Culture Conditions for Arresting and Stimulating the Proliferation of a Rainbow Trout Fibroblast Cell Line, RTG-2

Overview

Journal In Vitro Cell Dev Biol

Specialties Anatomy & Histology
Cell Biology

Date 1988 Aug 1

PMID 3410806

Citations 5

Authors

L E Lee

A Martinez

N C Bols

Affiliations

Soon will be listed here.

Abstract

Conditions for arresting and stimulating the proliferation of the rainbow trout fibroblast cell line RTG-2 have been examined and the time course of events after stimulation determined. Quiescent populations were achieved in two ways. Cultures grown to confluency without a medium change for at least 7 d had fewer than 5% of the cells in S phase and few mitotic figures. Cultures deprived of serum, which could be done for up to 3 d without a loss in cell number, also achieved quiescence. After 3 d without serum, less than 1% of cells were in S phase and mitotic figures were infrequent. Addition to these cultures of fresh serum-containing medium brought about the synchronous entry of cells into S phase and mitosis. For cultures in which either the medium had been changed after 7 d without a change or serum-containing medium had been added after 3 d of serum deprivation, DNA synthesis increased after a lag period of 20 to 24 h, was pronounced between 30 and 45 h, and then declined. This was followed by a peak in the mitotic index. These protocols for arresting and subsequently stimulating RTG-2 proliferation should allow the G1-S transition to be studied in a representative of teleosts.

Citing Articles

Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.

Bols N, Pham P, Dayeh V, Lee L In Vitro Cell Dev Biol Anim. 2017; 53(5):383-405.

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Identification of a heparin-binding, mesoderm-inducing peptide in the swim-bladder of the red seabream, Pagrus major: a probable fish fibroblast growth factor.

Suzuki T, Kurokawa T, Asashima M Fish Physiol Biochem. 2013; 13(4):343-52.

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Technology and uses of cell cultures from the tissues and organs of bony fish.

Bols N, Lee L Cytotechnology. 2012; 6(3):163-87.

PMID: 22359161 DOI: 10.1007/BF00624756.

Characterization of primary culture of rainbow trout (Oncorhynchus mykiss) skin explants: growth, cell composition, proliferation, and apoptosis.

Nolan D, Nabben I, Li J, Wendelaar Bonga S In Vitro Cell Dev Biol Anim. 2002; 38(1):14-24.

PMID: 11963963 DOI: 10.1290/1071-2690(2002)038<0014:COPCOR>2.0.CO;2.

Mitogenic activity from trout embryos.

Collodi P, Barnes D Proc Natl Acad Sci U S A. 1990; 87(9):3498-502.

PMID: 2333296 PMC: 53928. DOI: 10.1073/pnas.87.9.3498.

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