Raman Microspectroscopy and Raman Imaging Reveal Biomarkers Specific for Thoracic Aortic Aneurysms

Overview

Journal Cell Rep Med

Publisher Cell Press

Specialties Biology
General Medicine

Date 2021 Jun 7

PMID 34095874

Citations 9

Authors

Kaori Sugiyama

Julia Marzi

Julia Alber

Eva M Brauchle

Masahiro Ando

Yoshito Yamashiro

Bhama Ramkhelawon

Katja Schenke-Layland

Hiromi Yanagisawa

Affiliations

Soon will be listed here.

Abstract

Aortic rupture and dissection are life-threatening complications of ascending thoracic aortic aneurysms (aTAAs), and risk assessment has been largely based on the monitoring of lumen size enlargement. Temporal changes in the extracellular matrix (ECM), which has a critical impact on aortic remodeling, are not routinely evaluated, and cardiovascular biomarkers do not exist to predict aTAA formation. Here, Raman microspectroscopy and Raman imaging are used to identify spectral biomarkers specific for aTAAs in mice and humans by multivariate data analysis (MVA). Multivariate curve resolution-alternating least-squares (MCR-ALS) combined with Lasso regression reveals elastic fiber-derived (Ce1) and collagen fiber-derived (Cc6) components that are significantly increased in aTAA lesions of murine and human aortic tissues. In particular, Cc6 detects changes in amino acid residues, including phenylalanine, tyrosine, tryptophan, cysteine, aspartate, and glutamate. Ce1 and Cc6 may serve as diagnostic Raman biomarkers that detect alterations of amino acids derived from aneurysm lesions.

Citing Articles

Discussion on the comparison of Raman spectroscopy and cardiovascular disease-related imaging techniques and the future applications of Raman technology: a systematic review.

Xie S, Zhu X, Han F, Wang S, Cui K, Xue J Lasers Med Sci. 2025; 40(1):116.

PMID: 39988624 PMC: 11847755. DOI: 10.1007/s10103-025-04315-z.

Tunable Nanomaterials of Intracellular Crystallization for In Situ Biolabeling and Biomedical Imaging.

Liu H, Jiang H, Liu X, Wang X Chem Biomed Imaging. 2024; 1(9):767-784.

PMID: 39473839 PMC: 11504500. DOI: 10.1021/cbmi.3c00021.

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature.

Liu H, Jiang H, Liu X, Wang X Exploration (Beijing). 2024; 3(6):20230033.

PMID: 38264681 PMC: 10742219. DOI: 10.1002/EXP.20230033.

Animal Models, Pathogenesis, and Potential Treatment of Thoracic Aortic Aneurysm.

Wang Y, Panicker I, Anesi J, Sargisson O, Atchison B, Habenicht A Int J Mol Sci. 2024; 25(2).

PMID: 38255976 PMC: 10815651. DOI: 10.3390/ijms25020901.

Translating genomic tools to Raman spectroscopy analysis enables high-dimensional tissue characterization on molecular resolution.

Sigle M, Rohlfing A, Kenny M, Scheuermann S, Sun N, Graessner U Nat Commun. 2023; 14(1):5799.

PMID: 37726278 PMC: 10509269. DOI: 10.1038/s41467-023-41417-0.

References

Ando M, Hamaguchi H . Molecular component distribution imaging of living cells by multivariate curve resolution analysis of space-resolved Raman spectra. J Biomed Opt. 2013; 19(1):011016. DOI: 10.1117/1.JBO.19.1.011016. View

Orringer D, Pandian B, Niknafs Y, Hollon T, Boyle J, Lewis S . Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy. Nat Biomed Eng. 2017; 1. PMC: 5612414. DOI: 10.1038/s41551-016-0027. View

Sajan D, Binoy J, Pradeep B, Venkata Krishna K, Kartha V, Hubert Joe I . NIR-FT Raman and infrared spectra and ab initio computations of glycinium oxalate. Spectrochim Acta A Mol Biomol Spectrosc. 2003; 60(1-2):173-80. DOI: 10.1016/s1386-1425(03)00193-8. View

Shetty G, Kendall C, Shepherd N, Stone N, Barr H . Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus. Br J Cancer. 2006; 94(10):1460-4. PMC: 2361283. DOI: 10.1038/sj.bjc.6603102. View

Sigurdsson S, Philipsen P, Hansen L, Larsen J, Gniadecka M, Wulf H . Detection of skin cancer by classification of Raman spectra. IEEE Trans Biomed Eng. 2004; 51(10):1784-93. DOI: 10.1109/TBME.2004.831538. View

Camp Jr C, Lee Y, Heddleston J, Hartshorn C, Walker A, Rich J . High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues. Nat Photonics. 2015; 8:627-634. PMC: 4304702. DOI: 10.1038/nphoton.2014.145. View

Bergholt M, St-Pierre J, Offeddu G, Parmar P, Albro M, Puetzer J . Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage. ACS Cent Sci. 2017; 2(12):885-895. PMC: 5200931. DOI: 10.1021/acscentsci.6b00222. View

Pope F, Martin G, Lichtenstein J, Penttinen R, Gerson B, Rowe D . Patients with Ehlers-Danlos syndrome type IV lack type III collagen. Proc Natl Acad Sci U S A. 1975; 72(4):1314-6. PMC: 432523. DOI: 10.1073/pnas.72.4.1314. View

Horiguchi M, Inoue T, Ohbayashi T, Hirai M, Noda K, Marmorstein L . Fibulin-4 conducts proper elastogenesis via interaction with cross-linking enzyme lysyl oxidase. Proc Natl Acad Sci U S A. 2009; 106(45):19029-34. PMC: 2776456. DOI: 10.1073/pnas.0908268106. View

10.

Stevens O, Iping Petterson I, Day J, Stone N . Developing fibre optic Raman probes for applications in clinical spectroscopy. Chem Soc Rev. 2016; 45(7):1919-34. DOI: 10.1039/c5cs00850f. View

11.

Wei L, Chen Z, Shi L, Long R, Anzalone A, Zhang L . Super-multiplex vibrational imaging. Nature. 2017; 544(7651):465-470. PMC: 5939925. DOI: 10.1038/nature22051. View

12.

Debelle L, Alix A, Wei S, Jacob M, Huvenne J, Berjot M . The secondary structure and architecture of human elastin. Eur J Biochem. 1999; 258(2):533-9. DOI: 10.1046/j.1432-1327.1998.2580533.x. View

13.

Stone N, Kendall C, Smith J, Crow P, Barr H . Raman spectroscopy for identification of epithelial cancers. Faraday Discuss. 2004; 126:141-57. DOI: 10.1039/b304992b. View

14.

Trimarchi S, Jonker F, Hutchison S, Isselbacher E, Pape L, Patel H . Descending aortic diameter of 5.5 cm or greater is not an accurate predictor of acute type B aortic dissection. J Thorac Cardiovasc Surg. 2011; 142(3):e101-7. DOI: 10.1016/j.jtcvs.2010.12.032. View

15.

Suna G, Wojakowski W, Lynch M, Barallobre-Barreiro J, Yin X, Mayr U . Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries. Circulation. 2017; 137(2):166-183. PMC: 5757669. DOI: 10.1161/CIRCULATIONAHA.116.023381. View

16.

Carvalho L, Nogueira M, Bhattacharjee T, Neto L, Daun L, Mendes T . In vivo Raman spectroscopic characteristics of different sites of the oral mucosa in healthy volunteers. Clin Oral Investig. 2018; 23(7):3021-3031. DOI: 10.1007/s00784-018-2714-5. View

17.

Azeloglu E, Albro M, Thimmappa V, Ateshian G, Costa K . Heterogeneous transmural proteoglycan distribution provides a mechanism for regulating residual stresses in the aorta. Am J Physiol Heart Circ Physiol. 2007; 294(3):H1197-205. DOI: 10.1152/ajpheart.01027.2007. View

18.

Chaichi A, Prasad A, Gartia M . Raman Spectroscopy and Microscopy Applications in Cardiovascular Diseases: From Molecules to Organs. Biosensors (Basel). 2018; 8(4). PMC: 6315865. DOI: 10.3390/bios8040107. View

19.

Frushour B, Koenig J . Raman scattering of collagen, gelatin, and elastin. Biopolymers. 1975; 14(2):379-91. DOI: 10.1002/bip.1975.360140211. View

20.

Zbinden A, Marzi J, Schlunder K, Probst C, Urbanczyk M, Black S . Non-invasive marker-independent high content analysis of a microphysiological human pancreas-on-a-chip model. Matrix Biol. 2019; 85-86:205-220. DOI: 10.1016/j.matbio.2019.06.008. View