A Redox Cu(II)-Graphene Oxide Modified Screen Printed Carbon Electrode As a Cost-Effective and Versatile Sensing Platform for Electrochemical Label-Free Immunosensor and Non-enzymatic Glucose Sensor

Overview

Journal Front Chem

Specialty Chemistry

Date 2021 Jun 7

PMID 34095085

Citations 5

Authors

Sopit Phetsang

Duangruedee Khwannimit

Parawee Rattanakit

Narong Chanlek

Pinit Kidkhunthod

Pitchaya Mungkornasawakul

Jaroon Jakmunee

Kontad Ounnunkad

Affiliations

Soon will be listed here.

Abstract

A novel copper (II) ions [Cu(II)]-graphene oxide (GO) nanocomplex-modified screen-printed carbon electrode (SPCE) is successfully developed as a versatile electrochemical platform for construction of sensors without an additionally external redox probe. A simple strategy to prepare the redox GO-modified SPCE is described. Such redox GO based on adsorbed Cu(II) is prepared by incubation of GO-modified SPCE in the Cu(II) solution. This work demonstrates the fabrications of two kinds of electrochemical sensors, i.e., a new label-free electrochemical immunosensor and non-enzymatic sensor for detections of immunoglobulin G (IgG) and glucose, respectively. Our immunosensor based on square-wave voltammetry (SWV) of the redox GO-modified electrode shows the linearity in a dynamic range of 1.0-500 pg.mL with a limit of detection (LOD) of 0.20 pg.mL for the detection of IgG while non-enzymatic sensor reveals two dynamic ranges of 0.10-1.00 mM (sensitivity = 36.31 μA.mM.cm) and 1.00-12.50 mM (sensitivity = 3.85 μA.mM.cm) with a LOD value of 0.12 mM. The novel redox Cu(II)-GO composite electrode is a promising candidate for clinical research and diagnosis.

Citing Articles

Glucose and UA sensing based on Cu nanoparticle decorated Nif/GO flexible electrode.

Shi F, Hu S, Li J, Wang F, Chen N Mikrochim Acta. 2023; 191(1):7.

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Carbon Nanomaterials-Based Screen-Printed Electrodes for Sensing Applications.

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References

Qiu L, Wang C, Hu P, Wu Z, Shen G, Yu R . A label-free electrochemical immunoassay for IgG detection based on the electron transfer. Talanta. 2010; 83(1):42-7. DOI: 10.1016/j.talanta.2010.08.036. View

Zhu C, Yang G, Li H, Du D, Lin Y . Electrochemical sensors and biosensors based on nanomaterials and nanostructures. Anal Chem. 2014; 87(1):230-49. PMC: 4287168. DOI: 10.1021/ac5039863. View

Yakoh A, Pimpitak U, Rengpipat S, Hirankarn N, Chailapakul O, Chaiyo S . Paper-based electrochemical biosensor for diagnosing COVID-19: Detection of SARS-CoV-2 antibodies and antigen. Biosens Bioelectron. 2020; 176:112912. PMC: 7746088. DOI: 10.1016/j.bios.2020.112912. View

Li F, Li Y, Dong Y, Jiang L, Wang P, Liu Q . An ultrasensitive label-free electrochemical immunosensor based on signal amplification strategy of multifunctional magnetic graphene loaded with cadmium ions. Sci Rep. 2016; 6:21281. PMC: 4754691. DOI: 10.1038/srep21281. View

Ni L, Li Y . Role of graphene oxide in mitigated toxicity of heavy metal ions on . RSC Adv. 2022; 8(72):41358-41367. PMC: 9091651. DOI: 10.1039/c8ra09135h. View

Khristunova E, Dorozhko E, Korotkova E, Kratochvil B, Vyskocil V, Barek J . Label-Free Electrochemical Biosensors for the Determination of : Dengue, Zika, and Japanese Encephalitis. Sensors (Basel). 2020; 20(16). PMC: 7472106. DOI: 10.3390/s20164600. View

Zhang L, Liu Y, Chen T . A mediatorless and label-free amperometric immunosensor for detection of h-IgG. Int J Biol Macromol. 2008; 43(2):165-9. DOI: 10.1016/j.ijbiomac.2008.04.010. View

Yazid S, Isa I, Hashim N . Novel alkaline-reduced cuprous oxide/graphene nanocomposites for non-enzymatic amperometric glucose sensor application. Mater Sci Eng C Mater Biol Appl. 2016; 68:465-473. DOI: 10.1016/j.msec.2016.06.006. View

Tang Z, Fu Y, Ma Z . Multiple signal amplification strategies for ultrasensitive label-free electrochemical immunoassay for carbohydrate antigen 24-2 based on redox hydrogel. Biosens Bioelectron. 2016; 91:299-305. DOI: 10.1016/j.bios.2016.12.049. View

10.

Sridara T, Upan J, Saianand G, Tuantranont A, Karuwan C, Jakmunee J . Non-Enzymatic Amperometric Glucose Sensor Based on Carbon Nanodots and Copper Oxide Nanocomposites Electrode. Sensors (Basel). 2020; 20(3). PMC: 7038693. DOI: 10.3390/s20030808. View

11.

Zhao G, Li J, Ren X, Chen C, Wang X . Few-layered graphene oxide nanosheets as superior sorbents for heavy metal ion pollution management. Environ Sci Technol. 2011; 45(24):10454-62. DOI: 10.1021/es203439v. View

12.

Kim D, Moon J, Lee W, Yoon J, Choi C, Shim Y . A potentiometric non-enzymatic glucose sensor using a molecularly imprinted layer bonded on a conducting polymer. Biosens Bioelectron. 2016; 91:276-283. DOI: 10.1016/j.bios.2016.12.046. View

13.

Montes R, Cespedes F, Baeza M . Highly sensitive electrochemical immunosensor for IgG detection based on optimized rigid biocomposites. Biosens Bioelectron. 2015; 78:505-512. DOI: 10.1016/j.bios.2015.11.081. View

14.

Tan P, Sun J, Hu Y, Fang Z, Bi Q, Chen Y . Adsorption of Cu(2+), Cd(2+) and Ni(2+) from aqueous single metal solutions on graphene oxide membranes. J Hazard Mater. 2015; 297:251-60. DOI: 10.1016/j.jhazmat.2015.04.068. View

15.

Thunkhamrak C, Reanpang P, Ounnunkad K, Jakmunee J . Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G. Talanta. 2017; 171:53-60. DOI: 10.1016/j.talanta.2017.04.058. View

16.

Soldatkin O, Peshkova V, Saiapina O, Kucherenko I, Dudchenko O, Melnyk V . Development of conductometric biosensor array for simultaneous determination of maltose, lactose, sucrose and glucose. Talanta. 2013; 115:200-7. DOI: 10.1016/j.talanta.2013.04.065. View

17.

Wang K, Liu Q, Guan Q, Wu J, Li H, Yan J . Enhanced direct electrochemistry of glucose oxidase and biosensing for glucose via synergy effect of graphene and CdS nanocrystals. Biosens Bioelectron. 2010; 26(5):2252-7. DOI: 10.1016/j.bios.2010.09.043. View

18.

Song J, Xu L, Zhou C, Xing R, Dai Q, Liu D . Synthesis of graphene oxide based CuO nanoparticles composite electrode for highly enhanced nonenzymatic glucose detection. ACS Appl Mater Interfaces. 2013; 5(24):12928-34. DOI: 10.1021/am403508f. View

19.

Vargas E, Teymourian H, Tehrani F, Eksin E, Sanchez-Tirado E, Warren P . Enzymatic/Immunoassay Dual-Biomarker Sensing Chip: Towards Decentralized Insulin/Glucose Detection. Angew Chem Int Ed Engl. 2019; 58(19):6376-6379. DOI: 10.1002/anie.201902664. View

20.

Dong Y, Wilkop T, Xu D, Wang Z, Cheng Q . Microchannel chips for the multiplexed analysis of human immunoglobulin G-antibody interactions by surface plasmon resonance imaging. Anal Bioanal Chem. 2008; 390(6):1575-83. DOI: 10.1007/s00216-008-1849-7. View