Bio-functional Strontium-containing Photocrosslinked Alginate Hydrogels for Promoting the Osteogenic Behaviors

In recent years, photocrosslinked alginate hydrogel has been widely studied in bone tissue engineering, owing to its numerous advantages. However, there are still some shortcomings like insufficient mechanical strength and lack of bone induction. To compensate for these deficiencies, in this work, a novel doped strontium (Sr) photocrosslinked methacrylated alginate (Sr-PMA) hydrogel was developed. Photocrosslinked alginate hydrogel fabricated via crosslinking methacrylate-modified alginate under ultraviolet (UV) light was placed into strontium solutions to prepare Sr-PMA gel by chelating reaction. The chemical structures, swelling behaviors, degradation profiles, elastic moduli, Sr ion release and surface morphology of the Sr-PMA hydrogel were characterized, and we found that physical properties of the gels can be tailored by varying concentration of Sr ions. And MC3T3-E1 cell viability, proliferation and mineralization outside the hydrogel were also investigated. Further research on cell survival, multiplication, osteogenic differentiation of the cells encapsulated in Sr-PMA hydrogels were explored. In vitro studies of biological properties revealed that incorporation of Sr into photocrosslinked alginate gels significantly improved osteogenic differentiation capabilities and mineralization via stimulating expression of osteogenesis related genes and proteins of the cells compared to strontium-free photocrosslinked alginate gels. The research demonstrates that the innovative Sr-PMA hydrogels possessing adjustable physical performances, excellent biocompatibility and osteogenic differentiation capabilities could be potentially applied to bone tissue engineering and regenerative medicine. Meanwhile, it also provides a reference for the modification of biological properties of biomaterials.