Substance P: Characteristics of Binding to Synaptic Vesicles of Rat Brain

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1979 Jan 31

PMID 34108

Citations 3

Authors

N Mayer

F Lembeck

A Saria

R Gamse

Affiliations

Soon will be listed here.

Abstract

1. The binding of substance P (SP) to synaptic vesicles from rat brain was studied by use of the 125I-Tyr8-analogue of SP. 2. The pH dependence of the binding of both peptides to the lipid extractable fraction of synaptic vesicles was shown to be comparable. 3. The binding of 125I-Tyr8-SP shows a rate constant of association (k1 = 6.6 x 10(6) M-1 S-1), a rate constant of dissociation (k-1 = 6.4 x 10(-4) S-1) and gives a KD of 1 x 10(-10) M. Kd derived from equilibrium studies was 3.2 x 10(-10) M. 4. The binding of 125I-Tyr8-SP to lipids of synaptic vesicles was shown to be reversible, saturable and highly specific. 5. The kinetic data suggest one population of binding sites with a maximal number of 0.8 pmol per mg protein of the synaptic vesicle preparation. 6. Unlabeled SP and the (2--11)-, (3--11)- and (4--11)-analogues of SP inhibit the binding of 125I-Tyr8-SP in a decreasing order in a competitive way when added in excess. Tyr8-SP and eledoisin did not interfere with the binding of 125I-Tyr8-SP whereas uperolein and neurotensin caused a partial inhibition. Physalaemin and D-Ala2-D-Met5-enkephalin enhance the binding of 125I-Tyr8-SP in a cooperative way.

Citing Articles

Properties of a 125I-substance P derivative binding to synaptosomes from various brain structures and the spinal cord of the rat.

Torrens Y, Beaujouan J, Viger A, Glowinski J Naunyn Schmiedebergs Arch Pharmacol. 1983; 324(2):134-9.

PMID: 6196645 DOI: 10.1007/BF00497019.

Regional distribution and biochemical properties of 125I-Tyr8-substance P binding sites in synaptic vesicles.

Saria A, Mayer N, Lembeck F, Pabst M Naunyn Schmiedebergs Arch Pharmacol. 1980; 311(2):151-7.

PMID: 6155617 DOI: 10.1007/BF00510254.

Substance P in the vagus nerve. Immunochemical and immunohistochemical evidence for axoplasmic transport.

Gamse R, Lembeck F, Cuello A Naunyn Schmiedebergs Arch Pharmacol. 1979; 306(1):37-44.

PMID: 85263 DOI: 10.1007/BF00515591.

References

Otsuka M, Konishi S . Release of substance P-like immunoreactivity from isolated spinal cord of newborn rat. Nature. 1976; 264(5581):83-4. DOI: 10.1038/264083a0. View

Lembeck F, Mayer N, Schindler G . Substance P: binding to lipids in the brain. Naunyn Schmiedebergs Arch Pharmacol. 1978; 303(1):79-86. DOI: 10.1007/BF00496188. View

Pickel V, Reis D, Leeman S . Ultrastructural localization of substance P in neurons of rat spinal cord. Brain Res. 1977; 122(3):534-40. DOI: 10.1016/0006-8993(77)90463-2. View

Glossmann H, Baukal A, Catt K . Properties of angiotensin II receptors in the bovine and rat adrenal cortex. J Biol Chem. 1974; 249(3):825-34. View

Davis M, Akera T, BRODY T, Watson L . Opiate receptor: cooperativity of binding observed in brain slices. Proc Natl Acad Sci U S A. 1977; 74(12):5764-6. PMC: 431874. DOI: 10.1073/pnas.74.12.5764. View

Loh H, Cho T, Wu Y, WAY E . Stereospecific binding of narcotics to brain cerebrosides. Life Sci. 1974; 14(11):2231-45. DOI: 10.1016/0024-3205(74)90105-2. View

Johnson D, Cho T, Loh H . The binding of 5-hydroxytryptamine to acidic lipids in isobutanol. J Neurochem. 1977; 29(6):1101-3. DOI: 10.1111/j.1471-4159.1977.tb06514.x. View

Chang M, Leeman S . Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterization as substance P. J Biol Chem. 1970; 245(18):4784-90. View

Nakata Y, Kusaka Y, Segawa T, Yajima H, Kitagawa K . Substance P: regional distribution and specific binding to synaptic membranes in rabbit central nervous system. Life Sci. 1978; 22(3):259-67. DOI: 10.1016/0024-3205(78)90527-1. View

10.

Devynck M, Meyer P . Angiotensin receptors. Biochem Pharmacol. 1978; 27(1):1-5. DOI: 10.1016/0006-2952(78)90249-6. View

11.

Kitabgi P, Carraway R, Van Rietschoten J, Granier C, Morgat J, Menez A . Neurotensin: specific binding to synaptic membranes from rat brain. Proc Natl Acad Sci U S A. 1977; 74(5):1846-50. PMC: 431025. DOI: 10.1073/pnas.74.5.1846. View

12.

Lembeck F, Mayer N, Schindler G . Substance P in rat brain synaptosomes. Naunyn Schmiedebergs Arch Pharmacol. 1977; 301(1):17-22. DOI: 10.1007/BF00501259. View

13.

Miller R, Chang K, LEIGHTON J, Cuatrecasas P . Interaction of iodinated enkephalin analogues with opiate receptors. Life Sci. 1978; 22(5):379-88. DOI: 10.1016/0024-3205(78)90284-9. View

14.

Schenker C, Mroz E, Leeman S . Release of substance P from isolated nerve endings. Nature. 1976; 264(5588):790-2. DOI: 10.1038/264790a0. View

15.

Simantov R, Childers S, Snyder S . The opiate receptor binding interactions of 3H-methionine enkephalin, an opioid peptide. Eur J Pharmacol. 1978; 47(3):319-31. DOI: 10.1016/0014-2999(78)90240-6. View

16.

Iversen L, Jessell T, Kanazawa I . Release and metabolism of substance P in rat hypothalamus. Nature. 1976; 264(5581):81-3. DOI: 10.1038/264081a0. View

17.

Ryall R . THE SUBCELLULAR DISTRIBUTIONS OF ACETYLCHOLINE, SUBSTANCE P, 5-HYDROXYTRYPTAMINE, GAMMA-AMINOBUTYRIC ACID AND GLUTAMIC ACID IN BRAIN HOMOGENATES. J Neurochem. 1964; 11:131-45. DOI: 10.1111/j.1471-4159.1964.tb06124.x. View

18.

Heizmann A, Lembeck F, Seidel G . [Obtaining substance P from the lipid extract of brain tissue]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1966; 253(2):265-79. View

19.

Cuello A, Jessell T, Kanazawa I, Iversen L . Substance P: localization in synaptic vesicles in rat central nervous system. J Neurochem. 1977; 29(4):747-51. DOI: 10.1111/j.1471-4159.1977.tb07795.x. View

20.

Uhl G, Snyder S . Neurotensin receptor binding, regional and subcellular distributions favor transmitter role. Eur J Pharmacol. 1977; 41(1):89-91. DOI: 10.1016/0014-2999(77)90378-8. View