Long Non-coding RNA XIST Regulates Ovarian Cancer Progression Via Modulating MiR-335/BCL2L2 Axis

Overview

Journal World J Surg Oncol

Publisher Biomed Central

Specialties General Surgery
Oncology

Date 2021 Jun 6

PMID 34090463

Citations 10

Authors

Qingjuan Meng

Ningning Wang

Guanglan Duan

Affiliations

Soon will be listed here.

Abstract

Background: X inactivation-specific transcript (XIST) is the long non-coding RNA (lncRNA) related to cancer, which is involved in the development and progression of various types of tumor. However, up to now, the exact role and molecular mechanism of XIST in the progression of ovarian cancer are not clear. We studied the function of XIST in ovarian cancer cells and clinical tumor specimens.

Methods: RT-qPCR was performed to detect the expression levels of miR-335 and BCL2L2 in ovarian cancer cells and tissues. MTT and transwell assays were carried out to detect cell proliferation, migration, and invasion abilities. Western blot was performed to analyze the expression level of BCL2L2. The interaction between miR-335 and XIST/BCL2L2 was confirmed using a luciferase reporter assay.

Results: The inhibition of XIST can inhibit the proliferation invasion and migration of human ovarian cancer cells. In addition, the miR-335/BCL2L2 axis was involved in the functions of XIST in ovarian cancer cells. These results suggested that XIST could regulate tumor proliferation and invasion and migration via modulating miR-335/BCL2L2.

Conclusion: XIST might be a carcinogenic lncRNA in ovarian cancer by regulating miR-335, and it can serve as a therapeutic target in human ovarian cancer.

Citing Articles

Loss of XIST lncRNA unlocks stemness and cellular plasticity in ovarian cancer.

Naciri I, Liang M, Yang Y, Karner H, Lin B, De Lourdes Andrade Ludena M Proc Natl Acad Sci U S A. 2024; 121(47):e2418096121.

PMID: 39546568 PMC: 11588085. DOI: 10.1073/pnas.2418096121.

Prognostic significance of non-coding RNAs related to the tumorigenic epithelial-mesenchymal transition (EMT) process among ovarian cancer patients: A systematic review and meta-analysis.

Khaboushan A, Salimian S, Mehraban S, Bahramy A, Zafari N, Kajbafzadeh A Heliyon. 2024; 10(16):e35202.

PMID: 39253159 PMC: 11382180. DOI: 10.1016/j.heliyon.2024.e35202.

Diagnostic Value of lncRNA XIST in Saliva for Early Peri-Implantitis.

Chai M, Zhang J, Meng Q, Liu A Oral Health Prev Dent. 2024; 22:381-388.

PMID: 39105315 PMC: 11619896. DOI: 10.3290/j.ohpd.b5656312.

miRNAs: Potential as Biomarkers and Therapeutic Targets for Cancer.

Chakrabortty A, Patton D, Smith B, Agarwal P Genes (Basel). 2023; 14(7).

PMID: 37510280 PMC: 10378777. DOI: 10.3390/genes14071375.

Potential roles of lncRNA-XIST/miRNAs/mRNAs in human cancer cells.

Farzaneh M, Nasrolahi A, Ghaedrahmati F, Masoodi T, Najafi S, Sheykhi-Sabzehpoush M Clin Transl Oncol. 2023; 25(7):2015-2042.

PMID: 36853400 DOI: 10.1007/s12094-023-03110-y.

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