Improved Electrophoretic and Immunochemical Techniques for the Identification and Characterization of Mutant Proteins, Applied to Ribosomal Protein S8 in Escherichia Coli Mutants

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Dec 30

PMID 340933

Citations 11

Authors

W Zubke

H Stadler

R Ehrlich

G Stoffler

H G WITTMANN

D Apirion

Affiliations

Soon will be listed here.

Abstract

The ribosomal proteins of 11 mutants which are sensitive to starvation at elevated temperature and of 36 transductants derived from them were studied with several electrophoretic, immunochemical and proteinchemical methods. The following results were obtained: (1) Ribosomal protein S8 is altered in three of these mutants. (2) The amino acid exchange in proteins S8 of mutant N4128 is Glu leads to Lys in position 59 of the protein chain. (3) Temperature sensitivity and inability to recover from starvation at elevated temperatures are caused by the same mutational event which is, however, unrelated to the alteration in protein S8. Several electrophoretic and immunological procedures were applied during the characterization of these mutants. A modified immunoelectrophoresis on cellulose acetate gels was developed, and proved to be the most applicable procedure for the detection of mutationally altered ribosomal proteins. This procedure may gain general importance for detecting mutational alterations in other proteins.

Citing Articles

Arrangement of the subunits in the ribosome of Escherichia coli: demonstration by immunoelectron microscopy.

Kastner B, Stoffler-Meilicke M, Stoffler G Proc Natl Acad Sci U S A. 1981; 78(11):6652-6.

PMID: 7031657 PMC: 349107. DOI: 10.1073/pnas.78.11.6652.

Expression of the L11-L1 operon in mutants of Escherichia coli lacking the ribosomal proteins L1 or L11.

Stoffler G, Hasenbank R, Dabbs E Mol Gen Genet. 1981; 181(2):164-8.

PMID: 7024735 DOI: 10.1007/BF00268422.

Immunological studies of Escherichia coli mutants lacking one or two ribosomal proteins.

Dabbs E, Hasenbank R, Kastner B, Rak K, Wartusch B, Stoffler G Mol Gen Genet. 1983; 192(3):301-8.

PMID: 6361486 DOI: 10.1007/BF00392166.

Immunological comparison of ribosomal proteins from archaebacteria.

Schmid G, Bock A J Bacteriol. 1981; 147(2):282-8.

PMID: 6167563 PMC: 216043. DOI: 10.1128/jb.147.2.282-288.1981.

Location of protein S4 on the small ribosomal subunit of E. coli and B. stearothermophilus with protein- and hapten-specific antibodies.

Stoffler-Meilicke M, Epe B, Woolley P, Lotti M, Littlechild J, Stoffler G Mol Gen Genet. 1984; 197(1):8-18.

PMID: 6083434 DOI: 10.1007/BF00327916.

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