profile to explore the hub genes of lung adenocarcinoma: A quasi-experimental study. Medicine (Baltimore). 2020; 99(43):e22727. PMC: 7581154. DOI: 10.1097/MD.0000000000022727. View

Torre L, Islami F, Siegel R, Ward E, Jemal A . Global Cancer in Women: Burden and Trends. Cancer Epidemiol Biomarkers Prev. 2017; 26(4):444-457. DOI: 10.1158/1055-9965.EPI-16-0858. View

Bachmayr-Heyda A, Aust S, Auer K, Meier S, Schmetterer K, Dekan S . Integrative Systemic and Local Metabolomics with Impact on Survival in High-Grade Serous Ovarian Cancer. Clin Cancer Res. 2016; 23(8):2081-2092. DOI: 10.1158/1078-0432.CCR-16-1647. View

Allemani C, Weir H, Carreira H, Harewood R, Spika D, Wang X . Global surveillance of cancer survival 1995-2009: analysis of individual data for 25,676,887 patients from 279 population-based registries in 67 countries (CONCORD-2). Lancet. 2014; 385(9972):977-1010. PMC: 4588097. DOI: 10.1016/S0140-6736(14)62038-9. View

Layeghi S, Arabpour M, Esmaeili R, Naghizadeh M, Tavakkoly Bazzaz J, Shakoori A . Evaluation of the potential role of long non-coding RNA LINC00961 in luminal breast cancer: a case-control and systems biology study. Cancer Cell Int. 2020; 20:478. PMC: 7531117. DOI: 10.1186/s12935-020-01569-1. View

10.

Xing S, Wang Y, Hu K, Wang F, Sun T, Li Q . WGCNA reveals key gene modules regulated by the combined treatment of colon cancer with PHY906 and CPT11. Biosci Rep. 2020; 40(9). PMC: 7468096. DOI: 10.1042/BSR20200935. View

11.

Liu J, Li S, Lin L, Jiang Y, Wan Y, Zhou S . Co-expression network analysis identified atypical chemokine receptor 1 (ACKR1) association with lymph node metastasis and prognosis in cervical cancer. Cancer Biomark. 2020; 27(2):213-223. DOI: 10.3233/CBM-190533. View

12.

Zheng M, Li X, Hu Y, Dong H, Gou R, Nie X . Identification of molecular marker associated with ovarian cancer prognosis using bioinformatics analysis and experiments. J Cell Physiol. 2019; 234(7):11023-11036. DOI: 10.1002/jcp.27926. View

13.

Wu M, Sun Y, Wu J, Liu G . Identification of Hub Genes in High-Grade Serous Ovarian Cancer Using Weighted Gene Co-Expression Network Analysis. Med Sci Monit. 2020; 26:e922107. PMC: 7101203. DOI: 10.12659/MSM.922107. View

14.

Slomovitz B, Gourley C, Carey M, Malpica A, Shih I, Huntsman D . Low-grade serous ovarian cancer: State of the science. Gynecol Oncol. 2020; 156(3):715-725. DOI: 10.1016/j.ygyno.2019.12.033. View

15.

Shapira I, Oswald M, Lovecchio J, Khalili H, Menzin A, Whyte J . Circulating biomarkers for detection of ovarian cancer and predicting cancer outcomes. Br J Cancer. 2013; 110(4):976-83. PMC: 3929876. DOI: 10.1038/bjc.2013.795. View

16.

Siegel R, Miller K, Jemal A . Cancer statistics, 2019. CA Cancer J Clin. 2019; 69(1):7-34. DOI: 10.3322/caac.21551. View

17.

Zhang H, Tao J, Sheng L, Hu X, Rong R, Xu M . Twist2 promotes kidney cancer cell proliferation and invasion by regulating ITGA6 and CD44 expression in the ECM-receptor interaction pathway. Onco Targets Ther. 2016; 9:1801-12. PMC: 4821395. DOI: 10.2147/OTT.S96535. View

18.

Hossain M, Sakemura R, Fujii M, Ayusawa D . G-protein gamma subunit GNG11 strongly regulates cellular senescence. Biochem Biophys Res Commun. 2006; 351(3):645-50. DOI: 10.1016/j.bbrc.2006.10.112. View

19.

Yuan J, Xie A, Cao Q, Li X, Chen J . INHBB Is a Novel Prognostic Biomarker Associated with Cancer-Promoting Pathways in Colorectal Cancer. Biomed Res Int. 2020; 2020:6909672. PMC: 7563060. DOI: 10.1155/2020/6909672. View

20.

Fransson A, Glaessgen D, Alfredsson J, Wiman K, Bajalica-Lagercrantz S, Mohell N . Strong synergy with APR-246 and DNA-damaging drugs in primary cancer cells from patients with TP53 mutant High-Grade Serous ovarian cancer. J Ovarian Res. 2016; 9(1):27. PMC: 4868029. DOI: 10.1186/s13048-016-0239-6. View