Cannabinoid Receptors Distribution in Mouse Cortical Plasma Membrane Compartments

Overview

Journal Mol Brain

Publisher Biomed Central

Specialties Molecular Biology
Neurology

Date 2021 Jun 8

PMID 34099009

Citations 8

Authors

Hajar Miranzadeh Mahabadi

Haseeb Bhatti

Robert B Laprairie

Changiz Taghibiglou

Affiliations

Soon will be listed here.

Abstract

The type 1 and type 2 cannabinoid receptors (CB1 and CB2 receptors) are class A G protein-coupled receptors (GPCRs) that are activated by endogenous lipids called endocannabinoids to modulate neuronal excitability and synaptic transmission in neurons throughout the central nervous system (CNS), and inflammatory processes throughout the body. CB1 receptor is one of the most abundant GPCRs in the CNS and is involved in many physiological and pathophysiological processes, including mood, appetite, and nociception. CB2 receptor is primarily found on immunomodulatory cells of both the CNS and the peripheral immune system. In this study, we isolated lipid raft and non-lipid raft fractions of plasma membrane (PM) from mouse cortical tissue by using cold non-ionic detergent and sucrose gradient centrifugation to study the localization of CB1 receptor and CB2 receptor. Lipid raft and non-lipid raft fractions were confirmed by flotillin-1, caveolin-1 and transferrin receptor as their protein biomarkers. Both CB1 receptor and CB2 receptor were found in non-raft compartments that is inconsistent with previous findings in cultured cell lines. This study demonstrates compartmentalization of both CB1 receptor and CB2 receptor in cortical tissue and warrants further investigation of CB1 receptor and CB2 receptor compartmental distribution in various brain regions and cell types.

Citing Articles

JWH-182: a safe and effective synthetic cannabinoid for chemotherapy-induced neuropathic pain in preclinical models.

Filipiuc L, Creanga-Murariu I, Tamba B, Ababei D, Rusu R, Stanciu G Sci Rep. 2024; 14(1):16242.

PMID: 39004628 PMC: 11247095. DOI: 10.1038/s41598-024-67154-y.

Milk-based culture of and its component oleamide affect cognitive function in healthy elderly Japanese individuals: a multi-arm randomized, double-blind, placebo-controlled study.

Sasaki M, Oba C, Nakamura K, Takeo H, Toya H, Furuichi K Front Nutr. 2024; 11:1357920.

PMID: 38600994 PMC: 11004446. DOI: 10.3389/fnut.2024.1357920.

The Healthy and Diseased Retina Seen through Neuron-Glia Interactions.

Tempone M, Borges-Martins V, Cesar F, Alexandrino-Mattos D, de Figueiredo C, Raony I Int J Mol Sci. 2024; 25(2).

PMID: 38256192 PMC: 10817105. DOI: 10.3390/ijms25021120.

Update on the controversial identity of cells expressing cnr2 gene in the nervous system.

Grabon W, Bodennec J, Rheims S, Belmeguenai A, Bezin L CNS Neurosci Ther. 2023; 29(3):760-770.

PMID: 36604187 PMC: 9928557. DOI: 10.1111/cns.13977.

Cannabinoid type-2 receptors: An emerging target for regulating schizophrenia-relevant brain circuits.

Ferranti A, Foster D Front Neurosci. 2022; 16:925792.

PMID: 36033626 PMC: 9403189. DOI: 10.3389/fnins.2022.925792.

References

TSOU K, Brown S, Mackie K, Walker J . Immunohistochemical distribution of cannabinoid CB1 receptors in the rat central nervous system. Neuroscience. 1998; 83(2):393-411. DOI: 10.1016/s0306-4522(97)00436-3. View

Brailoiu G, Oprea T, Zhao P, Abood M, Brailoiu E . Intracellular cannabinoid type 1 (CB1) receptors are activated by anandamide. J Biol Chem. 2011; 286(33):29166-29174. PMC: 3190724. DOI: 10.1074/jbc.M110.217463. View

Peter A, Weigert C, Staiger H, Machicao F, Schick F, Machann J . Individual stearoyl-coa desaturase 1 expression modulates endoplasmic reticulum stress and inflammation in human myotubes and is associated with skeletal muscle lipid storage and insulin sensitivity in vivo. Diabetes. 2009; 58(8):1757-65. PMC: 2712792. DOI: 10.2337/db09-0188. View

Howlett A, Abood M . CB and CB Receptor Pharmacology. Adv Pharmacol. 2017; 80:169-206. PMC: 5812699. DOI: 10.1016/bs.apha.2017.03.007. View

Zhang K, Yang Q, Yang L, Li Y, Wang X, Li Y . CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest. 2019; 129(6):2333-2350. PMC: 6546454. DOI: 10.1172/JCI123689. View

Levental I, Veatch S . The Continuing Mystery of Lipid Rafts. J Mol Biol. 2016; 428(24 Pt A):4749-4764. PMC: 5124408. DOI: 10.1016/j.jmb.2016.08.022. View

Rimmerman N, Bradshaw H, Kozela E, Levy R, Juknat A, Vogel Z . Compartmentalization of endocannabinoids into lipid rafts in a microglial cell line devoid of caveolin-1. Br J Pharmacol. 2011; 165(8):2436-49. PMC: 3423248. DOI: 10.1111/j.1476-5381.2011.01380.x. View

Gaus K, Gratton E, Kable E, Jones A, Gelissen I, Kritharides L . Visualizing lipid structure and raft domains in living cells with two-photon microscopy. Proc Natl Acad Sci U S A. 2003; 100(26):15554-9. PMC: 307606. DOI: 10.1073/pnas.2534386100. View

Vuckovic S, Srebro D, Savic Vujovic K, Vucetic C, Prostran M . Cannabinoids and Pain: New Insights From Old Molecules. Front Pharmacol. 2018; 9:1259. PMC: 6277878. DOI: 10.3389/fphar.2018.01259. View

10.

MacDonald J, Pike L . A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res. 2005; 46(5):1061-7. DOI: 10.1194/jlr.D400041-JLR200. View

11.

Gupta B, Hornick M, Briyal S, Donovan R, Prazad P, Gulati A . Anti-apoptotic and Immunomodulatory Effect of CB2 Agonist, JWH133, in a Neonatal Rat Model of Hypoxic-Ischemic Encephalopathy. Front Pediatr. 2020; 8:65. PMC: 7056833. DOI: 10.3389/fped.2020.00065. View

12.

Li X, Serwanski D, Miralles C, Bahr B, De Blas A . Two pools of Triton X-100-insoluble GABA(A) receptors are present in the brain, one associated to lipid rafts and another one to the post-synaptic GABAergic complex. J Neurochem. 2007; 102(4):1329-45. PMC: 2766244. DOI: 10.1111/j.1471-4159.2007.04635.x. View

13.

Mackie K . Cannabinoid receptors: where they are and what they do. J Neuroendocrinol. 2008; 20 Suppl 1:10-4. DOI: 10.1111/j.1365-2826.2008.01671.x. View

14.

Bari M, Battista N, Fezza F, Finazzi-Agro A, Maccarrone M . Lipid rafts control signaling of type-1 cannabinoid receptors in neuronal cells. Implications for anandamide-induced apoptosis. J Biol Chem. 2005; 280(13):12212-20. DOI: 10.1074/jbc.M411642200. View

15.

Sarnataro D, Grimaldi C, Pisanti S, Gazzerro P, Laezza C, Zurzolo C . Plasma membrane and lysosomal localization of CB1 cannabinoid receptor are dependent on lipid rafts and regulated by anandamide in human breast cancer cells. FEBS Lett. 2005; 579(28):6343-9. DOI: 10.1016/j.febslet.2005.10.016. View

16.

Kendall D, Yudowski G . Cannabinoid Receptors in the Central Nervous System: Their Signaling and Roles in Disease. Front Cell Neurosci. 2017; 10:294. PMC: 5209363. DOI: 10.3389/fncel.2016.00294. View

17.

Mackie K . Mechanisms of CB1 receptor signaling: endocannabinoid modulation of synaptic strength. Int J Obes (Lond). 2006; 30 Suppl 1:S19-23. DOI: 10.1038/sj.ijo.0803273. View

18.

Griner L, McGraw K, Johnson J, List A, Reuther G . JAK2-V617F-mediated signalling is dependent on lipid rafts and statins inhibit JAK2-V617F-dependent cell growth. Br J Haematol. 2012; 160(2):177-87. PMC: 4505927. DOI: 10.1111/bjh.12103. View

19.

Mikasova L, Groc L, Choquet D, Manzoni O . Altered surface trafficking of presynaptic cannabinoid type 1 receptor in and out synaptic terminals parallels receptor desensitization. Proc Natl Acad Sci U S A. 2008; 105(47):18596-601. PMC: 2584146. DOI: 10.1073/pnas.0805959105. View

20.

Xie X, Chen J . NMR structural comparison of the cytoplasmic juxtamembrane domains of G-protein-coupled CB1 and CB2 receptors in membrane mimetic dodecylphosphocholine micelles. J Biol Chem. 2004; 280(5):3605-12. DOI: 10.1074/jbc.M410294200. View