Ang II Promotes Cardiac Autophagy and Hypertrophy Via Orai1/STIM1

Overview

Journal Front Pharmacol

Date 2021 Jun 3

PMID 34079454

Citations 13

Authors

Chang-Bo Zheng

Wen-Cong Gao

Mingxu Xie

Zhichao Li

Xin Ma

Wencong Song

Dan Luo

Yongxiang Huang

Jichen Yang

Peng Zhang

Yu Huang

Weimin Yang

Xiaoqiang Yao

Affiliations

Soon will be listed here.

Abstract

The pathophysiology of cardiac hypertrophy is complex and multifactorial. Both the store-operated Ca entry (SOCE) and excessive autophagy are the major causative factors for pathological cardiac hypertrophy. However, it is unclear whether these two causative factors are interdependent. In this study, we examined the functional role of SOCE and Orai1 in angiotensin II (Ang II)-induced autophagy and hypertrophy using neonatal rat cardiomyocytes (NRCMs) and mouse model, respectively. We show that YM-58483 or SKF-96365 mediated pharmacological inhibition of SOCE, or silencing of Orai1 with Orail-siRNA inhibited Ang II-induced cardiomyocyte autophagy both and . Also, the knockdown of Orai1 attenuated Ang II-induced pathological cardiac hypertrophy. Together, these data suggest that Ang II promotes excessive cardiomyocyte autophagy through SOCE/Orai1 which can be the prime contributing factors in cardiac hypertrophy.

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References

Wollert K, Taga T, Saito M, Narazaki M, Kishimoto T, Glembotski C . Cardiotrophin-1 activates a distinct form of cardiac muscle cell hypertrophy. Assembly of sarcomeric units in series VIA gp130/leukemia inhibitory factor receptor-dependent pathways. J Biol Chem. 1996; 271(16):9535-45. DOI: 10.1074/jbc.271.16.9535. View

Hulot J, Fauconnier J, Ramanujam D, Chaanine A, Aubart F, Sassi Y . Critical role for stromal interaction molecule 1 in cardiac hypertrophy. Circulation. 2011; 124(7):796-805. PMC: 3428713. DOI: 10.1161/CIRCULATIONAHA.111.031229. View

Selvaraj S, Sun Y, Sukumaran P, Singh B . Resveratrol activates autophagic cell death in prostate cancer cells via downregulation of STIM1 and the mTOR pathway. Mol Carcinog. 2015; 55(5):818-31. PMC: 4624064. DOI: 10.1002/mc.22324. View

Gupta S, Das B, Sen S . Cardiac hypertrophy: mechanisms and therapeutic opportunities. Antioxid Redox Signal. 2007; 9(6):623-52. DOI: 10.1089/ars.2007.1474. View

Kimura S, Noda T, Yoshimori T . Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy. 2007; 3(5):452-60. DOI: 10.4161/auto.4451. View

Zheng C, Lo C, Meng Z, Li Z, Zhong M, Zhang P . Gastrodin Inhibits Store-Operated Ca Entry and Alleviates Cardiac Hypertrophy. Front Pharmacol. 2017; 8:222. PMC: 5404510. DOI: 10.3389/fphar.2017.00222. View

Lewis R . Store-operated calcium channels: new perspectives on mechanism and function. Cold Spring Harb Perspect Biol. 2011; 3(12). PMC: 3225942. DOI: 10.1101/cshperspect.a003970. View

Zou Y, Liang Y, Gong H, Zhou N, Ma H, Guan A . Ryanodine receptor type 2 is required for the development of pressure overload-induced cardiac hypertrophy. Hypertension. 2011; 58(6):1099-110. DOI: 10.1161/HYPERTENSIONAHA.111.173500. View

Shaikh S, Troncoso R, Mondaca-Ruff D, Parra V, Garcia L, Chiong M . The STIM1 inhibitor ML9 disrupts basal autophagy in cardiomyocytes by decreasing lysosome content. Toxicol In Vitro. 2018; 48:121-127. DOI: 10.1016/j.tiv.2018.01.005. View

10.

Pan W, Zhong Y, Cheng C, Liu B, Wang L, Li A . MiR-30-regulated autophagy mediates angiotensin II-induced myocardial hypertrophy. PLoS One. 2013; 8(1):e53950. PMC: 3541228. DOI: 10.1371/journal.pone.0053950. View

11.

Tang B, Xia X, Lv X, Yu B, Yuan J, Mai X . Inhibition of Orai1-mediated Ca entry enhances chemosensitivity of HepG2 hepatocarcinoma cells to 5-fluorouracil. J Cell Mol Med. 2016; 21(5):904-915. PMC: 5387165. DOI: 10.1111/jcmm.13029. View

12.

Collins H, Zhu-Mauldin X, Marchase R, Chatham J . STIM1/Orai1-mediated SOCE: current perspectives and potential roles in cardiac function and pathology. Am J Physiol Heart Circ Physiol. 2013; 305(4):H446-58. PMC: 3891250. DOI: 10.1152/ajpheart.00104.2013. View

13.

Bartoli F, Bailey M, Rode B, Mateo P, Antigny F, Bedouet K . Orai1 Channel Inhibition Preserves Left Ventricular Systolic Function and Normal Ca Handling After Pressure Overload. Circulation. 2020; 141(3):199-216. PMC: 6970549. DOI: 10.1161/CIRCULATIONAHA.118.038891. View

14.

Luo X, Hojayev B, Jiang N, Wang Z, Tandan S, Rakalin A . STIM1-dependent store-operated Ca²⁺ entry is required for pathological cardiac hypertrophy. J Mol Cell Cardiol. 2011; 52(1):136-47. PMC: 3247164. DOI: 10.1016/j.yjmcc.2011.11.003. View

15.

Qi H, Ren J, Ba L, Song C, Zhang Q, Cao Y . MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPARγ/NF-κB. Mol Ther Nucleic Acids. 2020; 19:507-522. PMC: 6951838. DOI: 10.1016/j.omtn.2019.12.003. View

16.

Tanwar J, Motiani R . Role of SOCE architects STIM and Orai proteins in Cell Death. Cell Calcium. 2017; 69:19-27. DOI: 10.1016/j.ceca.2017.06.002. View

17.

Zhu Z, Yu T, Liu H, Jin J, He J . SOCE induced calcium overload regulates autophagy in acute pancreatitis via calcineurin activation. Cell Death Dis. 2018; 9(2):50. PMC: 5833430. DOI: 10.1038/s41419-017-0073-9. View

18.

Hunton D, Lucchesi P, Pang Y, Cheng X, DellItalia L, Marchase R . Capacitative calcium entry contributes to nuclear factor of activated T-cells nuclear translocation and hypertrophy in cardiomyocytes. J Biol Chem. 2002; 277(16):14266-73. DOI: 10.1074/jbc.M107167200. View

19.

Parys J, Decuypere J, Bultynck G . Role of the inositol 1,4,5-trisphosphate receptor/Ca2+-release channel in autophagy. Cell Commun Signal. 2012; 10(1):17. PMC: 3413604. DOI: 10.1186/1478-811X-10-17. View

20.

Chen T, Zhu J, Zhang C, Huo K, Fei Z, Jiang X . Protective effects of SKF-96365, a non-specific inhibitor of SOCE, against MPP+-induced cytotoxicity in PC12 cells: potential role of Homer1. PLoS One. 2013; 8(1):e55601. PMC: 3561331. DOI: 10.1371/journal.pone.0055601. View