Replication of the Ampicillin Resistance Plasmid RSF1030 in Extracts of Escherichia Coli: Separation of the Replication Cycle into Early and Late Stages

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Nov 4

PMID 340889

Citations 11

Authors

W L Staudenbauer

Affiliations

Soon will be listed here.

Abstract

The replication cycle of the small resistance plasmid RSF1030 can be divided into two stages with different enzyme requirements: (1) Synthesis of early replicative intermediates containing 7 S DNA catalyzed by DNA polymerase I in the absence of functional dnaZ protein, and (2) replication of early intermediates requiring DNA polymerase III holenzyme (including the dnaZ protein). Early intermediate DNA synthesized in a dnaZ extract can be converted to fully replicated plasmid molecules upon addition to a replication enzyme fraction prepared by ammonium sulfate fractionation of polA I extracts. The first-stage reaction is sensitive to rifampicin, novobiocin, and oxolinic acid, but insensitive to arabinosylcytosine triphosphate (aCTP). Addition of aCTP interferes with the second-stage reaction resulting in the accumulation of late replicative intermediates.

Citing Articles

Mechanisms of transcription-replication collisions in bacteria.

Mirkin E, Mirkin S Mol Cell Biol. 2005; 25(3):888-95.

PMID: 15657418 PMC: 544003. DOI: 10.1128/MCB.25.3.888-895.2005.

DNA polymerase beta can substitute for DNA polymerase I in the initiation of plasmid DNA replication.

Sweasy J, Chen M, Loeb L J Bacteriol. 1995; 177(10):2923-5.

PMID: 7751308 PMC: 176970. DOI: 10.1128/jb.177.10.2923-2925.1995.

Evidence for two genetically distinct DNA primase activities specified by plasmids of the B and I incompatibility groups.

Dalrymple B, Boulnois G, Wilkins B, Orr E, Williams P J Bacteriol. 1982; 151(1):1-7.

PMID: 7045070 PMC: 220173. DOI: 10.1128/jb.151.1.1-7.1982.

Discriminatory function of ribonuclease H in the selective initiation of plasmid DNA replication.

Hillenbrand G, Staudenbauer W Nucleic Acids Res. 1982; 10(3):833-53.

PMID: 6278438 PMC: 326206. DOI: 10.1093/nar/10.3.833.

An Escherichia coli mutant thermosensitive in the B subunit of DNA gyrase: effect on the structure and replication of the colicin E1 plasmid in vitro.

Orr E, Staudenbauer W Mol Gen Genet. 1981; 181(1):52-6.

PMID: 6261085 DOI: 10.1007/BF00339004.

References

TOMIZAWA J, Ohmori H, Bird R . Origin of replication of colicin E1 plasmid DNA. Proc Natl Acad Sci U S A. 1977; 74(5):1865-9. PMC: 431032. DOI: 10.1073/pnas.74.5.1865. View

Staudenbauer W . Replication of colicinogenic factor E1 DNA in plasmolysed Escherichia coli cells. Coupling of DNA replication and RNA synthesis. Eur J Biochem. 1975; 58(2):303-13. DOI: 10.1111/j.1432-1033.1975.tb02377.x. View

Weiner J, BERTSCH L, Kornberg A . The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication. J Biol Chem. 1975; 250(6):1972-80. View

Wirtz A, Hofschneider P . Replication of the single stranded DNA bacteriophage M 13. Intracellular flow of parental DNA and transfer to progeny particles. Eur J Biochem. 1970; 17(1):141-50. DOI: 10.1111/j.1432-1033.1970.tb01146.x. View

Truitt C, Walker J . Growth of phages lambda, phiX174, and Ml3 requires the dnaZ (previously dnaH) gene product of Escherichia coli. Biochem Biophys Res Commun. 1974; 61(3):1036-42. DOI: 10.1016/0006-291x(74)90259-9. View

Humphreys G, Willshaw G, Anderson E . A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975; 383(4):457-63. DOI: 10.1016/0005-2787(75)90318-4. View

Sakakibara Y, Tomizawa J . Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates. Proc Natl Acad Sci U S A. 1974; 71(4):1403-7. PMC: 388237. DOI: 10.1073/pnas.71.4.1403. View

Wickner W, Kornberg A . A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties. J Biol Chem. 1974; 249(19):6244-9. View

Filip C, Allen J, Gustafson R, Allen R, Walker J . Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli. J Bacteriol. 1974; 119(2):443-9. PMC: 245626. DOI: 10.1128/jb.119.2.443-449.1974. View

10.

Wickner S, Hurwitz J . Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro. Proc Natl Acad Sci U S A. 1976; 73(4):1053-7. PMC: 430198. DOI: 10.1073/pnas.73.4.1053. View

11.

Kessler C, HARTMANN G . The two effects of rifampicin on the RNA polymerase reaction. Biochem Biophys Res Commun. 1977; 74(1):50-6. DOI: 10.1016/0006-291x(77)91373-0. View

12.

Staudenbauer W . Replication of small plasmids in extracts of Escherichia coli. Mol Gen Genet. 1976; 145(3):273-80. DOI: 10.1007/BF00325823. View

13.

Gellert M, ODea M, Itoh T, Tomizawa J . Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc Natl Acad Sci U S A. 1976; 73(12):4474-8. PMC: 431506. DOI: 10.1073/pnas.73.12.4474. View

14.

Staudenbauer W . Replication of Escherichia coli DNA in vitro: inhibition by oxolinic acid. Eur J Biochem. 1976; 62(3):491-7. DOI: 10.1111/j.1432-1033.1976.tb10183.x. View

15.

Staudenbauer W, Hofschneider P . Membrane attachment of replicating parental DNA molecules of bacteriophage M13. Biochem Biophys Res Commun. 1971; 42(6):1035-41. DOI: 10.1016/0006-291x(71)90008-8. View

16.

Wickner S . Mechanism of DNA elongation catalyzed by Escherichia coli DNA polymerase III, dnaZ protein, and DNA elongation factors I and III. Proc Natl Acad Sci U S A. 1976; 73(10):3511-5. PMC: 431146. DOI: 10.1073/pnas.73.10.3511. View

17.

Low R, Rashbaum S, Cozzarelli N . Purification and characterization of DNA polymerase III from Bacillus subtilis. J Biol Chem. 1976; 251(5):1311-25. View

18.

Staudenbauer W . REPLICAtion of small plasmids in extracts of Escherichia coli: requirement for both DNA polymerases I and II. Mol Gen Genet. 1976; 149(2):151-8. DOI: 10.1007/BF00332883. View

19.

Haldenwang W, Walker J . Parental RF formation on phages phiX174 and M13 requires the dnaZ gene product of Escherichia coli. Biochem Biophys Res Commun. 1976; 70(3):932-8. DOI: 10.1016/0006-291x(76)90681-1. View

20.

Crosa J, Luttropp L, Falkow S . Nature of R-factor replication in the presence of chloramphenicol. Proc Natl Acad Sci U S A. 1975; 72(2):654-8. PMC: 432373. DOI: 10.1073/pnas.72.2.654. View