Molecular Mechanisms of Fat Deposition: is a Hub Gene in Fat Lipolysis, Comparing Thin-tailed with Fat-tailed Sheep Breeds

Overview

Journal Arch Anim Breed

Date 2021 Jun 4

PMID 34084904

Citations 9

Authors

Sana Farhadi

Jalil Shodja Ghias

Karim Hasanpur

Seyed Abolghasem Mohammadi

Esmaeil Ebrahimie

Affiliations

Soon will be listed here.

Abstract

Tail fat content affects meat quality and varies significantly among different breeds of sheep. Ghezel (fat-tailed) and Zel (thin-tailed) are two important Iranian local sheep breeds with different patterns of fat storage. The current study presents the transcriptome characterization of tail fat using RNA sequencing in order to get a better comprehension of the molecular mechanism of lipid storage in the two mentioned sheep breeds. Seven (Zel   4 and Ghezel   3) 7-month-old male lambs were used for this experiment. The results of sequencing were analyzed with bioinformatics methods, including differentially expressed genes (DEGs) identification, functional enrichment analysis, structural classification of proteins, protein-protein interaction (PPI) and network and module analyses. Some of the DEGs, such as , , , , and especially , had a close association with lipid metabolism. Furthermore, functional enrichment analysis revealed pathways associated with fat deposition, including "fatty acid metabolism", "fatty acid biosynthesis" and " signaling pathway". The structural classification of proteins showed that major down-regulated DEGs in the Zel (thin-tailed) breed were classified under transporter class and that most of them belonged to the solute carrier transporter (SLC) families. In addition, DEGs under the transcription factor class with an important role in lipolysis were up-regulated in the Zel (thin-tailed) breed. Also, network analysis revealed that and were hub genes for up-regulated PPI networks, and , and were hub genes for down-regulated PPI networks. Among the up-regulated DEGs, the gene seems to play an important role in lipolysis of tail fat in thin-tailed sheep breeds via various pathways such as tumor necrosis factor (TNF) signaling and mitogen-activated protein kinase (MAPK) signaling pathways. Due to the probable role of the gene in fat lipolysis and also due to the strong interaction of with the other up-regulated DEGs, it seems that accelerates the degradation of lipids in tail fat cells.

Citing Articles

LIPG-mediated regulation of lipid deposition and proliferation in goat intramuscular preadipocytes involves the PPARα signaling pathway.

Wang Y, Huang L, Zhu J, Zhang W, Tang Y, Yang C PLoS One. 2025; 20(2):e0317953.

PMID: 39946436 PMC: 11825097. DOI: 10.1371/journal.pone.0317953.

Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models.

Sun H, Li X, Chen Z, Meng H Front Pharmacol. 2025; 15():1531524.

PMID: 39850562 PMC: 11754250. DOI: 10.3389/fphar.2024.1531524.

Fat-tail allele-specific expression genes may affect fat deposition in tail of sheep.

Mansourizadeh H, Bakhtiarizadeh M, Regitano L, Bruscadin J PLoS One. 2024; 19(12):e0316046.

PMID: 39729475 PMC: 11676558. DOI: 10.1371/journal.pone.0316046.

Effect of , and Their Combination on Growth Performance, Digestive Enzymes, Immunity, and Microbiota in Nile Tilapia ().

Guan M, Guan J, Zhang H, Peng D, Wen X, Zhang X Aquac Nutr. 2024; 2024:1755727.

PMID: 39624516 PMC: 11611409. DOI: 10.1155/2024/1755727.

RNA-Seq based selection signature analysis for identifying genomic footprints associated with the fat-tail phenotype in sheep.

Abbasabadi H, Bakhtiarizadeh M, Moradi M, McEwan J Front Vet Sci. 2024; 11:1415027.

PMID: 39403211 PMC: 11471730. DOI: 10.3389/fvets.2024.1415027.

References

Chen N, Miao L, Lin W, Zou D, Huang L, Huang J . Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity. Front Cardiovasc Med. 2021; 8:631650. PMC: 8163519. DOI: 10.3389/fcvm.2021.631650. View

Bakhtiarizadeh M, Salehi A, Alamouti A, Abdollahi-Arpanahi R, Salami S . Deep transcriptome analysis using RNA-Seq suggests novel insights into molecular aspects of fat-tail metabolism in sheep. Sci Rep. 2019; 9(1):9203. PMC: 6591244. DOI: 10.1038/s41598-019-45665-3. View

Ruderman N, Keller C, Richard A, Saha A, Luo Z, Xiang X . Interleukin-6 regulation of AMP-activated protein kinase. Potential role in the systemic response to exercise and prevention of the metabolic syndrome. Diabetes. 2006; 55 Suppl 2:S48-54. DOI: 10.2337/db06-s007. View

Miao X, Luo Q . Genome-wide transcriptome analysis between small-tail Han sheep and the Surabaya fur sheep using high-throughput RNA sequencing. Reproduction. 2013; 145(6):587-96. DOI: 10.1530/REP-12-0507. View

Yang X, Zhang X, Heckmann B, Lu X, Liu J . Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-α (TNF-α)-induced lipolysis in adipocytes. J Biol Chem. 2011; 286(47):40477-85. PMC: 3220500. DOI: 10.1074/jbc.M111.257923. View

Fuhrmeister J, Zota A, Sijmonsma T, Seibert O, Cingir S, Schmidt K . Fasting-induced liver GADD45β restrains hepatic fatty acid uptake and improves metabolic health. EMBO Mol Med. 2016; 8(6):654-69. PMC: 4888855. DOI: 10.15252/emmm.201505801. View

Kim J, Qu A, Reddy J, Gao B, Gonzalez F . Hepatic oxidative stress activates the Gadd45b gene by way of degradation of the transcriptional repressor STAT3. Hepatology. 2013; 59(2):695-704. PMC: 3880633. DOI: 10.1002/hep.26683. View

Zhao N, Li X, Feng Y, Han J, Feng Z, Li X . The Nuclear Orphan Receptor Nur77 Alleviates Palmitate-induced Fat Accumulation by Down-regulating G0S2 in HepG2 Cells. Sci Rep. 2018; 8(1):4809. PMC: 5859288. DOI: 10.1038/s41598-018-23141-8. View

Xu M, Joo H, Paik Y . Novel functions of lipid-binding protein 5 in Caenorhabditis elegans fat metabolism. J Biol Chem. 2011; 286(32):28111-8. PMC: 3151056. DOI: 10.1074/jbc.M111.227165. View

10.

Chin C, Chen S, Wu H, Ho C, Ko M, Lin C . cytoHubba: identifying hub objects and sub-networks from complex interactome. BMC Syst Biol. 2014; 8 Suppl 4:S11. PMC: 4290687. DOI: 10.1186/1752-0509-8-S4-S11. View

11.

Contreras G, Strieder-Barboza C, de Souza J, Gandy J, Mavangira V, Lock A . Periparturient lipolysis and oxylipid biosynthesis in bovine adipose tissues. PLoS One. 2017; 12(12):e0188621. PMC: 5716552. DOI: 10.1371/journal.pone.0188621. View

12.

Nonogaki K, Fuller G, Fuentes N, MOSER A, Staprans I, Grunfeld C . Interleukin-6 stimulates hepatic triglyceride secretion in rats. Endocrinology. 1995; 136(5):2143-9. DOI: 10.1210/endo.136.5.7720663. View

13.

Moreno-Navarrete J, Jove M, Padro T, Boada J, Ortega F, Ricart W . Adipocyte lipopolysaccharide binding protein (LBP) is linked to a specific lipidomic signature. Obesity (Silver Spring). 2016; 25(2):391-400. DOI: 10.1002/oby.21711. View

14.

Pourlis A . A review of morphological characteristics relating to the production and reproduction of fat-tailed sheep breeds. Trop Anim Health Prod. 2011; 43(7):1267-87. DOI: 10.1007/s11250-011-9853-x. View

15.

Symonds M . Brown adipose tissue growth and development. Scientifica (Cairo). 2013; 2013:305763. PMC: 3820149. DOI: 10.1155/2013/305763. View

16.

Bolger A, Lohse M, Usadel B . Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30(15):2114-20. PMC: 4103590. DOI: 10.1093/bioinformatics/btu170. View

17.

Wang M, Zhao S, Tan M . bZIP transmembrane transcription factor CREBH: Potential role in non-alcoholic fatty liver disease (Review). Mol Med Rep. 2016; 13(2):1455-62. DOI: 10.3892/mmr.2015.4749. View

18.

Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley D . Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 2012; 7(3):562-78. PMC: 3334321. DOI: 10.1038/nprot.2012.016. View

19.

Zhou G, Wang X, Yuan C, Kang D, Xu X, Zhou J . Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep. Biomed Res Int. 2017; 2017:1857580. PMC: 5331317. DOI: 10.1155/2017/1857580. View

20.

Wang Y, Kuo W, Chen C, Lin H, Wu H, Liu B . Docosahexaenoic acid regulates serum amyloid A protein to promote lipolysis through down regulation of perilipin. J Nutr Biochem. 2009; 21(4):317-24. DOI: 10.1016/j.jnutbio.2009.01.004. View