Next‑generation Sequencing of MiRNAs and LncRNAs from Rat Femur and Tibia Under Mechanical Stress

Overview

Journal Mol Med Rep

Specialty Molecular Biology

Date 2021 Jun 10

PMID 34109424

Citations 2

Authors

Yiyan Qiu

Guozheng Zhu

Canjun Zeng

Song Yuan

Yuepeng Qian

Zelin Ye

Shanwen Zhao

Runguang Li

Affiliations

Soon will be listed here.

Abstract

Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated the exercise‑induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation in femur and tibia by establishing an animal exercise model using a treadmill exercise system. MicroRNA (miRNA/miR) and long non‑coding (lnc)RNA sequencing analyses identified 16 upregulated and two downregulated miRNAs in the exercise group, as well as 44 upregulated lncRNAs and 39 downregulated lncRNAs in the exercise group. There was increased expression of miR‑9942 and miR‑7704 in both the femur and tibia and an upregulation of miR‑30d, miR‑5100 and miR‑1260 in the femur of animals from the exercise group. In addition, four of the five most downregulated lncRNAs, including lncRNA MSTRG.2625, lncRNA MSTRG.1557, lncRNA MSTRG.691 and lncRNA MSTRG.7497, were demonstrated to be suppressed in both the femur and tibia after treadmill exercise. The results of the present study provided a valuable resource for further exploring the molecular mechanisms underlying the regulation of osteoporosis by exercise.

Citing Articles

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Whole Transcriptome Mapping Identifies an Immune- and Metabolism-Related Non-coding RNA Landscape Remodeled by Mechanical Stress in IL-1β-Induced Rat OA-like Chondrocytes.

Zhang J, Hao X, Chi R, Liu J, Shang X, Deng X Front Genet. 2022; 13:821508.

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