Purification and Properties of a Lambda Operator-binding Protein Which is Expected to Be Autorepressor (tof Protein) from E. Coli Carrying Lambdadv Plasmid

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Nov 29

PMID 340920

Citations 8

Authors

T Murotsu

H Tanaka

M Imaji

H Koga

K Matsubara

T Horiuchi

Affiliations

Soon will be listed here.

Abstract

In order to study the mode of action of the tof gene product, which is an "autorepressor" of the bacteriophage lambda and plasmid lambdadv, we have purified a DNA-binding protein which is specifically produced in bacteria carrying lambdadv. This protein possesses characteristics expected for the product of the tof gene, since it is produced under conditions where cI-repressor is not made, and since it binds to oL and oR operators on the lambda phage genome. The molecular weight of the native protein is 16,000-17,000 daltons, and the monomeric molecular weight as measured by gel electrophoresis in the presence of sodium dodecyl sulfate is about 10,000 daltons. Denaturation and renaturation experiments demonstrated that the native protein is a dimer of 10,000-dalton monomers. The lambdaDNA-specific binding protein is not produced in cells carrying i21dv or ø80dv.

Citing Articles

Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

Komai N, Nishizawa T, Hayakawa Y, Murotsu T, Matsubara K Mol Gen Genet. 1982; 186(2):193-203.

PMID: 7050629 DOI: 10.1007/BF00331850.

Coliphage 434 tof Protein: NH2-terminal amino acid sequence and kinetic and equilibrium measurements of DNA binding.

Aono J, Kangawa K, Matsuo H, Horiuchi T Mol Gen Genet. 1982; 186(4):460-6.

PMID: 6752655 DOI: 10.1007/BF00337948.

Purification of bacteriophage lambda O protein that specifically binds to the origin of replication.

Tsurimoto T, Matsubara K Mol Gen Genet. 1981; 181(3):325-31.

PMID: 6454055 DOI: 10.1007/BF00425606.

Role of an autorepression system in the control of lambda dv plasmid copy number and incompatibility.

Murotsu T, Matsubara K Mol Gen Genet. 1980; 179(3):509-19.

PMID: 6449651 DOI: 10.1007/BF00271740.

Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin.

Tsurimoto T, Matsubara K Nucleic Acids Res. 1981; 9(8):1789-99.

PMID: 6264392 PMC: 326803. DOI: 10.1093/nar/9.8.1789.

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